Identification and use of potential bacterial organic antifungal volatiles in biocontrol

Fernando, W. G. Dilantha; Ramarathnam, Rajesh; Krishnamoorthy, Akkanas S.; Savchuk, Sarah C.

doi:10.1016/j.soilbio.2004.10.021

Cited by 466 publications

(339 citation statements)

References 34 publications

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“…[11][12][13] Organic compounds such as allyl alcohol inhibit germination of S. sclerotiorum, benzothiazole, cyclohexanol, n-decanal, dimethyl trisulfide, 2-ethyl-1-hexanol and nonanal of pseudomonades inhibit mycel growth of S. sclerotiorum, or not further identified compounds of B. subtilis caused structural deformations of pathogenic fungi. 8,14,15 Our survey supports and extends the previous observations that rhizobacteria produce biologically relevant organic volatile compounds. Beside pseudomonades and B. subtilis particularly potent volatiles are emitted by Serratia odorifera, S. plymuthica, Stenotrophomonas maltophilia, S. rhizophila and Burkholderia cepacia.…”

Section: Discussionsupporting

confidence: 87%

“…tritici, and Sclerotinia sclerotiorum. 1,7,8 A comprehensive overview of the compounds, which are emitted from bacteria was recently published. 9 In a previous survey we could show, that rhizobacterial isolates comprising Serratia plymuthica, S. odorifera, Stenotrophomonas maltophilia, S. rhizophila, Pseudomonas fluorescens and P. trivialis synthesize and emit complex blends of volatiles, which confer growth inhibitions to R. solani.…”

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confidence: 99%

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The growth of fungi andArabidopsis thalianais influenced by bacterial volatiles

Kai

Vespermann

Piechulla

2008

Plant Signaling & Behavior

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Dual culture systems, which only allowed volatiles to cross the boundary of a bipartite Petri dish, were used to investigate the effects of bacterial volatiles on the growth of 14 fungi and A. thaliana. The majority of tested combinations exhibited dramatic growth retardations of fungi and A. thaliana, indicating that volatiles can act as antibiotics. It therefore can be concluded that bacterial volatiles influence the growth conditions of organisms in a community and in a habitat.

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Section: Discussionsupporting

confidence: 87%

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confidence: 99%

The growth of fungi andArabidopsis thalianais influenced by bacterial volatiles

Kai

Vespermann

Piechulla

2008

Plant Signaling & Behavior

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“…All three compounds have repeatedly been reported in the context of plant pathogenesis: nonanal and 2-ethyl hexanol were found in the headspace of potato (Solanum tuberosum) tubers infected with different fungi (de Lacy Costello et al, 2001) and of various biological control bacteria (Dilantha Fernando et al, 2005), nonanal was released from whiteflyinfected beans (Phaseolus vulgaris; Birkett et al, 2003), and both nonanal and 2-ethyl hexanol were reported to have in vitro antifungal (Dilantha Fernando et al, 2005) and bactericidal (Nakamura and Hatanaka, 2002) activities.…”

Section: Discussionmentioning

confidence: 91%

“…However, although 2-ethyl hexanol has repeatedly been reported in the context of pathogenesis (de Lacy Costello et al, 2001;Nakamura and Hatanaka, 2002;Dilantha Fernando et al, 2005), the compound is also a very common contaminant. In fact, no convincing study has ever reported a clear case of plant-derived 2-ethyl hexanol (W. Boland, personal communication).…”

