Identification, characterization and regulation of two secreted polygalacturonases of the emerging rice pathogen Burkholderia glumae

Degrassi, Giuliano; Devescovi, Giulia; Kim, Jinwoo; Hwang, Ingyu; Venturi, Vittorio

doi:10.1111/j.1574-6941.2008.00516.x

Cited by 26 publications

(17 citation statements)

References 45 publications

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“…The lower than 66 kDa PGaseLg apparent molecular weight determined by Superose 12 HR gel filtration chromatography is indicative that the enzyme was isolated as a monomer. These results are in agreement with published results for polygalacturonases from Burkholderiaglumae [37] and T. harzianum [32].…”

Section: Purification Of Pgaselgsupporting

confidence: 83%

Characterization of an Exopolygalacturonase from Leucoagaricus gongylophorus, the Symbiotic Fungus of Atta sexdens

Adalberto¹,

Golfeto²,

Moreira³

et al. 2016

AER

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The present study aimed to purify and characterize one polygalacturonase from L. gongylophorus (PGaseLg), the symbiotic fungus of Atta sexdens. The enzyme was isolated by salting out of crude extract followed by two chromatographic steps. PGaseLG was identified with MS analysis and molecular exclusion chromatography revealed the monomeric nature of a protein with an estimated molecular weight of about 39 kDa. PGaseLg has an optimum temperature of 60˚C and optimum pH activity at 5.0. Using polygalacturonate as a substrate, the calculations of KM, Vmax and kcat were 0.65 mg•mL −1 , 1800 μmol•min −1 •mg −1 and 35.97 s −1 , respectively. The enzyme was stable for more than 3 h at 50˚C at pH 5.0; otherwise, at lower or higher pH values, the PGaseLg was less stable. The influence of several metals, EDTA and β-mercaptoethanol on enzyme activity was also determined. Thin layer chromatography (TLC) analyses indicated that PGaseLg is an exopolygalacturonase.

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Section: Purification Of Pgaselgsupporting

confidence: 83%

Characterization of an Exopolygalacturonase from Leucoagaricus gongylophorus, the Symbiotic Fungus of Atta sexdens

Adalberto¹,

Golfeto²,

Moreira³

et al. 2016

AER

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“…Toxoflavin and lipase production as well as the bacterial motility mediated by flagella are controlled by a quorum-sensing system composed of a LuxI-family acyl-homoserine lactone (AHL) synthase, TofI, and a LuxR-family AHL receptor, TofR [9], [11], [12]. Additional virulence factors known to contribute to the full virulence of B. glumae include the PehA and PehB polygalacturonases [13], the KatG catalase [14], and the Hrp type III secretion system (T3SS) [15].…”

Section: Introductionmentioning

confidence: 99%

Diversities in Virulence, Antifungal Activity, Pigmentation and DNA Fingerprint among Strains of Burkholderia glumae

et al. 2012

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Burkholderia glumae is the primary causal agent of bacterial panicle blight of rice. In this study, 11 naturally avirulent and nine virulent strains of B. glumae native to the southern United States were characterized in terms of virulence in rice and onion, toxofalvin production, antifungal activity, pigmentation and genomic structure. Virulence of B. glumae strains on rice panicles was highly correlated to virulence on onion bulb scales, suggesting that onion bulb can be a convenient alternative host system to efficiently determine the virulence of B. glumae strains. Production of toxoflavin, the phytotoxin that functions as a major virulence factor, was closely associated with the virulence phenotypes of B. glumae strains in rice. Some strains of B. glumae showed various levels of antifungal activity against Rhizoctonia solani, the causal agent of sheath blight, and pigmentation phenotypes on casamino acid-peptone-glucose (CPG) agar plates regardless of their virulence traits. Purple and yellow-green pigments were partially purified from a pigmenting strain of B. glumae, 411gr-6, and the purple pigment fraction showed a strong antifungal activity against Collectotrichum orbiculare. Genetic variations were detected among the B. glumae strains from DNA fingerprinting analyses by repetitive element sequence-based PCR (rep-PCR) for BOX-A1R-based repetitive extragenic palindromic (BOX) or enterobacterial repetitive intergenic consensus (ERIC) sequences of bacteria; and close genetic relatedness among virulent but pigment-deficient strains were revealed by clustering analyses of DNA fingerprints from BOX-and ERIC-PCR.

