Identification and validation of potential prognostic and predictive miRNAs of epithelial ovarian cancer

Prahm, Kira Philipsen; Høgdall, Claus; Karlsen, Mona Aarenstrup; Christensen, Ib Jarle; Novotny, Guy Wayne; Høgdall, Estrid

doi:10.1371/journal.pone.0207319

Cited by 41 publications

(44 citation statements)

References 57 publications

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“…Numerous studies have shown that miRNA can be used to predict the prognosis of patients with ovarian cancer [85]. Gao et al [86] collected serum samples from 74 patients with epithelial ovarian cancer, 19 patients with borderline ovarian cancer, and 50 healthy controls, and they determined the levels of miR-200c and miR-142 by quantitative PCR.…”

Section: Prognosis Of Mirna and Ovarian Cancermentioning

confidence: 99%

MicroRNA in Ovarian Cancer: Biology, Pathogenesis, and Therapeutic Opportunities

Chen

Chang

Lin

et al. 2019

IJERPH

108

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Ovarian cancer comprises one of the three major malignant tumor types in the female reproductive system. The mortality rate of this cancer is the highest among all gynecological tumors, with ovarian cancer metastasis constituting an important cause of death. Therefore, markers for disease prediction and prognosis are highly desirable for early diagnosis as well as for helping optimize and personalize treatment. Recently, microRNAs (miRNAs), which consist of short-sequence RNAs that do not encode a protein, have emerged as new biomarkers in the clinical diagnosis and treatment of ovarian cancer. By pairing with bases specific to the target messenger RNA (mRNA), miRNAs cause degradation of the target mRNA or inhibit its translation, thereby regulating various cellular processes including cell proliferation and adhesion. Increasing numbers of studies have shown that miRNA expression abnormality plays an important role in the development of ovarian cancer. In this review, we discuss the mechanisms of miRNA action, current research regarding their role in the suppression or promotion of ovarian cancer, and their use as markers for diagnosis of prognosis or as therapeutic targets for this disease. Finally, we present future perspectives regarding the clinical management of ovarian cancer and the role for miRNAs therein.

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Section: Prognosis Of Mirna and Ovarian Cancermentioning

confidence: 99%

MicroRNA in Ovarian Cancer: Biology, Pathogenesis, and Therapeutic Opportunities

Chen

Chang

Lin

et al. 2019

IJERPH

108

View full text Add to dashboard Cite

show abstract

“…Many studies have been conducted to search for cancer biomarkers using mRNA from cryopreserved ovarian tissue or section staining of the tissue with antibodies [ 16 , 18 , 34 , 35 ]. Different research groups have started conducting studies to search for circulating miRNAs [ 36 , 37 ] or miRNAs in the tissue [ 20 , 38 , 39 , 40 ] as specific cancer biomarkers, but a study of miRNA expression after cryopreservation has just been recently published [ 19 ]. This study showed that upregulated miRNA–24 and downregulated expression of miRNA–193b, miRNA–320A, and steroid production could be non-invasive (expression patterns in culture media) markers for cell damage.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, currently, disparities in steroid production and altered expression of specific microRNAs (miRNAs), e.g., miRNA–193b, miRNA–320A, and miRNA–24, after cryopreservation have been associated with failure of ovarian tissue functioning and folliculogenesis [ 19 ]. MicroRNAs are a class of small non–coding RNA molecules that have been shown to be potential biomarkers for different cancers and prognostics [ 20 ] and are involved in gametogenesis, embryo development, and fertility [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Slow-Freezing Cryopreservation Ensures High Ovarian Tissue Quality Followed by In Vivo and In Vitro Methods and Is Safe for Fertility Preservation

