Identification, evolution and expression of a CD36 homolog in the basal chordate amphioxus Branchiostoma japonicum

Zhang, Min; Xu, Yuan; Li, Linfang; Wei, Shulei; Zhang, Shicui; Liu, Zhenhui

doi:10.1016/j.fsi.2012.11.043

Cited by 15 publications

(7 citation statements)

References 42 publications

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“…The ancestral cd36 gene in amphioxus could be up-regulated by in vivo challenge with E. coli, and by in vitro LTA stimulation of a flounder cell line transfected with an amphioxus cd36-pcDNA3 construct (Zhang et al, 2013). However, our in vivo study showed that cd36 gene expression in M. marinum-infected zebrafish embryos was down-regulated, which is in line with published data on infected adult zebrafish (van der Sar et al, 2009).…”

Section: Discussionsupporting

confidence: 91%

“…CD36 orthologs have been described in many vertebrate species such as human, mouse (Endemann et al, 1993), rat (Abumrad et al, 1993), cow (Greenwalt et al, 1990) and chicken (Shu et al, 2011). Ancestral homologs are also found in invertebrate species such as C. elegans (Means et al, 2009), sea urchin and fruit fly, and a recent study reported several homologs in amphioxus, a basal chordate (Zhang et al, 2013). Presumably due to amphioxusspecific gene duplication events there are five cd36 paralogs in this species.…”

Section: Introductionmentioning

confidence: 99%

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Molecular and functional characterization of the scavenger receptor CD36 in zebrafish and common carp

Fink

Benard

Hermsen

et al. 2015

Molecular Immunology

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CD36 is a scavenger receptor which has been studied closely in mammals where it is expressed by many different cell types and plays a role in highly diverse processes, both homeostatic and pathologic. It is among other things important in the innate immune system, in angiogenesis, and in clearance of apoptotic cells, and it is also involved in lipid metabolism and atherosclerosis. Recently, in the cephalochordate amphioxus a primitive CD36 family member was described, which was present before the divergence of CD36 from other scavenger receptor B family members, SCARB1 and SCARB2. Not much is known on the Cd36 molecule in teleost fish. We therefore studied Cd36 in both zebrafish and common carp, two closely related cyprinid fish species. Whereas a single cd36 gene is present in zebrafish, carp has two cd36 genes, and all show conserved synteny compared to mammalian CD36. The gene expression of carp cd36 is high in brain, ovary and testis but absent in immune organs. Although in mammals CD36 expression in erythrocytes, monocytes and macrophages is high, gene expression studies in leukocyte subtypes of adult carp and zebrafish larvae, including thrombocytes and macrophages provided no indication for any substantial expression of cd36 in immune cell types. Surprisingly, analysis of the cd36 promoter region does show the presence of several binding sites for transcription factors known to regulate immune responses. Overexpression of carp cd36 locates the receptor on the cell surface of mammalian cell lines consistent with the predicted topology of cyprinid Cd36 with a large extracellular domain, two transmembrane domains, and short cytoplasmic tails at both ends. Gene expression of cd36 is down-regulated during infection of zebrafish with Mycobacterium marinum, whereas knockdown of cd36 in zebrafish larvae led to higher bacterial burden upon such infection. We discuss the putative role for Cd36 in immune responses of fish in the context of other members of the scavenger receptor class B family.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Molecular and functional characterization of the scavenger receptor CD36 in zebrafish and common carp

Fink

Benard

Hermsen

et al. 2015

Molecular Immunology

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“…It has been reported that there is one CD36 gene in human, five in sea urchin and three in amphioxus [ 41 ]. Recently, in the basal chordate amphioxus Branchiostoma japonicum , a CD36 homologue was identified with functions closely involved in immune defense [ 43 ]. In this study, a CD36-like gene was down-regulated (more than 11-fold, Table 1 ) immediately after LPS challenge.…”

Section: Resultsmentioning

confidence: 99%

Expression Analysis of Immune Related Genes Identified from the Coelomocytes of Sea Cucumber (Apostichopus japonicus) in Response to LPS Challenge

Dong

Sun

Zhou

et al. 2014

IJMS

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Abstract:The sea cucumber (Apostichopus japonicus) occupies a basal position during the evolution of deuterostomes and is also an important aquaculture species. In order to identify more immune effectors, transcriptome sequencing of A. japonicus coelomocytes in response to lipopolysaccharide (LPS) challenge was performed using the Illumina HiSeq™ 2000 platform. One hundred and seven differentially expressed genes were selected and divided into four functional categories including pathogen recognition (25 genes), reorganization of cytoskeleton (27 genes), inflammation (41 genes) and apoptosis (14 genes). They were analyzed to elucidate the mechanisms of host-pathogen interactions and downstream signaling transduction. Quantitative real-time polymerase chain reactions (qRT-PCRs) of 10 representative genes validated the accuracy and reliability of RNA sequencing results with the correlation coefficients from 0.88 to 0.98 and p-value <0.05. Expression analysis of immune-related genes after LPS challenge will be useful in understanding the immune

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“…Studies on SR-like function in mammals have also indicated an important role for this family of receptors in innate immune function. SR-B1 (CD36) is widely expressed, but the expression levels vary among different tissues, being largely restricted to lymphoid and haemapoietic lineages including monocytes, macrophages, platelets, endothelium, adipocytes, smooth muscle and certain epithelia (Zhang et al, 2013). In teleosts, SR is known to be expressed by nonspecific cytotoxic cells in the peripheral blood, spleen and anterior kidney of catfish (Qiu et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Molecular characterisation and expression analysis of scavenger receptor class B member 1 (SR-B1) gene in Nile tilapia Oreochromis niloticus (Linnaeus, 1758)

Huang¹,

Cai²

2019

Indian J. Fish.

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The scavenger receptor class B member 1 (SR-B1) plays an important role in the first level of host defense against invading pathogens. In the present study, we cloned and characterized SR-B1 gene from Oreochromis niloticus (Linnaeus, 1758). The sequence of SR-B1 is 2248-bp long and contains a 1404-bp ORF encoded 467 amino acids. The sequence alignment showed that SR-B1 gene contains ten exons and nine introns. A multiple alignment analysis suggested that SR-B1 protein contains the conserved CXXS redox and GXXXG motifs, and shared high similarities with that of other species. On a phylogenetic tree, SR-B1 clustered with those of other teleosts and formed a separate fish clade. Real-time quantitative PCR analysis showed that SR-B1 was differentially expressed in various tissues and the expression levels in spleen and intestine were significantly upregulated by Streptococcus agalactiae infection. The results suggest that SR-B1 may be involved in the immune response against bacteria in Nile tilapia.

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Identification, evolution and expression of a CD36 homolog in the basal chordate amphioxus Branchiostoma japonicum

Cited by 15 publications

References 42 publications

Molecular and functional characterization of the scavenger receptor CD36 in zebrafish and common carp

Molecular and functional characterization of the scavenger receptor CD36 in zebrafish and common carp

Expression Analysis of Immune Related Genes Identified from the Coelomocytes of Sea Cucumber (Apostichopus japonicus) in Response to LPS Challenge

Molecular characterisation and expression analysis of scavenger receptor class B member 1 (SR-B1) gene in Nile tilapia Oreochromis niloticus (Linnaeus, 1758)

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