Identification and validation of an ECERIFERUM2- LIKE gene controlling cuticular wax biosynthesis in cabbage (Brassica oleracea L. var. capitata L.)

Ji, Jialei; Cao, Wenxue; Tong, Li‐Tao; Fang, Zhiyuan; Zhang, Yangyong; Zhuang, Mu; Yong, Wang; Yang, Limei; Lv, Honghao

doi:10.1007/s00122-021-03947-3

Cited by 12 publications

(13 citation statements)

References 54 publications

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“…In the present study, the MFCW of wax gourd could be controlled by a single dominant gene BhWAX , which is in accordance with most of previous studies on other plant species [ 19 , 23 , 39 ]. Recently, BSA-seq has been widely used in the rapid and efficient identification of QTL or genes related to specific traits [ 40 – 42 ].…”

Section: Discussionsupporting

confidence: 92%

Genome-wide association study and genetic mapping of BhWAX conferring mature fruit cuticular wax in wax gourd

Yan

Chen²,

Sun³

et al. 2022

BMC Plant Biol

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Background Wax gourd [Benincasa hispida (Thunb) Cogn. (2n = 2x = 24)] is an economically important vegetable crop of genus Benincasa in the Cucurbitaceae family. Fruit is the main consumption organ of wax gourd. The mature fruit cuticular wax (MFCW) is an important trait in breeding programs, which is also of evolutionary significance in wax gourd. However, the genetic architecture of this valuable trait remains unrevealed. Results In this study, genetic analysis revealed that the inheritance of MFCW was controlled by a single gene, with MFCW dominant over non-MFCW, and the gene was primarily named as BhWAX. Genome-wide association study (GWAS) highlighted a 1.1 Mb interval on chromosome 9 associated with MFCW in wax gourd germplasm resources. Traditional fine genetic mapping delimited BhWAX to a 0.5 Mb region containing 12 genes. Based on the gene annotation, expression analysis and co-segregation analysis, Bhi09G001428 that encodes a membrane bound O-acyltransferase (MBOAT) was proposed as the candidate gene for BhWAX. Moreover, it was demonstrated that the efficiency of a cleaved amplified polymorphic sequences (CAPS) marker in the determination of MFCW in wax gourd reached 80%. Conclusions In closing, the study identified the candidate gene controlling MFCW and provided an efficient molecular marker for the trait in wax gourd for the first time, which will not only be beneficial for functional validation of the gene and marker-assisted breeding of wax gourd, but also lay a foundation for analysis of its evolutionary meaning among cucurbits.

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Section: Discussionsupporting

confidence: 92%

Genome-wide association study and genetic mapping of BhWAX conferring mature fruit cuticular wax in wax gourd

Yan

Chen²,

Sun³

et al. 2022

BMC Plant Biol

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“…Wax contents in plants are essential for hybridization breeding and commercial application. Recently, the accumulation of wax in Brassica vegetables has become a hot research topic [ 20 , 21 ]. Ornamental kale is a popular vegetable and decorative plant whose cuticular wax contents affect its economic value.…”

Section: Discussionmentioning

confidence: 99%

Inactivation of BoORP3a, an oxysterol-binding protein, causes a low wax phenotype in ornamental kale

Zhang

Zhou

Liu

et al. 2022

Horticulture Research

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Identifying genes associated with wax deposition may contribute to the genetic improvement of ornamental kale. Here, we characterized a candidate gene for wax contents, BoORP3a, encoding an oxysterol-binding protein. We sequenced the BoORP3a gene and coding sequence from the high-wax line S0835 and the low-wax line F0819, which revealed 12 single nucleotide polymorphisms between the two lines, of which six caused five amino acids substitutions. BoORP3a appeared to be relatively well conserved in Brassicaceae, as determined by a phylogenetic analysis, and localized to the endoplasmic reticulum and the nucleus. To confirm the role of BoORP3a in wax deposition, we generated three orp3a mutants in a high-wax kale background via CRISPR/Cas9-mediated genome editing. Importantly, all three mutants exhibited lower wax contents and glossy leaves. Overall, these data suggest that BoORP3a may participate in cuticular wax deposition in ornamental kale.

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“…For economically important leafy vegetables in Brassica species, such as Brassica rapa (A genome) and Brassica oleracea (C genome), the waxless and glossy phenotype is an important commodity trait in breeding [23,24]. Therefore, identifying genes responsible for the glossy phenotype facilitates the breeding of new varieties with a glossy green phenotype.…”

Section: Introductionmentioning

confidence: 99%

BrWAX3, Encoding a β-ketoacyl-CoA Synthase, Plays an Essential Role in Cuticular Wax Biosynthesis in Chinese Cabbage

Shu

Tang

Wei

et al. 2022

IJMS

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In this study, we identified a novel glossy mutant from Chinese cabbage, named SD369, and all wax monomers longer than 26 carbons were significantly decreased. Inheritance analysis revealed that the glossy trait of SD369 was controlled by a single recessive locus, BrWAX3. We fine-mapped the BrWAX3 locus to an interval of 161.82 kb on chromosome A09. According to the annotated genome of Brassica rapa, Bra024749 (BrCER60.A09), encoding a β-ketoacyl-CoA synthase, was identified as the candidate gene. Expression analysis showed that BrCER60.A09 was significantly downregulated in all aerial organs of glossy plants. Subcellular localization indicated that the BrCER60.A09 protein functions in the endoplasmic reticulum. A 5567-bp insertion was identified in exon 1 of BrCER60.A09 in SD369, which lead to a premature stop codon, thus causing a loss of function of the BrCER60.A09 enzyme. Moreover, comparative transcriptome analysis revealed that the ‘cutin, suberine, and wax biosynthesis’ pathway was significantly enriched, and genes involved in this pathway were almost upregulated in glossy plants. Further, two functional markers, BrWAX3-InDel and BrWAX3-KASP1, were developed and validated. Overall, these results provide a new information for the cuticular wax biosynthesis and provide applicable markers for marker-assisted selection (MAS)-based breeding of Brassica rapa.

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Identification and validation of an ECERIFERUM2- LIKE gene controlling cuticular wax biosynthesis in cabbage (Brassica oleracea L. var. capitata L.)

Cited by 12 publications

References 54 publications

Genome-wide association study and genetic mapping of BhWAX conferring mature fruit cuticular wax in wax gourd

Genome-wide association study and genetic mapping of BhWAX conferring mature fruit cuticular wax in wax gourd

Inactivation of BoORP3a, an oxysterol-binding protein, causes a low wax phenotype in ornamental kale

BrWAX3, Encoding a β-ketoacyl-CoA Synthase, Plays an Essential Role in Cuticular Wax Biosynthesis in Chinese Cabbage

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