Section: Discussionmentioning

confidence: 99%

Airborne Induction and Priming of Plant Defenses against a Bacterial Pathogen

Heil²,

et al. 2009

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Herbivore-induced plant volatiles affect the systemic response of plants to local damage and hence represent potential plant hormones. These signals can also lead to "plant-plant communication," a defense induction in yet undamaged plants growing close to damaged neighbors. We observed this phenomenon in the context of disease resistance. Lima bean (Phaseolus lunatus) plants in a natural population became more resistant against a bacterial pathogen, Pseudomonas syringae pv syringae, when located close to conspecific neighbors in which systemic acquired resistance to pathogens had been chemically induced with benzothiadiazole (BTH). Airborne disease resistance induction could also be triggered biologically by infection with avirulent P. syringae. Challenge inoculation after exposure to induced and noninduced plants revealed that the air coming from induced plants mainly primed resistance, since expression of PATHOGENESIS-RELATED PROTEIN2 (PR-2) was significantly stronger in exposed than in nonexposed individuals when the plants were subsequently challenged by P. syringae. Among others, the plant-derived volatile nonanal was present in the headspace of BTH-treated plants and significantly enhanced PR-2 expression in the exposed plants, resulting in reduced symptom appearance. Negative effects on growth of BTH-treated plants, which usually occur as a consequence of the high costs of direct resistance induction, were not observed in volatile organic compound-exposed plants. Volatile-mediated priming appears to be a highly attractive means for the tailoring of systemic acquired resistance against plant pathogens.

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“…As such, studies on beneficial inhibitory volatile metabolites are relatively new (Chen et al, 2008;El-Hasan et al, 2009;Fernando et al, 2005). Nevertheless, the role of volatile inhibitory compounds in biological control remains to be investigated and may have tremendous potential as the volatile compounds can be entrapped or immobilized in bioformulations and manipulated for applications in the field or for post-harvest disease management (misting, spraying and droplets).…”

Section: Introductionmentioning

confidence: 99%

Identification of Volatile Metabolites from Fungal Endophytes with Biocontrol Potential towards <i>Fusarium oxysporum</i> F. sp. <i>cubense</i> Race 4

Ting¹,

Mah

Tee

2010

American Journal of Agricultural and Biological Sciences

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Problem statement: Fungal endophytes are widely studied for their potential as biocontrol agents towards fungal pathogens. In vitro assessments usually reveal their antibiosis and mycoparasitism nature, but little is understood regarding their production of volatile metabolites as mechanisms of antagonism. Approach: This study explored the potential of fungal endophytes in controlling the pathogen responsible for Fusarium wilt disease. Nine fungal endophytes were tested for their ability to inhibit the growth of the pathogenic Fusarium oxysporum F. sp. cubense race 4 (FocR4) via production of volatile inhibitory metabolites. The type of volatile metabolites produced were subsequently characterized and identified using the Gas-Chromatography Mass-Spectrophotometry (GCMS). Results: Eight of the isolates (BTF05, BTF07, BTF08, BTF15, BTF21, WAA03, WAA02, MIF01) showed positive results with percentages of inhibition varying from 1.43-31.43% while one isolate (ALF01), showed negative result (0% inhibition). Volatile profiles showed that these fungal endophytes produced between 15-47 volatile metabolites per isolate. However, the more volatile metabolites produced by a single endophyte does not indicate better biocontrol potential. Isolate BTF05 produced 47 different volatile metabolites, but has only 8.57% inhibition, compared to isolate BTF21 with 15 metabolites but a percentage of 11.43% inhibition. The potency of the volatile metabolites produced may also influenced the biocontrol potential of the fungal endophytes as some isolates such as BTF08 and MIF01 have only two to three known inhibitory metabolites but have higher PIDG values at 31.43 and 11.43%, respectively. Contrary, isolates WAA02 and WAA03 which has five to six metabolites but PIDG values of less than 3%. Conclusion: Fungal endophytes have the ability to produce several types of volatile metabolites to inhibit the growth of FocR4. These volatile inhibitory metabolites can be further extracted and the amount produced ascertained for future manipulation in biological control of FocR4.

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Identification and use of potential bacterial organic antifungal volatiles in biocontrol

Cited by 466 publications

References 34 publications

The growth of fungi andArabidopsis thalianais influenced by bacterial volatiles

The growth of fungi andArabidopsis thalianais influenced by bacterial volatiles

Airborne Induction and Priming of Plant Defenses against a Bacterial Pathogen

Identification of Volatile Metabolites from Fungal Endophytes with Biocontrol Potential towards <i>Fusarium oxysporum</i> F. sp. <i>cubense</i> Race 4

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