Identification of 12 novel RHD alleles in western France by denaturing high‐performance liquid chromatography analysis

Abstract

The identification of 12 novel RHD alleles represents a significant addition to the known repertoire of unusual RHD variants and, at the same time, serves to deepen our understanding of the molecular basis of weak and partial D. The accurate molecular typing of RHD alleles would allow to reduce the alloimmunization risk.

It's time to phase inRHDgenotyping for patients with a serologic weakDphenotype

“…31 Alloimmunization has been observed with certain other weak D types, including weak D type 4.2, 33,55 (DAR), 31 type 11, 33,55 type 15, 33,55 type 21, 71 and type 57. 60 The Work Group reviewed published and unpublished reports to determine whether weak D types 4.0 and 4.1 should be recommended as weak D types that can be managed safely as RhD-positive. There are no published reports of allo-or autoanti-D in persons with weak D type 4.1 in large European cohorts 55,75 despite their prevalence 33,56 and frequent transfusions with RhD-positive RBCs.…”

Variant screening of the RHD gene in a large cohort of subjects with D phenotype ambiguity: report of 17 novel rare alleles

“…Variant screening of the RHD gene was performed as previously described. 10 Briefly, hemizygosity of the most common RHD deletion was first assessed by a long-range polymerase chain reaction (PCR). Hemizygous DNA samples (n = 924) were then screened for hybrid D-CE genes resulting from gene conversion events in the other allele, with RHD-specific PCR primers.…”

Diverse and novel RHD variants in Australian blood donors with a weak D phenotype: implication for transfusion management

“…The distribution of RHD variants associated with the weak D phenotype is population dependent. Weak D types 1, 2 and 3 are most frequent in European and Canadian populations [6,8,10,[18][19][20]. Weak D types 4Á0 and 4Á1 have been reported as frequently occurring alleles in African populations from Egypt, Tunisia, Ethiopia and South Africa [7,21,22].…”