Identification of 1-aminocyclopropane-1-carboxylic acid synthase genes controlling the ethylene level of ripening fruit in Japanese pear (Pyrus pyrifolia Nakai)

Itai, Akihiro; Kawata, Tomio; Tanabe, Kenji; Tamura, Fumio; Uchiyama, Masaharu; Tomomitsu, Masao; Shiraiwa, Nobutaka

doi:10.1007/s004380050939

Cited by 81 publications

(79 citation statements)

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“…h −1 during ripening (Itai et al 2002). Differences in the ethylene production were thought to be regulated mainly by differential expression of ethylene biosynthetic genes of ACCsynthase (Itai et al 1999;2002; Ripening behaviour of some attached and detached fruits Many fruits that belong to climacteric group such as apple and pear enter the climacteric phase soon after harvest but they might not ripe fully for weeks if left on the tree (Gerhardt 1947). The best example for such a differential behaviour is the avocado fruit.…”

Section: Fruit Ripeningmentioning

confidence: 99%

The fading distinctions between classical patterns of ripening in climacteric and non-climacteric fruit and the ubiquity of ethylene—An overview

2011

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The process of fruit ripening is normally viewed distinctly in climacteric and non-climacteric fruits. But, many fruits such as guava, melon, Japanese plum, Asian pear and pepper show climacteric as well as non-climacteric behaviour depending on the cultivar or genotype. Investigations on in planta levels of CO 2 and ethylene at various stages of fruits during ripening supported the role and involvement of changes in the rate of respiration and ethylene production in non-climacteric fruits such as strawberry, grapes and citrus. Non-climacteric fruits are also reported to respond to the exogenous application of ethylene. Comparative analysis of plant-attached and plant-detached fruits did not show similarity in their ripening behaviour. This disparity is being explained in view of 1. Hypothetical ripening inhibitor, 2. Differences in the production, release and endogenous levels of ethylene, 3. Sensitivity of fruits towards ethylene and 4. Variations in the gaseous microenvironment among fruits and their varieties. Detailed studies on genetic and inheritance patterns along with the application of '-omics' research indicated that ethylene-dependent and ethylene-independent pathways coexist in both climacteric and non-climacteric fruits. Auxin levels also interact with ethylene in regulating ripening. These findings therefore reveal that the classification of fruits based on climacteric rise and/or ethylene production status is not very distinct or perfect. However, presence of a characteristic rise in CO 2 levels and a burst in ethylene production in some non-climacteric fruits as well as the presence of system 2 of ethylene production point to a ubiquitous role for ethylene in fruit ripening.

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Section: Fruit Ripeningmentioning

confidence: 99%

The fading distinctions between classical patterns of ripening in climacteric and non-climacteric fruit and the ubiquity of ethylene—An overview

2011

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“…Through PpACS1 protein, there are only one N-glycosylation site (PGSs; N in NxS/T motifs, positions 422) and cAMP-and cGMP-dependent protein kinase phosphorylation site (R 307 RMS 310 ). Sequence comparison revealed that PpACS1 shares the highest similarity (97% identity) with PcACS2b (El-Sharkawy et al, 2004) and pPPACS2 (Itai et al, 1999). It shares relatively high homology (96% identity) with MdACS3a-1 and MdACS3a-2, but relatively low homology (83-95% identities) with pPPACS1 (Itai et al, 1999) and other known plant ACSs (Fig.1).…”

Section: Structural Analysis Of the Ppacs1 Proteinmentioning

confidence: 97%

“…Sequence comparison revealed that PpACS1 shares the highest similarity (97% identity) with PcACS2b (El-Sharkawy et al, 2004) and pPPACS2 (Itai et al, 1999). It shares relatively high homology (96% identity) with MdACS3a-1 and MdACS3a-2, but relatively low homology (83-95% identities) with pPPACS1 (Itai et al, 1999) and other known plant ACSs (Fig.1). This conserved structure may be related to the maintenance of ACS functions for plant development.…”

