Identification of 57 sweet orange cultivars using AFLP markers

Fang, Jinggui; Wang, C.; Yu, Hao; Li, X.; Cui, Song; Chen, J.

doi:10.1080/14620316.2009.11512569

Cited by 3 publications

(3 citation statements)

References 23 publications

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“…All cultivars exhibited some variation in traits such as fruit character, maturity time, and more. As indicated in the CCPP website (http://www.ccpp.ucr.edu/variety/navels.html) and some reports (Kahn et al 2007;Fang et al 2009). Total DNA was isolated from mixed samples of several leaves growing at different growing stages determined by leaf sizes, including the leaves about 1.5, 2.5, and 3.5 cm long, collected from two trees of each cultivar in May and November, 2005.…”

Section: Plant Materials and Dna Isolationmentioning

confidence: 98%

“…Although variation of agronomic traits among sweet orange adult trees can be obvious, genetic variability within cultivars is difficult to be detected by molecular markers (Bretò et al 2001;Fang and Roose 1997;Federici et al 1998;Herrero et al 1996;Fang et al 2009). In studies addressing the diversity of sweet orange cultivars (Fang et al 2009;Fang and Roose 1997), a combination of polymorphic amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers could not distinguish and individualize all the cultivars, and were not sufficient to account for the diversity suggested by the observed differences in agronomic traits. Thus, the diversity in agronomic traits of orange cultivars could be explained somehow by other kinds of non-sequence changes, such as DNA methylation or other epigenetic mechanisms that are involved in gene regulation or other mechanisms.…”

Section: Introductionmentioning

confidence: 99%

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Variation of cytosine methylation in 57 sweet orange cultivars

Fang

Cui

Qian³

et al. 2010

Acta Physiol Plant

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Sweet orange is an important group of citrus cultivars, which includes a number of bud sport cultivars. Little is known about the CpG methylation status of the CCGG sequences in the orange genome. In this study, methylation-sensitive amplification polymorphism (MSAP), based on the application of isoschizomers (Hpa II and Msp I), was first used to analyze cytosine methylation patterns in 57 orange cultivars that were not fully differentiated by regular DNA molecular markers. Three types of bands were generated from ten primer pairs. Type I bands were present following restriction with Eco RI ? Hpa II and Eco RI ? Msp I; type II or type III were present only following restriction with either Eco RI ? Hpa II or with Eco RI ? Msp I. The total number of these three types of bands was 802, 72, and 157, respectively. Among these, the number of polymorphic bands were 244 (30.2%), 23 (31.9%), and 32 (20.4%), in type I, II and III, respectively. The methylation patterns of these 57 cultivars are discussed and assessed by dendrograms derived from the analysis of polymorphic MSAP bands. The distribution of polymorphic bands of the above three types demonstrate the methylation patterns and frequency at the cytosine loci.We suggest that methylation events could be more frequent than demethylation events, and that the methylation patterns maybe associated with phenotypic traits.

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Section: Plant Materials and Dna Isolationmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Variation of cytosine methylation in 57 sweet orange cultivars

Fang

Cui

Qian³

et al. 2010

Acta Physiol Plant

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“…Exemples of the use of molecular markers in citrus are phylogenetic studies, germplasm characterization, distinction between zygotic and nucelar plantules, marker assisted selection and genetic mapping. For that moleculars markers used include among others: Isozymes (Roose, 1988;Torres et al, 1982Torres et al, , 1978, RAPDs (Randomly Amplified PolymorphicDNAs; (Luro et al, 1994;Xiao et al, 1995), ISSRs (Inter-Simple SequenceRepeats; (Fang et al, 1998, AFLPs (Amplified Fragment LengthPolymorphisms; (de Oliveira et al, 2007;Fang et al, 2009;Liang et al, 2006), RFLPs (Restriction Fragment Length Polymorphisms; (Durham et al, 1992;Federici et al, 2000Federici et al, , 1998Luro et al, 1996) and SSRs (Simple SequenceRepeats or microsatellites; (Barkley et al, 2006;Chen et al, 2006;Kijas et al, 1995;Liang et al, 2015;Liu et al, 2013;Luro et al, 2008;Ollitrault et al, 2010;.…”

Section: Breeding For Seedlessnessmentioning

confidence: 99%

Assessment of Citrus Reproductive Biology for Seedless Mandarin Production and its Interaction with Temperature

Resurrección¹

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Since the origin of citrus, ancestral natural hybridizations, thousands of years of cultivation and admixture phenomenon have resulted in the complexity of the reproductive biology of today's cultivated citrus, which often involves female and male sterility, self-incompatibility, parthenocarpy and apomixis in different degrees. The global objective of this PhD was to increase our knowledge on different reproductive aspects that are crucial for citrus breeding and propagation and their interaction with environmental conditions.

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