Identification, characterization and structure analysis of a type I ribosome-inactivating protein from Sapium sebiferum (Euphorbiaceae)

Wu, Ying; Mao, Yingji; Jin, Songqing; Hou, Jinyan; Du, Hao; Yang, Minglei; Wu, Lifang

doi:10.1016/j.bbrc.2015.05.089

Cited by 6 publications

(2 citation statements)

References 41 publications

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“…The assay was performed according to the method described by Wu et al ( 29 ) with several revisions. Total RNA was extracted from Sapium sebiferum leaves (collected from the test field at Hefei Institutes of Physical Science) by the method mentioned above.…”

Section: Methodsmentioning

confidence: 99%

Fordin: A novel type I ribosome inactivating protein from Vernicia fordii modulates multiple signaling cascades leading to anti-invasive and pro-apoptotic effects in cancer cells in�vitro

Mao

Chen

et al. 2018

Int J Oncol

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Fordin, which is derived from Vernicia fordii, is a novel type I ribosome inactivating protein (RIP) with RNA N-glycosidase activity. In the present study, fordin was expressed by Escherichia coli and purified using nickel affinity chromatography. Previous studies have demonstrated RIP toxicity in a variety of cancer cell lines. To understand the therapeutic potential of fordin on tumors, the present study investigated the effects of fordin on the viability of several tumor and normal cell lines. The results demonstrated that fordin induced significant cytotoxicity in four cancer cell lines, compared with the normal cell line. Specifically, profound apoptosis and inhibition of cell invasion were observed following fordin exposure in U-2 OS and HepG2 cells; however, the molecular mechanism underlying the action of RIP remains to be fully elucidated. In the present study, it was found that the anticancer effects of fordin were associated with suppression of the nuclear factor (NF)-κB signaling pathway. In U-2 OS and HepG2 cells, fordin inhibited the expression of inhibitor of NF-κB (IκB) kinase, leading to downregulation of the phosphorylation level of IκB, which quelled the nuclear translocation of NF-κB. Fordin also reduced the mRNA and protein levels of NF-κB downstream targets associated with cell apoptosis and metastasis, particularly B-cell lymphoma-2-related protein A1 (Blf-1) and matrix metalloproteinase (MMP)-9. The inactivation of NF-κB and the reduction in the expression levels of Blf-1 and MMP-9 mediated by fordin were also confirmed by co-treatment with lipopolysaccharide or p65 small interfering RNA. These findings suggested a possible mechanism for the fordin-induced effect on tumor cell death and metastasis. The results of the present study demonstrated the multiple anticancer effects of fordin in U-2 OS and HepG2 cells, in part by inhibiting activation of the NF-κB signaling pathway.

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Section: Methodsmentioning

confidence: 99%

Fordin: A novel type I ribosome inactivating protein from Vernicia fordii modulates multiple signaling cascades leading to anti-invasive and pro-apoptotic effects in cancer cells in�vitro

Mao

Chen

et al. 2018

Int J Oncol

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“…Ribosome-inactivating proteins (RIPs), members of N-glycosidases (EC3.2.2.22) and produced by various organisms including bacteria, fungi, and plants, are a diverse group of toxins sharing ribosome inactivating activity. 1,2 Ricin was the rst RIP found in Ricinus communis L. 3 In addition, Shiga and Shiga-like toxins were found in Shigella spp. and Escherichia coli.…”

Section: Introductionmentioning

confidence: 99%

Ribosome-inactivating proteins (RIPs) and their important health promoting property

Wang

et al. 2016

RSC Adv.

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Natural Compounds Extracted from Medicinal Plants and Their Immunomodulatory Activities

Gurjar

Pal

2020

Advanced Structured Materials

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Identification, characterization and structure analysis of a type I ribosome-inactivating protein from Sapium sebiferum (Euphorbiaceae)

Cited by 6 publications

References 41 publications

Fordin: A novel type I ribosome inactivating protein from Vernicia fordii modulates multiple signaling cascades leading to anti-invasive and pro-apoptotic effects in cancer cells in�vitro

Fordin: A novel type I ribosome inactivating protein from Vernicia fordii modulates multiple signaling cascades leading to anti-invasive and pro-apoptotic effects in cancer cells in�vitro

Ribosome-inactivating proteins (RIPs) and their important health promoting property

Natural Compounds Extracted from Medicinal Plants and Their Immunomodulatory Activities

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