Identification, Inheritance, and Variation of Microsatellite Markers in the Black Scallop Mimachlamys varia

Abstract

Five polymorphic microsatellite loci were identified in the black scallop Mimachlamys varia after construction of a genomic library enriched for (GT)n. To examine the transmission pattern of microsatellite alleles, several families were created and genotypes scored for three loci. The expected Mendelian ratios were found in 12 of 14 segregations examined. Unexpected segregations may be explained by a genotyping error (allelic dropout), given that when a specific allele was treated as dominant, the phenotypic ratios conformed to Mendelian expectations. The five loci were also examined in two samples from the Spanish coast. The two localities displayed similar mean values for the number of alleles per locus (7.2-8.4), allelic richness (7.2-7.9), and observed (0.389-0.484) and expected heterozygosity (0.545-0.618). Significant Hardy-Weinberg deviations were observed at three loci, with heterozygote deficiency occurring in all cases. Global multilocus θ value and allele frequencies at one locus revealed significant differentiation between the two localities.

Investigation of possible epistatic interactions between GRIA2 and GRIA4 variants on clinical outcomes in patients with major depressive disorder

“…13 There is, however, some evidence to suggest that epistatic interactions can be observed, even when single SNPs considered on an individual basis do not lead to positive findings. 20,21 Such a possibility raises substantial computational and theoretical difficulties, when considering all possible gene-gene interactions and other forms of gene-expression control. 22 No statistically significant effects were observed regarding any of the secondary outcome measures in the presence of both GRIA2 rs4260586 and GRIA4 rs10736648 polymorphisms, further suggesting the possibility that no relevant epistatic interactions exist between the two SNPs under investigation.…”

“…13 There is, however, some evidence to suggest that epistatic interactions can be observed, even when single SNPs considered on an individual basis do not lead to positive findings. 20,21 Such a possibility raises substantial computational and theoretical difficulties, when considering all possible gene–gene interactions and other forms of gene-expression control. 22…”