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“…Although it is not classified as a human pathogen, a single case of the isolation of B. glumae from a clinical sample was reported (10), indicating that at least some strains of this pathogen may be associated with opportunistic infections in humans. The phytopathogenicity of B. glumae is caused by multiple factors, including the biosynthesis of toxoflavin (11,12); motility (13); the secretion of virulence factors by a type III secretion system (T3SS) (14); and the production of lipase (15), catalase (16), and pectate lyase (17). Since the expression of many of the respective genes is controlled by AHLs, QS plays a major role during plant infection processes as well (11,18).…”

mentioning

confidence: 99%

Genome-Wide RNA Sequencing Analysis of Quorum Sensing-Controlled Regulons in the Plant-Associated Burkholderia glumae PG1 Strain

Gao

Krysciak

Petersen

et al. 2015

Appl Environ Microbiol

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dBurkholderia glumae PG1 is a soil-associated motile plant-pathogenic bacterium possessing a cell density-dependent regulation system called quorum sensing (QS). Its genome contains three genes, here designated bgaI1 to bgaI3, encoding distinct autoinducer-1 (AI-1) synthases, which are capable of synthesizing QS signaling molecules. Here, we report on the construction of B. glumae PG1 ⌬bgaI1, ⌬bgaI2, and ⌬bgaI3 mutants, their phenotypic characterization, and genome-wide transcriptome analysis using RNA sequencing (RNA-seq) technology. Knockout of each of these bgaI genes resulted in strongly decreased motility, reduced extracellular lipase activity, a reduced ability to cause plant tissue maceration, and decreased pathogenicity. RNA-seq analysis of all three B. glumae PG1 AI-1 synthase mutants performed in the transition from exponential to stationary growth phase revealed differential expression of a significant number of predicted genes. In comparison with the levels of gene expression by wild-type strain B. glumae PG1, 481 genes were differentially expressed in the ⌬bgaI1 mutant, 213 were differentially expressed in the ⌬bgaI2 mutant, and 367 were differentially expressed in the ⌬bgaI3 mutant. Interestingly, only a minor set of 78 genes was coregulated in all three mutants. The majority of the QS-regulated genes were linked to metabolic activities, and the most pronounced regulation was observed for genes involved in rhamnolipid and Flp pilus biosynthesis and the type VI secretion system and genes linked to a clustered regularly interspaced short palindromic repeat (CRISPR)-cas gene cluster. Q uorum sensing (QS) is a cell density-dependent gene regulation system in bacteria (1) in which the population density is sensed through the accumulation of bacterially produced signaling molecules called autoinducers (AIs). This cell-to-cell signaling process allows the microbial population to synchronize group behavior and alter its gene expression accordingly. QS is involved in a wide array of regulatory circuits, among which are pathogenicity, secretion of extracellular proteins, secondary metabolite production, and others (2). Key QS signaling molecules in many Gram-negative bacteria are N-acyl-homoserine lactones (AHLs) (3-5), synthesized mainly through LuxI homologs (EC 2.3.1.184) using S-adenosylmethionine (SAM), and an acyl-acyl carrier protein (acyl-ACP) from the fatty acid biosynthesis pathway (6). LuxR-type receptor/regulator proteins are involved in AHL signal perception. Together with LuxR, other proteins may be part of this regulatory circuit.The motile, rod-shaped Gram-negative soil bacterium Burkholderia glumae is considered to be a seed-borne pathogen that causes panicle blight of rice (7). B. glumae has also been reported to infect other plant species, like tomato, sunflower, and pepper (8, 9). Although it is not classified as a human pathogen, a single case of the isolation of B. glumae from a clinical sample was reported (10), indicating that at least some strains of this pathogen may be associated w...

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Identification, characterization and regulation of two secreted polygalacturonases of the emerging rice pathogen Burkholderia glumae

Cited by 26 publications

References 45 publications

Characterization of an Exopolygalacturonase from <i>Leucoagaricus gongylophorus</i>, the Symbiotic Fungus of <i>Atta sexdens</i>

Characterization of an Exopolygalacturonase from <i>Leucoagaricus gongylophorus</i>, the Symbiotic Fungus of <i>Atta sexdens</i>

Diversities in Virulence, Antifungal Activity, Pigmentation and DNA Fingerprint among Strains of Burkholderia glumae

Genome-Wide RNA Sequencing Analysis of Quorum Sensing-Controlled Regulons in the Plant-Associated Burkholderia glumae PG1 Strain

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Identification, characterization and regulation of two secreted polygalacturonases of the emerging rice pathogen Burkholderia glumae

Cited by 26 publications

References 45 publications

Characterization of an Exopolygalacturonase from &lt;i&gt;Leucoagaricus gongylophorus&lt;/i&gt;, the Symbiotic Fungus of &lt;i&gt;Atta sexdens&lt;/i&gt;

Characterization of an Exopolygalacturonase from &lt;i&gt;Leucoagaricus gongylophorus&lt;/i&gt;, the Symbiotic Fungus of &lt;i&gt;Atta sexdens&lt;/i&gt;

Diversities in Virulence, Antifungal Activity, Pigmentation and DNA Fingerprint among Strains of Burkholderia glumae

Genome-Wide RNA Sequencing Analysis of Quorum Sensing-Controlled Regulons in the Plant-Associated Burkholderia glumae PG1 Strain

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Characterization of an Exopolygalacturonase from <i>Leucoagaricus gongylophorus</i>, the Symbiotic Fungus of <i>Atta sexdens</i>

Characterization of an Exopolygalacturonase from <i>Leucoagaricus gongylophorus</i>, the Symbiotic Fungus of <i>Atta sexdens</i>