Identification, inheritance, and fitness costs of Cry2Ab2 resistance in a field-derived population of sugarcane borer, Diatraea saccharalis (F.) (Lepidoptera: Crambidae)

Huang, Fangneng; Chen, Mao; Gowda, A. S. Prakasha; Clark, Thomas L.; McNulty, Brain C.; Yang, Fei; Niu, Ying

doi:10.1016/j.jip.2015.07.007

Cited by 11 publications

(9 citation statements)

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“…Previously, a polygenic nature of resistance inheritance was reported for Cry2Ab in H. armigera [ 36 ], Cry1Ab, Cry1F and Cry1Ah in Ostrinia furnacalis (Guenée) [ 40 , 74 , 75 ], Cry1Ab in M. unipuncta [ 66 ], and Btk in T. ni [ 21 ]. Contrary to our results, a monogenic nature of resistance to B. thuringiensis insecticides has also been described in different pests [ 10 , 39 , 57 , 64 , 65 ]. The polygenic resistance observed in this study is advantageous because the multiple-gene-driven resistance is easier to overwhelm compared to single-gene-driven resistance.…”

Section: Discussioncontrasting

confidence: 99%

“…The current study showed that the Dipel resistance is expressed as an autosomal and incompletely dominant feature in E. vittella . An autosomal inheritance of B. thuringiensis crystal protein resistance has been reported in many insect pests [ 35 , 36 , 38 , 39 , 57 ]. The degree of dominance can fluctuate on the basis of type of B. thuringiensis crystal proteins, genetic backgrounds, individual species, ecological environments, and diverse selection histories [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

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Realized Heritability, Risk Assessment, and Inheritance Pattern in Earias vittella (Lepidoptera: Noctuidae) Resistant to Dipel (Bacillus thuringiensis Kurstaki)

Ahmad

Gulzar

Abbas

et al. 2022

Toxins

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Earias vittella Fabricius is a potential cotton and okra pest in South Asia. The realized heritability, risk assessment, and inheritance mode of Bacillus thuringiensis Kurstaki (Btk) resistance were determined in the Dipel-selected (DIPEL-SEL) E. vittella. The DIPEL-SEL strain had a 127.56-fold rise in Dipel resistance after nine generations compared to the laboratory reference strain (LAB-PK). The overlapping of 95% fiducial limits in the median lethal concentrations (LC50s) of the F1 (DIPEL-SEL♂ × LAB-PK♀) and F1ǂ (DIPEL-SEL♀ × LAB-PK♂) suggested a lack of sex linkage and an autosomal Dipel resistance. The dominance (DLC) values for the F1 (0.86) and F1ǂ (0.94) indicated incompletely dominant resistance to Dipel. Backcrossing of the F1♀ × Lab-PK♂ revealed a polygenic response of resistance to Dipel. The realized heritability estimation (h2) of resistance to Dipel was 0.19. With 20% to 90% selection mortality, the generations required for a tenfold increase in LC50 of Dipel were 4.7–22.8, 3.1–14.9, and 2.3–11.1 at h2 of 0.19, 0.29, and 0.39, respectively, and a constant slope of 1.56. At slope values of 2.56 and 3.56 with a constant h2 = 0.19, 7.7–37.4 and 10.6–52.0 generations were needed to increase the tenfold LC50 of Dipel in the DIPEL-SEL E. vittella. It is concluded that the DIPEL-SEL E. vittella has an autosomal, incompletely dominant, and polygenic nature of resistance. The h2 of 0.19 suggested that a high proportion of phenotypic variation for the Dipel resistance in E. vittella was heritable genetic variation. The present results will support the creation of an effective and suitable resistance management plan for better control of E. vittella.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Realized Heritability, Risk Assessment, and Inheritance Pattern in Earias vittella (Lepidoptera: Noctuidae) Resistant to Dipel (Bacillus thuringiensis Kurstaki)

Ahmad

Gulzar

Abbas

et al. 2022

Toxins

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“…In addition, dominance levels of a Cry2Ab2-resistant population of D. saccharalis were also evaluated based on 7-d survivorship rates on leaf tissue of a Cry2Ab2 maize experimental line. 88 ,The Cry2Ab2-resostant strain was documented to process a major resistance allele to the experimental Cry2Ab2 maize line. However, the experimental line used in the study expressed a relatively low level of Cry2Ab2 protein (FH personal communication), and thus, the case of the Cry2Ab2 resistance in D. saccharalis was excluded in this review.…”

Section: Disclosure Of Potential Conflict Of Interestmentioning

confidence: 99%

Dominance and fitness costs of insect resistance to genetically modified Bacillus thuringiensis crops

