Identification of 14-3-3-like protein in sugarcane (Saccharum officinarum)

Kuramae, Eiko E.; Fenille, Roseli Chela; Rosa, Vicente E. De

doi:10.1590/s1415-47572001000100007

Cited by 4 publications

(4 citation statements)

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“…Another study indicates that sugarcane contains 14-3-3 like proteins. These proteins are similar to protein 14-3-3s that found in neurons' cytoplasm and mammals' brain synapse membrane (23) . In cytoplasm, protein 14-3-3s binds with Bax.…”

Section: Discussionsupporting

confidence: 66%

Ethanol Extract of Black Sugarcane Decrease Ischemia Volume and Bax Expression In Rats’ Brain

Handayani

Nur

Kuswati

et al. 2020

Int. J. Hum. Health Sci.

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Background:Black sugarcane contains policosanol, quercetin antioxidant and 14-3-3 protein. These compounds are proven in a pre-clinical test of their ability to reduce brain ischemia. There are no pre-clinic test that prove the neuro-protectant act of black sugarcane extract on ischemia-induced rats, and how its mechanism to protect brain is not yet clear. This study’s purpose is to find the effect of ethanol extract of black sugarcane (BSEE) towards brains’ ischemic volume and to find is there any Bax role on the BSEE’s neuroprotectant mechanism.Methods: This study used fifteen male rats (Wistar). The rats were divided into three groups: group A that had been induced with BCCAO and received BSSE (group A), the rats that had been induced with BCCAO (group B), and sham group (group C). BSEE were given for a week, before bilateral ligation of common carotid artery. The BCCAO duration is 20 minutes, reperfusion periods of 2 hours. Rats’ head decapitation is done two hours after ligation. Brain tissues were colored with TTC. Ischemic brain area were analyzed using Image I software. Bax’ expression scoring was done in the brain preparations with immonohistochemical with Anti-Bax antibody. Anti-Bax proteins were anylized using ALLRED Score, while the statistic analyzing was done with One Way ANOVA.Results: There is a difference of ischemia volume’s mean (p=0,018) and bax protein expression (p=0,013) on the rats brain.Conclusion: BSEE is able to reduce ischemia volume and Bax protein expression on the rats’ brain.International Journal of Human and Health Sciences Vol. 04 No. 02 April’20 Page : 102-108

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Section: Discussionsupporting

confidence: 66%

Ethanol Extract of Black Sugarcane Decrease Ischemia Volume and Bax Expression In Rats’ Brain

Handayani

Nur

Kuswati

et al. 2020

Int. J. Hum. Health Sci.

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“…O aumento no acúmulo de transcritos codificando a proteína 14-3-3 em raízes colonizadas por G. clarum foi de aproximadamente 9 vezes em relação a raízes colonizadas cultivadas em condições de baixo P e de aproximadamente 10 vezes em relação a raízes não-inoculadas cultivadas em alto P. As proteínas 14-3-3 estão associadas a regulação da expressão gênica em células vegetais, devido à formação de complexos que ligamse a "boxes G" (Kuramae et al, 2001). A superexpressão do fator de transcrição AP2 em plantas de tabaco transformadas aumentou a tolerância destas plantas ao ataque de patógenos e ao estresse osmótico (Park et al, 2001).…”

Section: Análiseunclassified

Análise de seqüências expressas em raízes de cana-de-açúcar colonizadas por Glomus clarum

Takahashi¹

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“…The 14-3-3 proteins are from a family of regulatory molecules, with 9 to 10 conserved domains of the α-helix type (Lapointe et al, 2001) that modulate various cellular processes such as cell cycle, metabolism, stress response and signal transduction (Aitken, 2006;Shin et al, 2011). The association of those proteins with the G-box binding factor suggests that they are directly involved in transcriptional regulation (Kuramae;Fenille;Jr 2001). In sugarcane, these proteins were expressed in all of the cDNA libraries obtained from different tissue (meristem, callus, flower, sprout, leaves, root, stalk, seed) and of seedlings infected with bacteria (Kuramae;Fenille;Jr, 2001).…”

