Identification by Subtractive Hybridization of a Novel Insertion Sequence Specific for Virulent Strains of
            <i>Porphyromonas gingivalis</i>

Sawada, Kayo; Kokeguchi, Susumu; Hongyo, Hiroshi; Sawada, Satoko; Miyamoto, Manabu; Maeda, Hiroshi; Nishimura, Fusanori; Takashiba, Shogo; Murayama, Yoji

doi:10.1128/iai.68.9.5469a-5469a.2000

Cited by 7 publications

(13 citation statements)

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“…There are multiple clonal structures and ribotypes of P. gingivalis, and the pathogenicity of each clone or type has not been clearly defined. 40,41 The lack of agreement between our work and the previous studies demonstrates that further detailed experiments are required to clarify the role of different types or clones of P. gingivalis in the development of periodontal disease.…”

Section: Discussionmentioning

confidence: 49%

IgG Antibody Levels to Porphyromonas gingivalis and Clinical Measures in Children

Donley

Badovinac

Sapir

et al. 2004

Journal of Periodontology

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Section: Discussionmentioning

confidence: 49%

IgG Antibody Levels to Porphyromonas gingivalis and Clinical Measures in Children

Donley

Badovinac

Sapir

et al. 2004

Journal of Periodontology

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“…Even if the function of the different regions is unclear, the nucleotide sequences can serve as diagnostic markers for DNA‐based detection (e.g. [5–8]).…”

Section: Introductionmentioning

confidence: 99%

Use of subtractive hybridization for comprehensive surveys of prokaryotic genome differences

Agron

Macht

Radnedge

et al. 2002

FEMS Microbiology Letters

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Comparative bacterial genomics shows that even different isolates of the same bacterial species can vary significantly in gene content. An effective means to survey differences across whole genomes would be highly advantageous for understanding this variation. Here we show that suppression subtractive hybridization (SSH) provides high, representative coverage of regions that differ between similar genomes. Using Helicobacter pylori strains 26695 and J99 as a model, SSH identified approximately 95% of the unique open reading frames in each strain, showing that the approach is effective. Furthermore, combining data from parallel SSH experiments using different restriction enzymes significantly increased coverage compared to using a single enzyme. These results suggest a powerful approach for assessing genome differences among closely related strains when one member of the group has been completely sequenced.

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“…In this regard, it is interesting to note that Sawada et al. suggested that the presence of IS Pg4 (designated IS 1598 in their study) may be a specific marker for virulent strains (25). This hypothesis was based on the finding that this element was present in strains W83, W50, and 381, which are able to form abscesses in animal models, and was absent in avirulent strains including ATCC 33277.…”

Section: Discussionmentioning

confidence: 90%

“…IS elements are usually 800–2500 bp in length, contain an open reading frame encoding a transposase, have terminal inverted repeats, and are bounded by characteristic short direct repeats (21). Five IS elements have been described for P. gingivalis (22–26). These IS elements have been recently designated by a standard nomenclature as IS Pg1–5 (22).…”

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confidence: 99%

“…An additional five IS elements designated IS Pg6–10 have been found in P. gingivalis strain W83 (personal communication, Floyd Dewhirst). The restriction fragment length polymorphism (RFLP) patterns observed on Southern blots probed with IS Pg1 , 3 , 4 , and 5 have been reported (22–25) for a limited number of primarily laboratory strains. We have now extended this analysis to include IS Pg1–7 and fresh clinical isolates.…”

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confidence: 99%

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The genetic relatedness of Porphyromonas gingivalis clinical and laboratory strains assessed by analysis of insertion sequence (IS) element distribution

Califano

Arimoto

Kitten

2003

J of Periodontal Research

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Transposition and recombination between IS elements are not sufficiently pervasive to obscure strain relationships, though this does not preclude the possibility that such events play an important role in allowing P. gingivalis to adapt to new environments. Given the level of genetic diversity observed, it may be especially important to examine genetically diverse strains when drawing conclusions based on the W83 P. gingivalis genomic database.

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Identification by Subtractive Hybridization of a Novel Insertion Sequence Specific for Virulent Strains of Porphyromonas gingivalis

Cited by 7 publications

References 0 publications

IgG Antibody Levels to Porphyromonas gingivalis and Clinical Measures in Children

IgG Antibody Levels to Porphyromonas gingivalis and Clinical Measures in Children

Use of subtractive hybridization for comprehensive surveys of prokaryotic genome differences

The genetic relatedness of Porphyromonas gingivalis clinical and laboratory strains assessed by analysis of insertion sequence (IS) element distribution

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