Identification, Characterization, and Expression Levels of Putative Adhesive Proteins From the Tube-Dwelling Polychaete <i>Sabellaria alveolata</i>

Becker, Pierre; Lambert, Aurélie; Lejeune, Aude; Lanterbecq, Déborah; Flammang, Patrick

doi:10.1086/bblv223n2p217

Cited by 35 publications

(30 citation statements)

References 27 publications

(55 reference statements)

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“…To test for the possible presence of L-DOPA in the cement, pieces were stained according to the protocol of Arnow (1937). Samples from the tube-dwelling polychaete Sabellaria alveolata were used as a positive control (Becker et al 2012).…”

Section: Staining Methodsmentioning

confidence: 99%

Biochemical analyses of the cement float of the goose barnacleDosima fascicularis– a preliminary study

et al. 2014

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The goose barnacle Dosima fascicularis produces an excessive amount of adhesive (cement), which has a double function, being used for attachment to various substrata and also as a float (buoy). This paper focuses on the chemical composition of the cement, which has a water content of 92%. Scanning electron microscopy with EDX was used to measure the organic elements C, O and N in the foam-like cement. Vibrational spectroscopy (FTIR, Raman) provided further information about the overall secondary structure, which tended towards a β-sheet. Disulphide bonds could not be detected by Raman spectroscopy. The cystine, methionine, histidine and tryptophan contents were each below 1% in the cement. Analyses of the cement revealed a protein content of 84% and a total carbohydrate content of 1.5% in the dry cement. The amino acid composition, 1D/2D-PAGE and MS/MS sequence analysis revealed a de novo set of peptides/proteins with low homologies with other proteins such as the barnacle cement proteins, largely with an acidic pI between 3.5 and 6.0. The biochemical composition of the cement of D. fascicularis is similar to that of other barnacles, but it shows interesting variations.

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Section: Staining Methodsmentioning

confidence: 99%

Biochemical analyses of the cement float of the goose barnacleDosima fascicularis– a preliminary study

et al. 2014

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“…Furthermore, L-3,4-dihydroxyphenylalanine [L-DOPA; Arnow staining (Arnow, 1937)] were performed on tissue and isolated mucus samples. Samples of the tube-dwelling polychaete Sabellaria alveolata Linnaeus, 1767 were used as positive control (Becker, Lambert, Lejeune, Lanterbecq, & Flammang, 2012). All staining processes listed above took place at room temperature.…”

Section: Mucus Chemistrymentioning

confidence: 99%

The structure of the cutaneous pedal glands in the banded snail Cepaea hortensis (Müller, 1774)

Byern

Cyran

Klepal

et al. 2017

Journal of Morphology

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Although gastropods have been crawling through the ocean and on the land for 60 million years, we still know very little about the sticky mucus produced in their foot. Most research has been focused on marine species in particular and, to a lesser extent, on the well-known terrestrial species Arion vulgaris and Cornu aspersum. Within this study, we aim to characterize the foot anatomy of a smaller representative of the family Helicidae, the banded snail Cepaea hortensis. We are particularly interested in the microanatomy of the foot glands, their position, and the histochemical nature of their secretory content. Characterization of the dorsal foot region of Cepaea hortensis reveals four glands, differing in their size and in the granules produced. Histochemically, three of them react positively for sugars (PAS staining and lectin affinity tests for mannose, glucose and N-acetyl-d-glucosamine) and acidic proteins (positive Alcian blue and Toluidine blue staining), indicating the presence of acidic glycosaminoglycans. The fourth gland type does not react to any of these dyes. The ventral pedal region includes two different gland types, which are positive for the presence of acidic glycoproteins, with a lectin affinity for mannose only. A comparison with Helix pomatia indicates differences regarding the number of glands and their contents. In Helix, only three gland types are described in the dorsal region of the foot, which show a similar granular appearance but nevertheless differ in their chemical composition. Congruently, there are two gland types in the ventral region in both species, whereas in Helix an additional sugar moiety is found. This raises the question whether these differences between the pedal glandular systems of both helicid species are the result of protection or size-related adaptations, as they occur in the same habitat.

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“…Samples from the tube-dwelling polychaete Sabellaria alveolata were used as positive control (Becker et al, 2012).…”

Section: Histology and Histochemistrymentioning

confidence: 99%

Morphological characterization of the glandular system in the salamander Plethodon shermani (Caudata, Plethodontidae)

Byern

Dicke

Heiss

et al. 2015

Zoology

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Identification, Characterization, and Expression Levels of Putative Adhesive Proteins From the Tube-Dwelling Polychaete Sabellaria alveolata

Cited by 35 publications

References 27 publications

Biochemical analyses of the cement float of the goose barnacleDosima fascicularis– a preliminary study

Biochemical analyses of the cement float of the goose barnacleDosima fascicularis– a preliminary study

The structure of the cutaneous pedal glands in the banded snail Cepaea hortensis (Müller, 1774)

Morphological characterization of the glandular system in the salamander Plethodon shermani (Caudata, Plethodontidae)

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