Identification and validation of a novel necroptosis-related prognostic signature in cervical squamous cell carcinoma and endocervical adenocarcinoma

Abstract

Background: The purpose of this study was to investigate the prognostic signature of necroptosis-related lncRNAs (NRLs) and explore their association with immune-related functions and sensitivity of the therapeutic drug in cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC).Methods: UCSC Xena provided lncRNA sequencing and clinical data about CESC, and a necroptosis gene list was obtained from the KEGG database. NRLs were selected by structuring a co-expression network of lncRNAs and necroptosis-related genes. To further screen lncRNAs, we used the univariate Cox regression method, Lasso regression, and multivariate Cox regression. Afterward, an NRL signature was established. We used the xCell algorithm and single-sample gene set enrichment analysis (ssGSEA) to clarify the pertinence between immune infiltration and NRL expressions in CESC patients and explored the relationship between the target lncRNAs and immune-related genes. By leveraging the GDSC database, the therapysensitive response of the prognostic signature was forecasted and an experimental validation was performed. We performed GSEA with the aim of recognizing the potential pathway related to the individual prognostic signature. Results:The two prognostic NRLs (AC009095.1 and AC005332.4) showed significant diversity and constituted the NRL signature. On the grounds of our signature, risk score was an independent element which was bound up with patient outcome (HR = 4.97 CI: 1.87-13.2, P = 0.001). The CESC patients were classified by the median risk score. Immune infiltration analysis revealed significant increases in CD4 + Tcm, eosinophils, epithelial cells, fibroblasts, Frontiers in Oncology frontiersin.org 01

Identification and Validation of the Anoikis-Related Gene Signature as a Novel Prognostic Model for Cervical Squamous Cell Carcinoma, Endocervical Adenocarcinoma, and Revelation Immune Infiltration

“…The human cervical squamous cell carcinoma cell line (SiHa), the human cervical adenocarcinoma cell line (Hela), the normal human cervix cell line (HUCEC), and the human pancreatic cancer cell line (PANC-1) were all acquired from the Shanghai FuHeng Biotechnology Corporation (Shanghai, China). The incubation conditions of the cells and the qRT-PCR were as described in our previous study [ 25 ]. Sequences of the primers are presented in Supplementary File S3: Table S2 .…”

Identification and validation of a novel anoikis-related signature for predicting prognosis and immune landscape in ovarian serous cystadenocarcinoma

“…Subsequently, the hub ARGs were selected via the multivariate regression method and subsequently utilized to establish the risk model. The risk score computation relies on the subsequent formula [ 14 ] (βi indicates the coefficients of each signature. Expi indicates the expression of each signature)…”

Identification of a Necroptosis-Related Prognostic Signature and Associated Regulatory Axis in Lung Adenocarcinoma

“…Necroptosis regulators may also provide prognostic information for cancer and other diseases [9,10]. Moreover, some necroptosis-related prognostic signature had been identified for types of cancer, including stomach adenocarcinoma, breast cancer and cervical squamous cell carcinoma, and endocervical adenocarcinoma [11][12][13]. As such, necroptosis-related genes may also contribute to LUAD prognosis.…”