Identification, localization and possible role of calmodulin like protein in phospholipid synthesis of Microsporum gypseum

Bindra, Anu; Giri, Shailendra; Khuller, G. K.

doi:10.1016/0005-2760(94)00226-o

Cited by 7 publications

(6 citation statements)

References 13 publications

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“…At this concentration, a significant reduction (40%) in cell mass was seen and there was 27 and 15% reduction in 14 C‐acetate incorporation in total lipids and phospholipids respectively, in W‐7 grown cells as compared to the controls (Table 3). These observations are in agreement with previous findings of uptake of labelled acetate in the presence of CaM antagonist in M. gypseum (Bindra et al. 1995), Cyanobacteria (Kerson et al.…”

Section: Resultssupporting

confidence: 94%

“…At 40 lM M concentration of W-7, there was maximum inhibition of acetate incorporation into total lipids and phospholipids (Table 1). This is in agreement with the inhibition seen in the incorporation of labelled precursor into phospholipids of Microsporum gypseum (Bindra et al 1995) and Mycobacterium smegmatis (Bura et al 1991) when treated with CaM antagonists. Single dimensional thin layer chromatography of total phospholipids extracted from C. albicans after incubation with 40 lM M W-7 was carried out to see the effect of W-7 on individual phospholipids.…”

Section: Resultssupporting

confidence: 89%

“…At 40 μ m concentration of W‐7, there was maximum inhibition of acetate incorporation into total lipids and phospholipids (Table 1). This is in agreement with the inhibition seen in the incorporation of labelled precursor into phospholipids of Microsporum gypseum (Bindra et al. 1995) and Mycobacterium smegmatis (Bura et al.…”

Section: Resultssupporting

confidence: 89%

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Influence of W-7, a calmodulin antagonist on phospholipid biosynthesis inCandida albicans

Dhillon

Sharma

Khuller

2003

Letters in Applied Microbiology

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Aim: This study was undertaken to investigate the role of calmodulin in phospholipid biosynthesis in Candida albicans using W-7, a calmodulin antagonist. Methods: Cells were grown as shake cultures in the absence and presence of W-7 at different concentrations. Changes in cell mass, phospholipid content and incorporation of labelled precursor into phospholipid and activities of respective enzymes have been studied. Results: Decreased incorporation of labelled acetate into total lipids and phospholipids was observed in the presence of 40 lM M of W-7 which was not as a consequence of altered growth of Candida in the presence of calmodulin antagonist. Further, a significant decrease in the levels of calmodulin and CaM dependent protein kinase activity was observed in cells grown with different concentrations of W-7. This was accompanied by decreased/ increased activity of phosphatidic acid phosphatase and phospholipase A, respectively in W-7 grown cells as compared to controls. Conclusions: These findings suggest definite involvement of calmodulin in the regulation of phospholipid metabolism in Candida albicans.

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Section: Resultssupporting

confidence: 94%

Section: Resultssupporting

confidence: 89%

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Influence of W-7, a calmodulin antagonist on phospholipid biosynthesis inCandida albicans

Dhillon

Sharma

Khuller

2003

Letters in Applied Microbiology

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“…In Microsporum gypseum, a dermatophyte, calmodulin-like protein hasbeen identified and purified but no specific function was assigned to it (50). The effect of calcium on phospholipid synthesis is possibly mediated by calmodulin as suggested in earlier studies (48).…”

Section: Regulation Of Phospholipids By Calc~uml Calmodulinmentioning

confidence: 98%

Dermatophyte lipids—Composition and regulation of phospholipids

Khuller

Sharma

Deo

2000

Indian J Clin Biochem

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This review presents the extensive work carried out on lipid components of dermatophytes, their biosynthesis, turnover and regulation. It emerges from the work done so far that the pathways of lipid biosynthesis/degradation and the lipid composition in dermatophytes are similar to those in yeasts and other fungi. Second messengers (Ca 2 § cAMP) were demonstrated to have a regulatory role in phospholipid metabolism and they mainly act by stimulating Ca2*/CaM or cAMP dependent protein kinase(s). Both these kinases were purified and characterized in Microsporum gypseum. Further work is being carried out to elucidate the molecular mechanism of regulation of phospholipid metabolism by these second messengers.

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“…It is interesting to note that a fraction of defective zoospores were released by the cultures that received late additions of chlorpromazine or EGTA, which suggests that calcium-and calmodulin-dependent events also happen during cleavage of cytoplasm and formation of zoospores when there is an intensive lipid synthesis [10]. Accordingly, it was demonstrated that phospholipid biosynthesis in Microsporum gypseum is dependent on calcium and calmodulin functions [22]. The increase of intracellular calcium, mediated by growth factors and/or inositol triphosphate, is essential to functions related to the cellular cycle, such as activation of mitogenic and transcription factors [23].…”

Section: Discussionmentioning

confidence: 99%

The induction of sporulation in the aquatic fungus Blastocladiella emersonii is dependent on extracellular calcium

Coutinho¹

1999

FEMS Microbiology Letters

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Induction of sporulation in Blastocladiella emersonii is absolutely dependent on extracellular calcium. Vegetative cells grown in media with or without calcium do not sporulate in media devoid of calcium or in CaCl 2 with EGTA. Calcium channel blockers, CoCl 2 and nifedipine, and ionophore A23187 inhibited the induction of sporulation. The calmodulin antagonists trifluoperazine and chlorpromazine inhibited the sporulation when present in the cultures at least 60 min after induction. So, calcium that is accumulated during growth is not sufficient or is not mobilized to initiate sporulation, and a calcium influx is likely to occur by type II calcium channel functions, essential for the response to nutritional starvation. A calmodulin-like protein has been suggested to mediate calcium events in sporulation. ß

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Identification, localization and possible role of calmodulin like protein in phospholipid synthesis of Microsporum gypseum

Cited by 7 publications

References 13 publications

Influence of W-7, a calmodulin antagonist on phospholipid biosynthesis inCandida albicans

Influence of W-7, a calmodulin antagonist on phospholipid biosynthesis inCandida albicans

Dermatophyte lipids—Composition and regulation of phospholipids

The induction of sporulation in the aquatic fungus Blastocladiella emersonii is dependent on extracellular calcium

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