Identification by MALDI-TOF MS of Environmental Bacteria with High Potential to Degrade Pyrene

Abstract

<p>An alternative to remove polycyclic aromatic hydrocarbons (PAHs) of the environment is using hydrocarbonoclastic bacteria. The aim of this work was to study the bacterial diversity of indigenous isolates with potential to degrade pyrene. We used MALDI-Biotyper (Bruker Daltonics) as a powerful analytical tool for identification. Bacteria were isolated from surface seawater and marine sediments samples of three sites from the coast of Rosarito Port, B.C., Mexico. Total concentration of PAHs in collected samples was quantified by GC-MS, showed values ranged 0.461-0.525 ng mL<sup>-1</sup> and of 74 -266 ng g<sup>-1</sup> in seawater and sediments samples, respectively. A total of 52 bacteria with capacity to grow in 25 mg L<sup>-1</sup> pyrene as sole carbon and energy source were taxonomically identified and classified by MALDI-Biotyper system by comparing the mass spectra with library and/or to use chemometric tools as Principal Components Analysis (PCA) and Composite Correlation Index (CCI) to evaluate possible differences to isolate level. The identified isolates were represented by three phylogenetic groups: <em>Firmicutes, Actinobacteria and Proteobacteria</em>. Of these isolates, <em>Kocuria</em> strains appear to be excellent candidates to continue PAHs degradation studies.</p>

Identification of Microorganisms from Several Surfaces by MALDI-TOF MS: P. aeruginosa Is Leading in Biofilm Formation

“…2 ) shows a high degree of sequence homology within the pseudomonads, which confuses species identification by analysis of 16S rRNA sequences only. Therefore, we used MALDI-TOF-MS, a method which can easily discriminate between P. putida and P. aeruginosa [ 75 ]. Alternatively, this discrepancy may be explained by differences in cultivation conditions, particularly, temperature, growth medium, and growth rate.…”

Influence of environmental settings in geographically distinct ports on the protein profiles of cultivable bacteria using MALDI–TOF mass spectrometry

“…Silva‐Jiménez et al. (2018) through MTB reported the presence of Acinetobacter johnsonii , Alcaligenes faecalis , Micrococcus luteus , Brachybacterium faecium in the sediment of Rosarito Port, Mexico. These species have demonstrated hydrocarbon‐degrading capability.…”

“…Many of Pseudomonas spp. have the ability to produce bioactive substances, enzymes, biosurfactants and have been widely used in the bioremediation process and to control plant diseases (Silva‐Jiménez et al., 2018). The dominance of Pseudomonas spp.…”

Oil-degrading bacterial consortium from Gulf of Mexico designed by a factorial method, reveals stable population dynamics

“…Bacterial isolates were plated in LB agar (Bertani, 2004) or EDM agar (Rosas-Dıáz et al, 2021). Three additional strains were isolated from sediment samples collected in the coastal area of Rosarito Port, Baja California, in the MPO (Silva-Jimeńez et al, 2018) (Table 1). These strains were isolated using Bushnell Haas (BH) broth supplemented with 25 mg•L -1 pyrene or phenanthrene.…”

“…From marine sediment samples collected in an area of 200,000 km 2 , and using high throughput sequencing, we have identified at least 450 genera belonging to the bacterial baseline (Godoy-Lozano et al, 2018;Raggi et al, 2020). In addition to this effort, we have isolated some bacterial strains from the GoM (Muriel-Millań et al, 2019;Muriel-Millań et al, 2021;Rodrıǵuez-Salazar et al, 2021;Rosas-Dıáz et al, 2021;Rojas-Vargas et al, 2022) and from the Northwest region of the Mexican Pacific Ocean (MPO) (Silva-Jimeńez et al, 2018). These strains were isolated from water column and sediment samples collected at locations where crude oil was reported to be present; as such, we expect to find HC degraders among them, which could potentially be used to design a promising bacterial consortium for HC bioremediation in seawater.…”