Identification, Isolation, Cloning, and Expression of a New Alkaline Serine Protease Gene from Native Iranian Bacillus sp. RAM 53 for Use in the Industry

Alei, Aliakbar; Moradian, Fatemeh; Farhadi, Ayoub; Rostami, Mehdi

doi:10.1007/s12010-023-04585-9

Appl Biochem Biotechnol

2023

DOI: 10.1007/s12010-023-04585-9

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Identification, Isolation, Cloning, and Expression of a New Alkaline Serine Protease Gene from Native Iranian Bacillus sp. RAM 53 for Use in the Industry

Aliakbar Alei,

Fatemeh Moradian,

Ayoub Farhadi

et al.

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2024

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Cited by 4 publications

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Cloning, purification, and functional characterization of recombinant pullulanase from Bacillus cereusATCC 14579 for improved detergent performance

Zafar,

Masood,

Rahman

et al. 2024

J Surfact & Detergents

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The present study outlines the approach that was employed for cloning, expression, and characterization of the recombinant pullulanase enzyme from Bacillus cereus ATCC 14579 into Escherichia coli BL21(DE3) using pET‐25b (+) expression vector. The recombinant pullulanase enzyme was purified using ammonium sulfate precipitation and immobilized metal ion affinity chromatography (IMAC). The molecular mass of the purified pullulanase enzyme was measured using sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) as 95 kDa. The purified recombinant pullulanase enzyme demonstrated significant thermal stability, maintaining its structural integrity and functionality at temperatures as high as 90°C over a period of 4 h. The inclusion of divalent metal ions, specifically Ca2+ and Mg2+, had a positive effect on the activity of the pullulanase enzyme. Conversely, the presence of Co2+ and EDTA (Ethylene Diamine Tetra Acetic acid) resulted in suppression of the enzyme activity. The purified pullulanase enzyme demonstrated remarkable resistance when exposed to organic solvents. The enzyme activity was notably decreased in the presence of SDS (Sodium Dodecyl Sulfate) while β‐mercaptoethanol and tween‐60 did not substantially affect the enzyme activity and stability which suggest its potential applicability in the detergent sector. This discovery indicates a potential approach to improve the effectiveness of currently available detergents in the marketplace.

show abstract

Cloning, purification, and functional characterization of recombinant pullulanase from Bacillus cereusATCC 14579 for improved detergent performance

Zafar,

Masood,

Rahman

et al. 2024

J Surfact & Detergents

View full text Add to dashboard Cite

show abstract

3D vat photopolymerization printing of hydrophilic silicone-based microfluidic devices and the effect of cellulose nanocrystals as additives for improved printing accuracy

Wong,

Ganguly,

Tang

2024

Additive Manufacturing

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Molecular analysis of recombinant collagenase from Bacillus siamensis strain Z1: Gene Cloning, expression and in-silico characterization

Revankar,

Bagewadi,

Ahmed Shaikh

et al. 2024

Arabian Journal of Chemistry

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Identification, Isolation, Cloning, and Expression of a New Alkaline Serine Protease Gene from Native Iranian Bacillus sp. RAM 53 for Use in the Industry

Cited by 4 publications

References 44 publications

Cloning, purification, and functional characterization of recombinant pullulanase from Bacillus cereusATCC 14579 for improved detergent performance

Cloning, purification, and functional characterization of recombinant pullulanase from Bacillus cereusATCC 14579 for improved detergent performance

3D vat photopolymerization printing of hydrophilic silicone-based microfluidic devices and the effect of cellulose nanocrystals as additives for improved printing accuracy

Molecular analysis of recombinant collagenase from Bacillus siamensis strain Z1: Gene Cloning, expression and in-silico characterization

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