Identification, Distribution, and Pathogenicity of Diatrypaceae and Botryosphaeriaceae Associated with Citrus Branch Canker in the Southern California Desert

Mayorquin, Joey S; Wang, Danny H; Twizeyimana, M.; Eskalen, Akif

doi:10.1094/pdis-03-16-0362-re

Cited by 63 publications

(64 citation statements)

References 40 publications

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“…Indeed, agaricomycetes comprise a group of fungi that actively discharge spores primarily under humid conditions [ 5 ], including Stereum , Trametes , and Schizophyllum [ 54 – 56 ]. Peroneutypa has a closely related sordariomycete genus Eutypella [ 57 ] whose ascospores are discharged only when rainfall occurs [ 58 ]. Our observation suggests two non-mutually exclusive hypotheses: (1) spores serve as nuclei in clouds, and/or (2) spores are released selectively during precipitation.…”

Section: Discussionmentioning

confidence: 99%

Taxonomic diversity of fungi deposited from the atmosphere

Woo

et al. 2018

The ISME Journal

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Fungi release spores into the global atmosphere. The emitted spores are deposited to the surface of the Earth by sedimentation (dry deposition) and precipitation (wet deposition), and therefore contribute to the global cycling of substances. However, knowledge is scarce regarding the diversities of fungi deposited from the atmosphere. Here, an automatic dry and wet deposition sampler and high-throughput sequencing plus quantitative PCR were used to observe taxonomic diversities and flux densities of atmospheric fungal deposition. Taxon-specific fungal deposition velocities and aerodynamic diameters (da) were determined using a collocated cascade impactor for volumetric, particle-size-resolved air sampling. Large multicellular spore-producing dothideomycetes (da ≥ 10.0 μm) were predominant in dry deposition, with a mean velocity of 0.80 cm s–1 for all fungal taxa combined. Higher taxonomic richness was observed in fungal assemblages in wet deposition than in dry deposition, suggesting the presence of fungal taxa that are deposited only in wet form. In wet deposition, agaricomycetes, including mushroom-forming fungi, and sordariomycetes, including plant pathogenic species, were enriched, indicating that such fungal spores serve as nuclei in clouds, and/or are discharged preferentially during precipitation. Moreover, this study confirmed that fungal assemblage memberships and structures were significantly different between dry and wet deposition (P-test, p < 0.001). Overall, these findings suggest taxon-specific involvement of fungi in precipitation, and provide important insights into potential links between environmental changes that can disturb regional microbial communities (e.g., deforestation) and changes in precipitation patterns that might be mediated by changes in microbial communities in the atmosphere.

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Section: Discussionmentioning

confidence: 99%

Taxonomic diversity of fungi deposited from the atmosphere

Woo

et al. 2018

The ISME Journal

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“…These fungi merit adding to the list of fungal taxa causing citrus cankers worldwide ( Adesemoye et al . 2014 , Mayorquin et al . 2016 , Sandoval-Denis et al 2018 ).…”

Section: Discussionmentioning

confidence: 99%

“…2018 ), and species of Botryosphaeriaceae and Diatrypaceae ( Timmer et al . 2000 , Polizzi et al 2009 , Mayorquin et al . 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Emerging citrus diseases in Europe caused by species of Diaporthe

2017

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Species of Diaporthe are considered important plant pathogens, saprobes, and endophytes on a wide range of plant hosts. Several species are well-known on citrus, either as agents of pre- or post-harvest infections, such as dieback, melanose and stem-end rot on fruit. In this study we explored the occurrence, diversity and pathogenicity of Diaporthe species associated with Citrus and allied genera in European orchards, nurseries, and gardens. Surveys were carried out during 2015 and 2016 in Greece, Italy, Malta, Portugal, and Spain. A total of 79 Diaporthe strains were isolated from symptomatic twigs, branches and trunks. A multi-locus phylogeny was established based on five genomic loci (ITS, tef1, cal, his3 and tub2), and the morphological characters of the isolates determined. Preliminary pathogenicity tests were performed on lemon, lime, and orange plants with representative isolates. The most commonly isolated species were D. foeniculina and D. baccae, while only four isolates of D. novem were collected. Two new Diaporthe species, described here as D. limonicola and D. melitensis spp. nov. were found associated with a new devastating dieback disease of lemon plants. Furthermore, one cluster of sterile Diaporthe isolates was renamed as D. infertilis. Pathogenicity tests revealed most of the Citrus species as susceptible to D. baccae, D. foeniculina, and D. novem. Moreover, D. limonicola and D. melitensis caused serious cankers affecting all the Citrus species tested. This study is the first report of D. baccae and D. novem on citrus in Europe, and the first detection of a new Diaporthe canker disease of citrus in Europe. However, no isolates of D. citri were found. The study improves our understanding of the species associated with several disease symptoms on citrus plants, and provides useful information for effective disease management.

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“…However, more surveys are needed to clarify their host specificity. Furthermore, these species can be added to other citrus canker causing pathogens reported worldwide ( Adesemoye et al 2014 , Mayorquin et al 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Symptomatic Citrus trees reveal a new pathogenic lineage in Fusarium and two new Neocosmospora species

Sandoval-Denis¹,

Guarnaccia²,

Polizzi³

et al. 2018

persoonia

111

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The diversity of fusaria in symptomatic Citrus trees in Greece, Italy and Spain was evaluated using morphological and molecular multi-locus analyses based on fragments of the calmodulin (CAM), intergenic spacer region of the rDNA (IGS), internal transcribed spacer region of the rDNA (ITS), large subunit of the rDNA (LSU), RNA polymerase largest subunit (RPB1), RNA polymerase second largest subunit (RPB2), translation elongation factor 1-alpha (EF-1α) and beta-tubulin (TUB) genes. A total of 11 species (six Fusarium spp., and five Neocosmospora spp.) were isolated from dry root rot, crown, trunk or twig canker or twig dieback of citrus trees. The most commonly isolated species were Fusarium sarcochroum, F. oxysporum and Neocosmospora solani. Three new Fusarium species are described, i.e., F. citricola and F. salinense belonging to the newly described F. citricola species complex; and F. siculi belonging to the F. fujikuroi species complex. Results of pathogenicity tests showed this new complex to include prominent canker causing agents affecting several Citrus spp. In addition, two new species are described in Neocosmospora, named N. croci and N. macrospora, the latter species being clearly differentiated from most members of this genus by producing large, up to nine-septate sporodochial conidia.

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Identification, Distribution, and Pathogenicity of Diatrypaceae and Botryosphaeriaceae Associated with Citrus Branch Canker in the Southern California Desert

Cited by 63 publications

References 40 publications

Taxonomic diversity of fungi deposited from the atmosphere

Taxonomic diversity of fungi deposited from the atmosphere

Emerging citrus diseases in Europe caused by species of Diaporthe

Symptomatic Citrus trees reveal a new pathogenic lineage in Fusarium and two new Neocosmospora species

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