Identification, Characterization and Partial Purification of Acid Phosphatase from Cotyledons of Psoralea corylifolia L.

Singh, Satendra; Luthra, Pratibha Mehta

doi:10.3923/ajpp.2011.228.241

Cited by 6 publications

(9 citation statements)

References 25 publications

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“…The second most common hydrolytic enzyme is acid phosphatase, which catalyses the hydrolysis of phosphate esters at an acidic pH and supplies soluble forms of phosphate to growing parts of the plant (Singh and Luthra, 2011). Under salinity stress, a reduction in the activity of acid phosphatase has been observed in lettuce seedlings (Nasri et al , 2012), while exogenous application of gibberellic acid increased the activity of acid phosphatase during seed germination of rye and barley (Centeno et al , 2001).…”

Section: Discussionmentioning

confidence: 99%

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Biochemical changes associated with gibberellic acid-like activity of smoke-water, karrikinolide and vermicompost leachate during seedling development ofPhaseolus vulgarisL.

2014

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Smoke-water (SW), karrikinolide (KAR1) and vermicompost leachate (VCL) have been reported to possess gibberellic acid-like activity. The effects of these plant growth-promoting substances (PGPSs) on biochemical changes occurring during seed germination of Phaseolus vulgaris were assessed. Seeds were incubated/germinated under dark conditions in water (control) or with different concentrations of SW, KAR1, VCL and gibberellic acid (GA3) for 7 d. The maximum seedling fresh weight (1.863 g) was recorded for the SW (1:750 v/v) treatment. The longest seedling axes (9.9 cm) and the highest number of adventitious roots (16.3) were recorded for VCL-treated seedlings (1:10 and 1:5 v/v, respectively). Analysis of two important hydrolytic enzymes, acid phosphatase and alpha-amylase, showed maximum activity [1856 nkat mg− 1 and 3.225 mg min− 1(g FW)− 1, respectively] in the seeds incubated with 10− 8M KAR1. In all the treated seedlings, proline content was significantly reduced [43.67 μg (g FW)− 1; VCL (1:20 v/v)] in comparison to the control [87 μg (g FW)− 1] but there was an increase in amino acids with some concentrations of PGPSs. The tested PGPSs significantly influenced various biochemical parameters that play a significant role in seed germination and plant growth. This study indicates that PGPSs may act via stress-relieving biochemical pathways during seed germination.

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Section: Discussionmentioning

confidence: 99%

“…The acid phosphatase assay was carried out at 37°C (Singh and Luthra, 2011). Crude enzyme extract (15 μl) was added to the sodium acetate buffer (0.1 M, 985 μl; pH 5.0) containing the substrate para -nitrophenyl phosphate ( p- NPP; 2 mM).…”

Section: Methodsmentioning

confidence: 99%

Biochemical changes associated with gibberellic acid-like activity of smoke-water, karrikinolide and vermicompost leachate during seedling development ofPhaseolus vulgarisL.

2014

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“…Phosphate is one of the products of the APase catalyzed reaction. There are reports of inhibition of APase activity in the presence of molybdate (Sugiura et al, 1981;Kruzel & Morawiecka, 1982;Ching et al, 1987;Miernyk, 1992;Pasqualini et al, 1992;Gellatly et al, 1994;Olczak et al, 1997;Tso & Chen, 1997;Cashikar et al, 1997;Granjeiro et al, 1999;Ferreira et al, 2000;Turner & Plaxton, 2001;Coello, 2002;Senna et al, 2006;Singh & Luthra, 2011;Khan et al, 2016). Phosphate and molybdate are most often inhibitors of APase did not affect the APase from tomato leaves (Tanaka et al, 1990).…”

