Identification and virulence of <i>Chryseobacterium indologenes</i>
 isolated from diseased yellow perch (<i>Perca flavescens</i>
)

Pridgeon, Julia W.; Klesius, Phillip H.; García, Julio C.

doi:10.1111/jam.12070

Cited by 30 publications

(25 citation statements)

References 19 publications

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“…worldwide suggest that chryseobacteria are emerging as sources of disease outbreaks in farmed fishes. The findings from this study, in conjunction with our recent findings (Loch et al 2013, Loch & Faisal 2014a) and those of Pridgeon et al (2013), definitively demonstrate that a diversity of chryseobacteria are also associated with North Ame - viscerum, and C. cha ponense were identified in Great Lakes fish stocks, each of which have been previously isolated from diseased salmonids in Chile and Finland (Ilardi et al 2009(Ilardi et al , 2010, diseased rainbow trout Oncorhynchus mykiss in Spain (Zamora et al 2012c), and diseased Atlantic salmon in Chile (Kämpfer et al 2011). In all cases, the original isolations of these novel chryseobacteria were from farmed fishes (Ilardi et al 2009, Kämpfer et al 2011, Zamora et al 2012c, possibly indicating that the stressors associated with aquaculture are necessary for chryseobacteriosis to ensue; however, this study shows that C. chaponense-like and C. viscerum-like bacteria are also associated with wild fishes.…”

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 85%

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Polyphasic characterization reveals the presence of novel fish-associated Chryseobacterium spp. in the Great Lakes of North America

Loch¹,

Faisal

2015

Dis. Aquat. Org.

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Recent reports suggest an emergence of novel Chryseobacterium spp. associated with aquaculture-reared fish worldwide. Herein, we report on multiple Chryseobacterium spp. infecting Great Lakes fishes that are highly similar to previously detected isolates from Europe, Africa, and Asia but have never before been reported in North America. Polyphasic characterization, which included extensive physiological, morphological, and biochemical analyses, fatty acid profiling, and phylogenetic analyses based upon partial 16S rRNA gene sequences, highlighted the diversity of Great Lakes' fish-associated chryseobacteria and also suggested that at least 2 taxa represent potentially novel Chryseobacterium spp. Screening for the ability of representative chryseobacteria to elicit lesions in experimentally challenged fish showed that they induced varying degrees of pathology, some of which were severe and resulted in host death. Median lethal dose (LD 50 ) experiments for the isolate that elicited the most extensive pathology (Chryseobacterium sp. T28) demonstrated that the LD 50 exceeded 4.5 × 10 8 cfu, thereby suggesting its role as a facultative fish-pathogenic bacterium. Histopathological changes in T28-infected fish included epithelial hyperplasia of the secondary lamellae and interlamellar space that resulted in secondary lamellar fusion, monocytic infiltrate, and mucus cell hyperplasia, all of which are consistent with branchitis, along with monocytic myositis, hemorrhage within the muscle, liver, adipose tissue, and ovaries, spongiosis of white matter of the brain, multifocal edema within the granular cell layer of the cerebellar cortex, and renal tubular degeneration and necrosis. The findings of this study underscore the widespread presence of chryseobacteria infecting Great Lakes fish. KEY WORDS: Chryseobacterium · Fish disease · Great Lakes · Bacterial infections · Flavobacterium Resale or republication not permitted without written consent of the publisherDis Aquat Org 113: [113][114][115][116][117][118][119][120][121][122][123][124][125] 2015 taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes (Bernardet et al. 2002).Paralleling the expansion of the genus, an increasing number of Chryseobacterium spp. have recently been described that are associated with diseased farmed fish worldwide, including C. piscicola (Ilardi et al. 2009), C. chaponense (Kämpfer et al. 2011), C. tructae (Zamora et al. 2012b), C. oncorhynchi (Zamora et al. 2012a), and C. viscerum (Zamora et al. 2012c). During these outbreaks, external lesions (Kämpfer et al. 2011), deep ulcerations (Ilardi et al. 2010, and signs of septicemia (Zamora et al. 2012a,b) were reported in infected fish. Unfortunately, unraveling the pathogenesis of these novel fish-pathogenic chryseobacteria has received little attention. Concomitant with the apparent emergence of fish chryseobacteriosis, which is exacerbated by the bacterium's constitutive resistance to a wide spectrum of antibiotics ...

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 85%

Polyphasic characterization reveals the presence of novel fish-associated Chryseobacterium spp. in the Great Lakes of North America

Loch¹,

Faisal

2015

Dis. Aquat. Org.