Huang

2020

GM Crops & Food

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Evolution of resistance to genetically modified Bacillus thuringiensis (Bt) crops in pest populations is a major threat to the sustainability of the technology. Incidents of field resistance that have led to control problems of Bt crops or significantly reduced susceptibility of individual Bt proteins in pyramided plants have increased dramatically across the world, especially in recent years. Analysis of globally published data showed that 61.5% and 60.0% of the cases of resistance with major alleles that allowed homozygous resistant genotypes to survival on Bt crops were functionally non-recessive and did not involve fitness costs, respectively. Dominance levels (D FL s) measured on Bt plants ranged from −0.02 to 1.56 with a mean (± sem) of 0.35 ± 0.13 for the 13 cases of single-gene resistance to Bt plants that have been evaluated. Among these, all six cases with field control problems were functionally non-recessive with a mean D FL of 0.63 ± 0.24, which was significantly greater than the D FL (0.11 ± 0.07) of the seven cases without field resistance. In addition, index of fitness costs (IFC) of major resistance was calculated for each case based on the fitness of resistant (R’R’) and heterozygous (R’S’) genotypes on non-Bt plants divided by the fitness of their susceptible (S’S’) counterparts. The estimated IFCs for 15 cases of single-gene resistance were similar for R’R’ and R’S’, and for the cases with and without field resistance; and the values averaged 1.10 ± 0.12 for R’R’ and 1.20 ± 0.18 for R’S’. Limited published data suggest that resistance of insects to dual/multiple-gene Bt crops is likely to be more recessive than the related single-gene resistance, but their IFCs are similar. The quantitative analysis of the global data documents that the prevalence of non-recessive resistance has played an essential role in the widespread evolution of resistance to Bt crops, while the lack of fitness costs is apparently not as critical as the non-recessive resistance. The results suggest that planting of ‘high dose’ traits is an effective method for Bt crop IRM and more comprehensive management strategies that are also effective for functionally non-recessive resistance should be deployed.

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“…In the United States, the F 2 screen has been conducted to investigate RAFs to Cry1Ab and Cry2Ab2 in D. saccharalis populations from maize fields. 26,27 In addition, field resistance of D. saccharalis to Cry1F and Cry1A.105 in maize has been reported in Argentina. 10 In Brazil, Girón-Pérez et al reported varied Cry1Ab susceptibilities among several laboratory colonies of D. saccharalis; and larvae of the laboratory colonies responded positively to selections with Cry1Ab maize leaf tissue.…”

Section: Introductionmentioning

confidence: 99%

Varied frequencies of resistance alleles to Cry1Ab and Cry1Ac among Brazilian populations of the sugarcane borer, Diatraea saccharalis (F.)

Oliveira

Sakuno

Miraldo

et al. 2022

Pest Management Science

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BACKGROUND: Brazil is the largest grower of the world's 26 million ha of sugarcane, Saccharum officinarum. Pest damage mainly by the sugarcane borer, Diatraea saccharalis (F.), is a great challenge to the sugarcane industry. To control D. saccharalis, Brazil launched the world's first commercial use of Bt sugarcane in 2017. As part of the resistance management programs for Bt sugarcane planting, 535 F 2 isoline families of D. saccharalis collected from three major sugarcane planting states (Goiás, Minas Gerais and São Paulo) in Brazil during 2019-2020 were screened for resistance to two Bt sugarcane varieties: CTC20BT expressing Cry1Ab and CTC9001BT expressing Cry1Ac. Here we report the results of the first study related to Bt resistance in a sugarcane cropping system. RESULTS: Larval survivorships of these families in an F 2 screen on CTC20BT were highly correlated with their survival on CTC9001BT, whereas the Cry1Ac tissues exhibited greater insecticidal activities than Cry1Ab. Resistance allele frequencies (RAFs) for populations from Goiás and Minas Gerais were relatively low at 0.0034 for Cry1Ab and 0.0045 to Cry1Ac. By contrast, RAFs for São Paulo populations were considerably greater (0.0393 to Cry1Ab, 0.0245 to Cry1Ac).CONCLUSIONS: RAFs to Cry1Ab and Cry1Ac varied among Brazilian D. saccharalis populations. Prior selection resulting from an intensive use of single-gene Bt maize under low compliance of refuge planting could be a main factor contributing to the high RAF in São Paulo. The results suggest that mitigation measures including sufficient non-Bt maize refuge planting, effective resistance monitoring, and use of pyramided Bt sugarcane traits should be implemented promptly to prevent further increase in the RAF to ensure the sustainable use of Bt sugarcane in Brazil.

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Identification, inheritance, and fitness costs of Cry2Ab2 resistance in a field-derived population of sugarcane borer, Diatraea saccharalis (F.) (Lepidoptera: Crambidae)

Cited by 11 publications

References 44 publications

Realized Heritability, Risk Assessment, and Inheritance Pattern in Earias vittella (Lepidoptera: Noctuidae) Resistant to Dipel (Bacillus thuringiensis Kurstaki)

Realized Heritability, Risk Assessment, and Inheritance Pattern in Earias vittella (Lepidoptera: Noctuidae) Resistant to Dipel (Bacillus thuringiensis Kurstaki)

Dominance and fitness costs of insect resistance to genetically modified Bacillus thuringiensis crops

Varied frequencies of resistance alleles to Cry1Ab and Cry1Ac among Brazilian populations of the sugarcane borer, Diatraea saccharalis (F.)

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