Section: B Effect Of Anionsmentioning

confidence: 97%

“…1987;Gellatly et al, 1994;Olczak et al, 1997;Cashikar et al, 1997;Granjeiro et al, 1999;Roknabadi et al, 1999;Turner & Plaxton, 2001;Koffi et al, 2010;Asaduzzaman et al, 2011;Anand & Srivastava, 2013;Khan et al, 2016). Inhibition in the presence of Cu 2+ ions got observed in some APases (Sugiura et al, 1981;Waymack & Van Etten, 1991;Tso & Chen, 1997;Granjeiro et al, 1999;Yenigun & Guvenilir, 2003;Garcia et al, 2004;Singh & Luthra, 2011;Anand & Srivastava, 2013;Chafik, 2020).…”

Section: A Effect Of Cationsmentioning

confidence: 99%

“…Molybdate and phosphate anions proved stronger inhibitors to the vanadate anion. Inhibition in the enzyme activity got reported in the presence of phosphate in different APases (Sugiura et al, 1981;Ching et al, 1987;Gellatly et al, 1994;Olczak et al, 1997;Granjeiro et al, 1999;Turner & Plaxton, 2001;Cirkovic et al, 2002;Singh & Luthra, 2011). Phosphate is one of the products of the APase catalyzed reaction.…”

Section: B Effect Of Anionsmentioning

confidence: 99%

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Rice Bean (Vigna umbellata Thunb.) Acid Phosphatase: Effect of Metal Ions and Metabolites

Nongpiur,

Ambasht

2022

JSR

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Rice bean acid phosphatase dialyzed against ethylenediaminetetracetic acid (1 mM), yielded no loss in activity at 0.5 mM p-nitrophenylphosphate. It had a mild activating effect at 0.1 mM p-nitrophenylphosphate. Divalent cations belonging to group II of Periodic Table like Mg2+, Ca2+, Sr2+, and Ba2+ at 0.5 mM concentration p-nitrophenylphosphate did not affect the enzyme activity. At 0.1 mM p-nitrophenylphosphate, however, only Mg2+ ions showed little inhibition. The transition metal ions viz. Zn2+ , Cu2+, Co2+, Mn2+ and Fe3+ showed inhibition at both pnitrophenylphosphate concentrations. Na+ , K+ , and NH4 + ions did not influence the enzyme activity, but Li+ inhibited the activity at 0.1 and 0.5 mM p-nitrophenylphosphate concentrations, respectively. Molybdate and phosphate anions proved to be strong inhibitors, but vanadate, a moderate inhibitor. Tartrate anion did not exhibit an effect on rice bean acid phosphatase. Sugars, plant hormones, medicines, vitamins, and amino acids at 1.0 mM concentration did not affect the enzyme activity. NADH exhibited the maximum activation, followed by citric acid, isocitrate, and oxaloacetate. Caffeine showed activation in the enzyme activity. Phosphate esters (1-Naphthylphosphate, phenylphosphate, PEP, and ADP) exhibited competitive inhibition when pnitrophenylphosphate was used as a substrate. Non-ionic detergents Triton X-100 and Tween-20 brought activation to the enzyme. The ionic detergent SDS brought complete loss to the enzyme activity. Dithiothreitol brought inhibition to the enzyme activity. The presence of β-mercaptoethanol did not influence the enzyme activity.

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Purification and partial characterization of acid phosphatase from rice bean (Vigna umbellata Thunb.)

et al. 2021

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Identification, Characterization and Partial Purification of Acid Phosphatase from Cotyledons of Psoralea corylifolia L.

Cited by 6 publications

References 25 publications

Biochemical changes associated with gibberellic acid-like activity of smoke-water, karrikinolide and vermicompost leachate during seedling development ofPhaseolus vulgarisL.

Biochemical changes associated with gibberellic acid-like activity of smoke-water, karrikinolide and vermicompost leachate during seedling development ofPhaseolus vulgarisL.

Rice Bean (Vigna umbellata Thunb.) Acid Phosphatase: Effect of Metal Ions and Metabolites

Purification and partial characterization of acid phosphatase from rice bean (Vigna umbellata Thunb.)

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