Identification for soybean host factors interacting with P3N-PIPO protein of Soybean mosaic virus

Abstract

Previous evidence suggested the potyviral frame-shift protein P3N-PIPO is required for efficient viral intercellular movement. Host proteins are essential for virus to establish successful infection, many virus proteins play key roles in the viral infection cycle by interacting with that. Here, a yeast two-hybrid screening analysis was completed using Soybean mosaic virus (SMV) P3N-PIPO as the bait and a cDNA library from soybean infected with SMV as the prey to characterize the function of SMV P3N-PIPO. Fifty-four genes were isolated and analyzed by BLAST tools. Several genes encoding proteins that interacted with SMV P3N-PIPO were screened, including genes for inhibitory and transcription factors and those related to defense, transport, and photosynthesis. Some genes encoded proteins involved in metabolic activities in the chloroplast, such as photosystem I subunit PsaD and Calvin cycle protein CP12-2. Some genes were associated with defense responses, such as pathogenesis-related protein 1-like protein, stress-related protein-like protein, and stress enhanced protein 2. Other genes encoded defense-related transcription factors, such as WRKY transcription factor 51, while others were related to signal transduction, including a translationally-controlled tumor protein homolog, calcium-transporting ATPase 12, plasma membrane-type calcium-transporting ATPase 12-like protein, and calcineurin B-like protein 1-like isoform X1. Some genes coding for proteins related to affect viral plasmodesmata tracking, such as glucan endo-1,3-b-glucosidase (acidic isoform GL153). This study is the first to preliminarily delineate the interactions between SMV P3N-PIPO and host proteins related to defense responses during SMV infection.

Genome-wide identification and analysis of long noncoding RNAs (lncRNAs) during seed development in peanut (Arachis hypogaea L.)

“…The increased expression of the UPL4 gene could be related with its role in peanut seed development. Another up-regulated lncRNA MSTRG.12404.1 targets amino acid permease 6-like (AAP6) gene, which encodes plant-specific amino acid transmembrane transporter and involved in the amino acid uptake [40]. In the present study, the expression of AAP6 gene was up-regulated at 35 DAF, indicating that AAP6 may also be involved in peanut seed development.…”

Dissecting the Molecular Mechanism of the Subcellular Localization and Cell-to-cell Movement of the Sugarcane mosaic virus P3N-PIPO

“…P3N-PIPOs have been intensively studied in several potyviruses, such as TuMV 21 , 22 , TEV 21 , TVBMV 26 and SMV 41 . The targeting of P3N-PIPOs of TuMV and TEV to the PD was confirmed by plasmolysis 21 .…”

“…Moreover, PCaP1 is not the only protein that interacts with P3N-PIPO. In total, 54 proteins, including glucan endo-1,3-β glucosidase (β-1,3-glucanase), identified from soybean ( Glycine max ) interact with SMV P3N-PIPO 41 . The β-1,3-glucanase localizes to the PD through GPI 52 , 53 .…”

Genome-wide association links candidate genes to fruit firmness, fruit flesh color, flowering time, and soluble solid content in apricot (Prunus armeniaca)

“…Putative pentatricopeptide repeat-containing protein is a member of the pentatricopeptide repeat (PPR) protein family and is involved in the organellar RNA metabolism (64). Calcium-transporting ATPase 12, plasma membrane-type-like has a function in calcium-transporting ATPase activity and calmodulin binding (65). tRNA (guanine(37)-N1)-methyltransferase 1 is responsible for the methylation of cytoplasmic and mitochondrial tRNAs in the N1 position of guanosine-37 Arabidopsis thaliana (66).…”