Identification by fluorescence spectroscopy of lactic acid bacteria isolated from a small-scale facility producing traditional dry sausages

Ammor, Salim; Yaakoubi, Kaoutar; Chevallier, Isabelle; Dufour, Eric

doi:10.1016/j.mimet.2004.07.014

Cited by 49 publications

(39 citation statements)

References 22 publications

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“…In addition, several proteins involved in the redox status of the cells were involved in the bile salt response. This prompted us to determine fluctuations of intracellular pools of NADH and FAD ϩ , whose fluorescence could be monitored under in vivo conditions (2). Changes in the redox ratio, calculated from FAD and NADH fluorescence-related signals (58), reflect changes in the balance between oxidized and reduced forms and hence fluctuation in the redox status of cells, which is dependent on the metabolic reactions occurring at a given time.…”

Section: Resultsmentioning

confidence: 99%

“…The fluorescence properties of buffered cell suspensions (OD 600 , 0.6) in 50 mM Tris-HCl buffer, pH 7.0, were monitored in an Eclipse fluorescence spectophotometer (Varian, Inc., Palo Alto, CA). The intensity values corresponding to NADH were calculated from the 413-nm emission at a ex of 316 nm, whereas for flavin adenine dinucleotide (FAD) the intensity values were calculated from the 436-nm emission at a ex of 380 nm, as described by Ammor et al (2). The redox ratio was deduced from the NADH-and FAD-related signals using the equation redox ratio ϭ FAD intensity/(FAD intensity ϩ NADH intensity) (27).…”

Section: Methodsmentioning

confidence: 99%

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Adaptation and Response of Bifidobacterium animalis subsp. lactis to Bile: a Proteomic and Physiological Approach

Sánchez

Champomier‐Vergès²,

Stuer‐Lauridsen

et al. 2007

Appl Environ Microbiol

115

113

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Bile salts are natural detergents that facilitate the digestion and absorption of the hydrophobic components of the diet. However, their amphiphilic nature makes them very inhibitory for bacteria and strongly influences bacterial survival in the gastrointestinal tract. Adaptation to and tolerance of bile stress is therefore crucial for the persistence of bacteria in the human colonic niche. Bifidobacterium animalis subsp. lactis, a probiotic bacterium with documented health benefits, is applied largely in fermented dairy products. In this study, the effect of bile salts on proteomes of B. animalis subsp. lactis IPLA 4549 and its bile-resistant derivative B. animalis subsp. lactis 4549dOx was analyzed, leading to the identification of proteins which may represent the targets of bile salt response and adaptation in B. animalis subsp. lactis. The comparison of the wild-type and the bile-resistant strain responses allowed us to hypothesize about the resistance mechanisms acquired by the derivative resistant strain and about the bile salt response in B. animalis subsp. lactis. In addition, significant differences in the levels of metabolic end products of the bifid shunt and in the redox status of the cells were also detected, which correlate with some differences observed between the proteomes. These results indicate that adaptation and response to bile in B. animalis subsp. lactis involve several physiological mechanisms that are jointly dedicated to reduce the deleterious impact of bile on the cell's physiology.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Adaptation and Response of Bifidobacterium animalis subsp. lactis to Bile: a Proteomic and Physiological Approach

Sánchez

Champomier‐Vergès²,

Stuer‐Lauridsen

et al. 2007

Appl Environ Microbiol

115

113

View full text Add to dashboard Cite

show abstract

“…The fluorescence properties of buffered cell suspensions (OD 600 of 0.6) in 50 mM Tris-HCl buffer, pH 7.0, were monitored in an Eclipse fluorescence spectrophotometer (Varian, Inc., Palo Alto, CA). The intensity values corresponding to NADH were calculated from the 413-nm emission at ex ϭ 316 nm, whereas for flavin adenine dinucleotide (FAD) the intensity values were calculated from the 436-nm emission at ex ϭ 380 nm, according to the work of Ammor et al (1). The redox ratio was deduced from the NADH-and FAD-related signals using the equation redox ratio ϭ FAD intensity /(FAD intensity ϩ NADH intensity ) (25).…”