et al. 2020

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Background and objectives: Cancer incidence is growing with younger patients diagnosed with this disease every year. Improved cancer diagnostics and treatment lead to better survival of cancer patients. However, after aggressive chemo- or radiotherapy, cancer survivors suffer from various degrees of subfertility or infertility. Several fertility preservation technologies have been developed for young cancer patients: cryopreservation of germ cells, embryos, or reproductive tissues. The best results have been shown by cryopreservation of sperm and embryos. Yet the success of using cryopreserved oocytes or reproductive tissues (ovarian and testicular) is still insufficient. Therefore, this study was designed to assess the vitality, viability, general quality, and safety of frozen–thawed human ovarian tissue for retransplantation using modern molecular tests. Materials and Methods: The new miRNA array test was used to evaluate miRNA expression in thawed ovarian tissue in combination with standard xenotransplantation and pathological examination of microslides. Results: Our results demonstrated that slow freezing is an efficient way (80%) to cryopreserve ovarian tissue with no structural damage afterwards. We have shown that xenotransplantation into immunodeficient mice, histology, and immunohistochemistry could be potentially replaced by more recent molecular methods. Conclusions: The latter method has shown that altered expression of miRNAs might be used as identifiers of normal/damaged tissue after further analysis. Newer, safer, and more specific approaches need to be developed in order to eliminate the risk of disease reoccurrence.

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“…Numerous studies have emphasized the core position of miRNAs in governing cancer development (13,14). miR-1183 was reported to be dysregulated in numerous human diseases (15)(16)(17) and Zhou et al (18) reported that miR-1183 functioned in the tumorigenesis of NSCLC. Nevertheless, the precise mechanism of miR-1183 in NScLc progression remains to be studied.…”

Section: Introductionmentioning

confidence: 99%

Circular RNA circ‑ACACA regulates proliferation, migration and glycolysis in non‑small‑cell lung�carcinoma via miR‑1183 and PI3K/PKB pathway

et al. 2020

Int J Mol Med

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Non-small cell lung carcinoma (NScLc) accounts for 85% of all lung cancers and the five-year survival rate is ~1% in the late stage. circular RNAs (circRNAs) were reported to be involved in the progression of diverse human cancers. However, the role of circ-ACACA in NSCLC progression remains elusive. Quantitative polymerase chain reaction was conducted to detect the expression levels of circ-ACACA and microRNA (miR)-1183 in NScLc tissues and cells. A Cell Counting Kit-8 assay and transwell assay were employed to check proliferation and migration, respectively. Metabolic alternations in NSCLC cells were monitored by the Seahorse XFe96 analyzer. The protein levels of cellular myelocytomatosis, matrix metallopeptidase 9, glucose transporter 1, phosphatase and tensin homolog, phosphoinositide 3-kinases (PI3K), phosphorylated PI3K (p-PI3K), protein kinase B (PKB) and p-PKB in samples were measured by western blotting. The interaction between circ-AcAcA and miR-1183 was predicted by circular RNA Interactome, which was verified by dual-luciferase reporter assay, RNA immunoprecipitation assay and RNA pull-down assay. Xenograft tumor model was established to investigate the biological roles of circ-ACACA in vivo. The level of circ-ACACA was markedly upregulated in NSCLC tissues and cells, which was contrary to the expression of miR-1183. Knockdown of circ-AcAcA inhibited proliferation and migration of NScLc cells and also reduced the glycolysis rate. In addition, miR-1183 was a target of circ-ACACA and its downregulation reversed circ-ACACA silencing-mediated inhibitory impact on NSCLC progression. Further studies indicated that circ-AcAcA regulated the PI3K/PKB pathway through interacting with miR-1183 and downregulation of circ-AcAcA suppressed tumor growth. Knockdown of circ-ACACA impeded NSCLC progression by sponging miR-1183 and inactivating the PI3K/PKB signaling pathway.

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Identification and validation of potential prognostic and predictive miRNAs of epithelial ovarian cancer

Cited by 41 publications

References 57 publications

MicroRNA in Ovarian Cancer: Biology, Pathogenesis, and Therapeutic Opportunities

MicroRNA in Ovarian Cancer: Biology, Pathogenesis, and Therapeutic Opportunities

Slow-Freezing Cryopreservation Ensures High Ovarian Tissue Quality Followed by In Vivo and In Vitro Methods and Is Safe for Fertility Preservation

Circular RNA circ‑ACACA regulates proliferation, migration and glycolysis in non‑small‑cell lung�carcinoma via miR‑1183 and PI3K/PKB pathway

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