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“…Such cases include; Chryseobacterium indoligenes (Bernardet et al 2005;Pridgeon et al 2013), Plesiomonas shigelloides (Cruz et al 1986;Nisha et al 2014), Pseudomonas aeruginosa (Thomas et al 2014), Citrobacter spp. (Jeremic et al 2003), Serratia marcescens (Baya et al 1992), Klebsiella spp.…”

Section: Discussionmentioning

confidence: 99%

Occurrence and antibiotic susceptibility of fish bacteria isolated from Oreochromis niloticus (Nile tilapia) and Clarias gariepinus (African catfish) in Uganda

et al. 2018

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The intention of this study was to identify the bacterial pathogens infecting Oreochromis niloticus (Nile tilapia) and Clarias gariepinus (African catfish), and to establish the antibiotic susceptibility of fish bacteria in Uganda. A total of 288 fish samples from 40 fish farms (ponds, cages, and tanks) and 8 wild water sites were aseptically collected and bacteria isolated from the head kidney, liver, brain and spleen. The isolates were identified by their morphological characteristics, conventional biochemical tests and Analytical Profile Index test kits. Antibiotic susceptibility of selected bacteria was determined by the Kirby-Bauer disc diffusion method. The following well-known fish pathogens were identified at a farm prevalence of; Aeromonas hydrophila (43.8%), Aeromonas sobria (20.8%), Edwardsiella tarda (8.3%), Flavobacterium spp. (4.2%) and Streptococcus spp. (6.3%). Other bacteria with varying significance as fish pathogens were also identified including Plesiomonas shigelloides (25.0%), Chryseobacterium indoligenes (12.5%), Pseudomonas fluorescens (10.4%), Pseudomonas aeruginosa (4.2%), Pseudomonas stutzeri (2.1%), Vibrio cholerae (10.4%), Proteus spp. (6.3%), Citrobacter spp. (4.2%), Klebsiella spp. (4.2%) Serratia marcescens (4.2%), Burkholderia cepacia (2.1%), Comamonas testosteroni (8.3%) and Ralstonia picketti (2.1%). Aeromonas spp., Edwardsiella tarda and Streptococcus spp. were commonly isolated from diseased fish. Aeromonas spp. (n = 82) and Plesiomonas shigelloides (n = 73) were evaluated for antibiotic susceptibility. All isolates tested were susceptible to at-least ten (10) of the fourteen antibiotics evaluated. High levels of resistance were however expressed by all isolates to penicillin, oxacillin and ampicillin. This observed resistance is most probably intrinsic to those bacteria, suggesting minimal levels of acquired antibiotic resistance in fish bacteria from the study area. To our knowledge, this is the first study to establish the occurrence of several bacteria species infecting fish; and to determine antibiotic susceptibility of fish bacteria in Uganda. The current study provides baseline information for future reference and fish disease management in the country.

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“…Chryseobacterium indologenes has also been found on Pangasius fish sampled during processing in Vietnam (Tong ) and frozen Pangasius exported to Denmark (Noor Uddin et al 2013). Chryseobacterium indologenes has been isolated from diseased yellow perch (Pridgeon et al 2013) whilst Chryseobacterium spp. are known to be widely distributed in the environment and soil (Benmalek et al 2010), and fresh water Park et al 2008).…”

Section: Microbiota Of Frozen Pangasius Products Marketed In Belgiummentioning

confidence: 99%

Microbiota of frozen Vietnamese catfish (Pangasius hypophthalmus) marketed in Belgium

et al. 2016

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Background: Vietnamese catfish (Pangasius hypophthalmus) is highly appreciated in many European countries, the U.S., Canada, Japan etc. This paper presents an overview of the microbiota of frozen Vietnamese catfish products marketed in Belgium. Samples of Pangasius steaks, portions and fillets from six brands were collected from supermarkets located in Ghent, Belgium. Results: The total psychrotrophic and mesophilic aerobic counts of the samples evaluated from each brand did not differ significantly (p > 0.05) and ranged from 3.8-5.2 log CFU/g and 3.8-4.8 log CFU/g, respectively. Lactic acid bacteria counts varied from 2.2 to 4.1 log CFU/g while the counts of presumptive Enterobacteriaceae ranged from 1.6 to 3.8 log CFU/g. A total of 132 isolates were collected from the plates used to enumerate the microbial parameters mentioned above. Fourteen different genera and 18 different species were identified by means of 16S rRNA gene sequencing. The most prevalent genera of Lactococcus (31.2 %), Staphylococcus (11.7 %), Serratia (10.4 %), Acinetobacter (9.1 %), Enterococcus (7.8 %) and Pseudomonas spp. (6.5 %) were identified by means of 16S rRNA gene sequencing. Conclusion:The results obtained provide an overview of the dominant microbiota on frozen Pangasius which is useful for the development of appropriate preservation techniques for thawed Pangasius products.

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Identification and virulence of Chryseobacterium indologenes isolated from diseased yellow perch (Perca flavescens )

Cited by 30 publications

References 19 publications

Polyphasic characterization reveals the presence of novel fish-associated Chryseobacterium spp. in the Great Lakes of North America

Polyphasic characterization reveals the presence of novel fish-associated Chryseobacterium spp. in the Great Lakes of North America

Occurrence and antibiotic susceptibility of fish bacteria isolated from Oreochromis niloticus (Nile tilapia) and Clarias gariepinus (African catfish) in Uganda

Microbiota of frozen Vietnamese catfish (Pangasius hypophthalmus) marketed in Belgium

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