Section: Methodsmentioning

confidence: 99%

Low-pH Adaptation and the Acid Tolerance Response of Bifidobacterium longum Biotype longum

Sánchez

Champomier‐Vergès²,

Collado

et al. 2007

Appl Environ Microbiol

180

154

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Bifidobacteria are one of the main microbial inhabitants of the human colon. Usually administered in fermented dairy products as beneficial microorganisms, they have to overcome the acidic pH found in the stomach during the gastrointestinal transit to be able to colonize the lower parts of the intestine. The mechanisms underlying acid response and adaptation in Bifidobacterium longum biotype longum NCIMB 8809 and its acid-pH-resistant mutant B. longum biotype longum 8809dpH were studied. Comparison of protein maps, and protein identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis, allowed us to identify nine different proteins whose production largely changed in the mutant strain. Furthermore, the production of 47 proteins was modulated by pH in one or both strains. These included general stress response chaperones and proteins involved in transcription and translation as well as in carbohydrate and nitrogen metabolism, among others. Significant differences in the levels of metabolic end products and in the redox status of the cells were also detected between the wild-type strain and its acid-pHresistant mutant in response to, or as a result of, adaptation to acid. Remarkably, the results of this work indicated that adaptation and response to low pH in B. longum biotype longum involve changes in the glycolytic flux and in the ability to regulate the internal pH. These changes were accompanied by a higher content of ammonium in the cytoplasm, likely coming from amino acid deamination, and a decrease of the bile salt hydrolase activity.

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“…The emission spectrum Boubellouta and Dufour (2008), Dufour and Riaublanc (1997), Dufour et al (2000), Hammami et al (2010), Herbert et al (1999Herbert et al ( , 2000, Dufour (2003, 2006), Karoui et al 2004a, b, 2005a, b, c, 2006b, e, 2007c, Kulmyrzaev et al (2005 Baunsgaard et al (2000a, b), Bro (1999), Karoui et al (2007a, Munck et al (1998), Nørgaard (1995, Ruoff et al (2006) Noh and Lu (2007), Seiden et al (1996) Ammor et al (2004, Dufour (2002, 2004), Leriche et al (2004), Tourkya et al (2009 Collagen Egelandsdal et al (1996Egelandsdal et al (...…”

Section: Emission Spectrummentioning

confidence: 99%

Fluorescence Spectroscopy Measurement for Quality Assessment of Food Systems—a Review

Karoui

Blecker

2010

Food Bioprocess Technol

309

129

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The present review gives an overview of the use of fluorescence spectroscopy (i.e., conventional, excitationemission matrix, and synchronous fluorescence) for determining changes in food products and their quality during technological process and storage. From the present review, it was shown that fluorescence spectroscopy is able to determine several properties (functional, composition, nutritional) without the use of chemical reagents. This is due to the use of chemometric tools (descriptive and predictive methods). The review focuses on the use of fluorescence spectroscopy for the determination of the quality of animal (i.e., dairy, meat, fish, and egg) and vegetable (oils, cereal, sugar, fruit, and vegetable) products as well as the identification of bacteria of agro-alimentary interest.

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Identification by fluorescence spectroscopy of lactic acid bacteria isolated from a small-scale facility producing traditional dry sausages

Cited by 49 publications

References 22 publications

Adaptation and Response of Bifidobacterium animalis subsp. lactis to Bile: a Proteomic and Physiological Approach

Adaptation and Response of Bifidobacterium animalis subsp. lactis to Bile: a Proteomic and Physiological Approach

Low-pH Adaptation and the Acid Tolerance Response of Bifidobacterium longum Biotype longum

Fluorescence Spectroscopy Measurement for Quality Assessment of Food Systems—a Review

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