Section: Structural Analysis Of the Ppacs1 Proteinmentioning

confidence: 97%

“…As shown in Fig.3, these proteins obviously split into two subgroups. PpACS1, PcACS2b (El-Sharkawy et al, 2004), PcACS2a (El-Sharkawy et al, 2004, pPPACS2 (Itai et al, 1999), MdACS3a-2, MdACS3a-1, MdACS3c (Wang et al, 2009), PcACS3a, MdACS3b (Wang et al, 2009) and AtACS7 (Mayer et al, 1999) form one subgroup, while PbACS1B, pPPACS1 (Itai et al, 1999) and PcACS1b (El-Sharkawy et al, 2004) together with the Arabidopsis ACS8 are located in the second branch of the tree. These results suggest that the divergence could have occurred before the differentiation of the plants.…”

Section: Phylogenetic Relationship Of Ppacs Proteinmentioning

confidence: 99%

“…Lots of important ACC synthase genes have been isolated and characterized from the plant kingdom. For examples, ACS genes were identified in pear (Pyrus communis) (El-Sharkawy et al, 2004), Japanese pear (Pyrus pyrifolia) (Itai et al, 1999), apple (Rosenfield et al, 1996;Wang et al, 2009), peach (Mathooko et al, 2001;Tatsuki et al, 2006), pineapple (Cazzonelli et al, 1998;Botella et al, 2000;Trusov et al, 2006), plum (El-Sharkawy et al, 2008), cucumber (Mathooko et al, 1999), watermelon (Salman-Minkov et al, 2008), Arabidopsis (Rodrigues-Pousada et al, 1993;Mayer et al, 1999) , tomato (Nakatsuka et al, 1997), etc.…”

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confidence: 99%

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Molecular Characterization of Pear 1-Aminocyclopropane-1-Carboxylate Synthase Gene Preferentially Expressed in Leaves

Shi¹,

Zhang²,

Sun³

et al. 2012

JAS

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Abstract1-Aminocyclopropane-1-carboxylate (ACC) synthase catalyzes the conversion of S-adenosyl-L-methionine to ACC in the ethylene biosynthetic pathway. Lots of important ACC synthase genes have been isolated and characterized from the plant kingdom. In this study, a cDNA clone encoding putative ACC synthase was isolated from a cDNA library produced using mRNA from pear (Pyrus pyrifolia). The cDNA clone, designated PpACS1 (GenBank accession No. JQ284383), comprised an open reading frame of 1, 341 bp encoding a protein of 446 amino acids that shares high similarity with the known plant ACSs. Using PCR amplification techniques, a genomic clone corresponding to PpACS1 was isolated and shown to contain two introns with typical GT/AG boundaries defining the splice junctions. The PpACS1 gene product shared 97% identity with an ACC synthase from pear (Pyrus communis), and phylogenetic analyses clearly placed the gene product in the ACC synthase cluster of the plant ACS superfamily tree. RT-PCR analysis indicated that the PpACS1 gene was preferentially expressed in pear leaves. The transcript of PpACS1 gene was accumulated at relatively high levels in anthers. The expression signal was detected in shoot at relatively low levels, but none signal was detected in developing fruit of pear. These results suggested that the PpACS1 may participate in the regulation of ethylene production in pear leaves and anthers.

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2003

Fruit and Vegetables

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Identification of 1-aminocyclopropane-1-carboxylic acid synthase genes controlling the ethylene level of ripening fruit in Japanese pear (Pyrus pyrifolia Nakai)

Cited by 81 publications

References 28 publications

The fading distinctions between classical patterns of ripening in climacteric and non-climacteric fruit and the ubiquity of ethylene—An overview

The fading distinctions between classical patterns of ripening in climacteric and non-climacteric fruit and the ubiquity of ethylene—An overview

Molecular Characterization of Pear 1-Aminocyclopropane-1-Carboxylate Synthase Gene Preferentially Expressed in Leaves

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