Identification, classification, and characterization of AP2/ERF superfamily genes in Masson pine (Pinus massoniana Lamb.)

et al. 2022

Front. Plant Sci.

Ethylene Responsive Factor (ERF) subfamily comprise the largest number of proteins in the plant AP2/ERF superfamily, and have been most extensively studied on the biological functions. Members of this subfamily have been proven to regulate plant resistances to various abiotic stresses, such as drought, salinity, chilling and some other adversities. Under these stresses, ERFs are usually activated by mitogen-activated protein kinase induced phosphorylation or escape from ubiquitin-ligase enzymes, and then form complex with nucleic proteins before binding to cis-element in promoter regions of stress responsive genes. In this review, we will discuss the phylogenetic relationships among the ERF subfamily proteins, summarize molecular mechanism how the transcriptional activity of ERFs been regulated and how ERFs of different subgroup regulate the transcription of stress responsive genes, such as high-affinity K+ transporter gene PalHKT1;2, reactive oxygen species related genes LcLTP, LcPrx, and LcRP, flavonoids synthesis related genes FtF3H and LhMYBSPLATTER, etc. Though increasing researches demonstrate that ERFs are involved in various abiotic stresses, very few interact proteins and target genes of them have been comprehensively annotated. Hence, future research prospects are described on the mechanisms of how stress signals been transited to ERFs and how ERFs regulate the transcriptional expression of stress responsive genes.

Section: Introductionmentioning

confidence: 99%

ERF subfamily transcription factors and their function in plant responses to abiotic stresses

et al. 2022

Front. Plant Sci.

“…The P. massoniana LBD gene family have not been discovered because of its genome’s enormous size. Therefore, using its transcriptome datasets [ 29 ], we were able to identify the LBD family members. By phylogenetic grouping and protein motif structure analysis, the PmLBD family was categorized using the Arabidopsis LBD family classification as a guide [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

“…The PmLBD family was classified by phylogenetic grouping and protein motif structure analysis. In addition, the basic information, physicochemical properties, genetic structure, evolutionary relationships, conserved structural domain features and expression patterns of P. massoniana LBD genes were comprehensively analyzed [ 29 ]. This study lays the foundation for future studies on LBD genes in P. massoniana , and also facilitates future studies on the response of P. massoniana to environmental stresses.…”

Section: Introductionmentioning

confidence: 99%

Identification, Classification and Characterization of LBD Transcription Factor Family Genes in Pinus massoniana

Zhu

et al. 2022

IJMS

Self Cite

Transcription factors (TFs) are a class of proteins that play an important regulatory role in controlling the expression of plant target genes by interacting with downstream regulatory genes. The lateral organ boundary (LOB) structural domain (LBD) genes are a family of genes encoding plant-specific transcription factors that play important roles in regulating plant growth and development, nutrient metabolism, and environmental stresses. However, the LBD gene family has not been systematically identified in Pinus massoniana, one of the most important conifers in southern China. Therefore, in this study, we combined cell biology and bioinformatics approaches to identify the LBD gene family of P. massoniana by systematic gene structure and functional evolutionary analysis. We obtained 47 LBD gene family members, and all PmLBD members can be divided into two subfamilies, (Class I and Class II). By treating the plants with abiotic stress and growth hormone, etc., under qPCR-based analysis, we found that the expression of PmLBD genes was regulated by growth hormone and abiotic stress treatments, and thus this gene family in growth and development may be actively involved in plant growth and development and responses to adversity stress, etc. By subcellular localization analysis, PmLBD is a nuclear protein, and two of the genes, PmLBD44 and PmLBD45, were selected for functional characterization; secondly, yeast self-activation analysis showed that PmLBD44, PmLBD45, PmLBD46 and PmLBD47 had no self-activating activity. This study lays the foundation for an in-depth study of the role of the LBD gene family in other physiological activities of P. massoniana.

“…Previous studies have elucidated the important role of volatile esters in flavor formation in apple fruit. , However, TFs that could directly regulate ester formation have been less frequently reported in apple. Some studies have revealed the roles of various TFs in the biosynthesis of other volatile compounds; for example, terpenoid metabolism is regulated by six families of AP2/ERF, − BHLH, , MYB, NAC, WRKY, and bZIP TFs. In addition, some MYB TFs are known to participate in the biosynthesis and metabolism of aroma volatiles in various fruits, such as FaEOBII , FaMYB98 , and FaMYB9 in strawberry fruit , and SlMYB75 in tomato fruit .…”

Section: Discussionmentioning

confidence: 99%

Transcriptome and Metabolomics Integrated Analysis Reveals MdMYB94 Associated with Esters Biosynthesis in Apple (Malus × domestica)

Yan

Tan

et al. 2023

J. Agric. Food Chem.

Volatile esters are major aromas contributing to the organoleptic quality of apple fruit. However, the molecular mechanisms underlying the regulation of volatile ester biosynthesis in apple remain elusive. This study investigated the volatile profiles and transcriptomes of 'Qinguan' (QG) apple fruit during development and/or postharvest storage. Although the constitution of volatiles varied widely between the peel and flesh, the volatile profiles of the peel and flesh of ripening QG fruit were dominated by volatile esters. WGCNA results suggested that 19 genes belonging to ester biosynthesis pathways and 11 hub transcription factor genes potentially participated in the biosynthesis and regulation of esters. To figure out key regulators of ester biosynthesis, correlation network analysis, dual-luciferase assays, and yeast one-hybrid assay were conducted and suggested that MdMYB94 transactivated the MdAAT2 promoter and participated in the regulation of ester biosynthesis. This study provides a framework for understanding ester biosynthesis and regulation in apple.