Section: Gimentioning

confidence: 99%

“…The association of those proteins with the G-box binding factor suggests that they are directly involved in transcriptional regulation (Kuramae;Fenille;Jr 2001). In sugarcane, these proteins were expressed in all of the cDNA libraries obtained from different tissue (meristem, callus, flower, sprout, leaves, root, stalk, seed) and of seedlings infected with bacteria (Kuramae;Fenille;Jr, 2001). However, in this experiment, healthy and sick plants presented similar levels of expression for this gene (Figure1), so maybe an eventual flaw occurred in the subtraction process leading to the amplification of segments that are not differentially expressed.…”

Section: Gimentioning

confidence: 99%

IDENTIFICATION AND QUANTIFICATION OF DIFFERENTIALLY EXPRESSED GENES ASSOCIATED WITH CITRUS BLIGHT (Citrus spp.)

et al. 2015

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Brazil is the largest citrus producer in the world, being responsible for more than 20% of its production, which is, however still low due to phytosanitary issues such as citrus blight. Citrus blight is an anomaly whose causes still have not yet been determined, therefore there are no efficient control measures to minimize the production losses with the use of resistant varieties being considered the most appropriate method. However, little is known about the genes involved in the defense response of the plants to this anomaly. Considering that many physiological alterations associated with plant stress responses are controlled at a transcriptional level, in this study we sought the identification and characterization of the gene expression products differentially expressed in the response to the citrus blight. Through the suppressive subtractive hybridization technique, expressed cDNA libraries were built using mRNAs isolated from "Cravo" lemon tree roots (Citrus limonia L. Osbeck) under "Pera" orange (Citrus sinensis L. Osbeck) of healthy and sick plants. 129 clones were obtained by subtraction and their sequences were compared in databases. 34 of them linked to proteins associated to stress processes, while the others were similar to sequences of unknown functions or did not present similarity with sequences deposited in the databases. 3 genes were selected and their expressions were studied by RT -qPCR in real-time. Plants with citrus blight presented an increase of the expression level in two of those genes, suggesting that these can be directly involved with this anomaly.Index terms: Differential gene expression, subtractive hybridization, RT-qPCR. RESUMOO Brasil é o maior produtor de citros do mundo, sendo responsável por mais de 20% de sua produção. No entanto, a produção ainda é baixa, em decorrência de problemas fitossanitários, como o Declínio do Citros que é uma anomalia cuja causa ainda não foi determinada e, consequentemente, não existem medidas de controle para minimizar as perdas na produção. O uso de variedades resistentes é considerado como a medida de controle mais adequada. Contudo, pouco se conhece sobre os genes envolvidos na resposta de defesa das plantas a essa anomalia. Considerando que muitas alterações fisiológicas associadas com respostas a estresses em plantas são controladas em nível transcripcional, neste estudo objetivou-se a identificação e caracterização dos produtos de expressão gênica diferencialmente expressos na resposta ao Declínio dos Citros. Por meio da técnica de hibridação subtrativa supressiva, bibliotecas de cDNAs expressos foram construídas utilizando mRNAs isolados de raízes de limoeiro "Cravo" (Citrus limonia L. Osbeck) sob laranja "Pera" (Citrus sinensis L. Osbeck) de plantas sadias e doentes. Cento e vinte e nove clones foram obtidos por subtração e suas sequências foram comparadas em bancos de dados. Trinta e quatro delas relacionaram-se a proteínas associadas a processos de estresses, enquanto as outras foram similares a sequências de funções desconheci...

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Identification of 14-3-3-like protein in sugarcane (Saccharum officinarum)

Abstract: In a search of the sugarcane expressed sequence tag (SUCEST) database we located three full-length cDNAs

Cited by 4 publications

References 31 publications

Ethanol Extract of Black Sugarcane Decrease Ischemia Volume and Bax Expression In Rats’ Brain

Ethanol Extract of Black Sugarcane Decrease Ischemia Volume and Bax Expression In Rats’ Brain

Análise de seqüências expressas em raízes de cana-de-açúcar colonizadas por Glomus clarum

IDENTIFICATION AND QUANTIFICATION OF DIFFERENTIALLY EXPRESSED GENES ASSOCIATED WITH CITRUS BLIGHT (Citrus spp.)

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