Identification, Molecular Cloning, and Functional Characterization of a Wheat UDP-Glucosyltransferase Involved in Resistance to Fusarium Head Blight and to Mycotoxin Accumulation

Abstract

Plant uridine diphosphate (UDP)-glucosyltransferases (UGT) catalyze the glucosylation of xenobiotic, endogenous substrates and phytotoxic agents produced by pathogens such as mycotoxins. The Bradi5g03300 UGT-encoding gene from the model plant Brachypodium distachyon was previously shown to confer tolerance to the mycotoxin deoxynivalenol (DON) through glucosylation into DON 3-O-glucose (D3G). This gene was shown to be involved in early establishment of quantitative resistance to Fusarium Head Blight, a major disease of small-grain cereals. In the present work, using a translational biology approach, we identified and characterized a wheat candidate gene, Traes_2BS_14CA35D5D, orthologous to Bradi5g03300 on the short arm of chromosome 2B of bread wheat (Triticum aestivum L.). We showed that this UGT-encoding gene was highly inducible upon infection by a DON-producing Fusarium graminearum strain while not induced upon infection by a strain unable to produce DON. Transformation of this wheat UGT-encoding gene into B. distachyon revealed its ability to confer FHB resistance and root tolerance to DON as well as to potentially conjugate DON into D3G in planta and its impact on total DON reduction. In conclusion, we provide a UGT-encoding candidate gene to include in selection process for FHB resistance.

TaUGT6, a Novel UDP-Glycosyltransferase Gene Enhances the Resistance to FHB and DON Accumulation in Wheat

“…graminearum inoculation, but no further functional analysis was conducted ( Lin et al, 2008 ; Ma et al, 2015 ). Thus far, only TaUGT3 , TaUGT5 , and Traes_2BS_14CA35D5D (orthologous to Bradi5g03300 ) in wheat have been transformed into plants to prove their role in FHB resistance and DON tolerance ( Gatti et al, 2018 ; Xing et al, 2018 ; Zhao et al, 2018 ). Thus, discovering more UGT genes involved in FHB resistance will help elucidate the host resistance mechanisms that confer resistance to DON and enhance DON detoxification mechanisms and will promote the development of more strategies for improving FHB resistance.…”

“…The expression of Traes_2BS_14CA35D5D , orthologous to Bradi5g03300 , is highly inducible by DON, and transformation of this UGT -encoding gene from wheat into B . distachyon enhanced FHB resistance and root tolerance to DON ( Gatti et al, 2018 ). In this study, we cloned and characterized a novel UGT gene TaUGT6 involved in resistance to DON tolerance and Fusarium spread.…”

Validation and delineation of a locus conferring Fusarium crown rot resistance on 1HL in barley by analysing transcriptomes from multiple pairs of near isogenic lines

“…The Fusarium mycotoxin, deoxynivalenol has been shown to be required for full virulence of F. pseudograminearum when infecting wheat and Brachypodium [49, 50]. Detoxification of deoxynivalenol has been strongly implicated in defence against F. graminearum causing Fusarium head blight with DON detoxifying UDP glycosyltransferases identified in wheat, barley and Brachypodium [51–53]. The UDP-glycosyltransferase detoxifying DON in barley ( HORVU5Hr1G047150 ) [52, 54] was not found to be differentially expressed between or showing SNPs differences between R or S isolines in the current study (Additional file 5: Table S5).…”

Transcriptomic and Proteomic Analyses of a New Cytoplasmic Male Sterile Line with wildGossypium bickii.Genetic Background

“…), thus regulating the location of the receptor molecule in the cell and its biological activities such as solubility and transport in organisms (35, 36) . UGT plays an important role in regulating glycosylation and energy storage of secondary metabolites in organisms, endogenous hormone activity and toxicity relief of exogenous toxins (37, 38) . In this study, two different primers (E2M7 and E15M3) were used to amplify the difference of the highest consistency with uridine diphosphate glucosyltransferase gene in the buds of the YA-CMS plant during the peak period of abortion (sporogenous cell proliferation stage, microspore mother cell stage and meiosis stage of microspore development).…”