Identification of a C-type lectin with antiviral and antibacterial activity from pacific white shrimp Litopenaeus vannamei

Li, Ming; Li, Chaozheng; Ma, Chao; Li, Haoyang; Zuo, Hongliang; Weng, Shaoping; Chen, Xiaohan; Zeng, Dingyuan; He, Jianguo; Xu, Xiaopeng

doi:10.1016/j.dci.2014.04.014

Cited by 123 publications

(49 citation statements)

References 49 publications

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“…9). Similar findings have been reported for other lectin-related proteins in pacific white shrimp [42] and bay scallop [43]. Previous studies indicated that the agglutination activity of antiserum is inhibited by teichoic acid from the Gram-positive bacterial cell wall [44e46].…”

Section: Discussionsupporting

confidence: 82%

Functional characterisation and expression analysis of recombinant serum amyloid P isoform 1 (RbSAP1) from rock bream (Oplegnathus fasciatus)

Choi¹,

Shim²,

An³

et al. 2015

Fish & Shellfish Immunology

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Section: Discussionsupporting

confidence: 82%

Functional characterisation and expression analysis of recombinant serum amyloid P isoform 1 (RbSAP1) from rock bream (Oplegnathus fasciatus)

Choi¹,

Shim²,

An³

et al. 2015

Fish & Shellfish Immunology

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“…Deletion of the NF-κB binding site led to the abolishment of LvCTL3 expression, suggesting its regulation by NF-κB. This finding represents a first step towards the incorporation of CTLD proteins into the poorly understood immune signaling network in crustaceans [46] .…”

Section: Crustaceansmentioning

confidence: 86%

High Innate Immune Specificity through Diversified C-Type Lectin-Like Domain Proteins in Invertebrates

et al. 2015

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“…A growing number of CTLs have recently been discovered from marine invertebrates, especially crustaceans and molluscs, including those from F. chinensis [16,21,46,47], L. vannamei [15,19,45,48], Penaeus monodon [49,50], C. farreri [18,23,24,26], A. irradians [22,25] and Crassostrea gigas [51], Crassostrea virginica [52], and Haliotis discus discus [17]. Relatively little information is available on CTLs in echinoderms, and a handful of sea cucumber CTLs have been reported, such as MBL-C (Cucumaria japonica) [53], SJL-I (S. japonicus) [35,36], MBL-AJ and AJCTL (A. japonicus) [37,38].…”

Section: Discussionmentioning

confidence: 99%

“…An increasing number of CTLs from marine invertebrates (especially crustaceans and molluscs) have recently been reported to be involved in the innate immune response [15e19]. For example, three CTLs from shrimp Litopenaeus vannamei (LvLectin-1, LvLectin-2 and LvCTL3) played a role in the immune response toward bacterial and viral infections [15,19]. Five CTLs from shrimp Fenneropenaeus chinensis agglutinated bacterial pathogens [16,20,21].…”

Section: Introductionmentioning

confidence: 99%

Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

Wei

Liu

Yang

et al. 2015

Fish & Shellfish Immunology

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a b s t r a c t C-type lectin is one important pattern recognition receptor (PRR) that plays crucial roles in multiple immune responses. A C-type lectin from sea cucumber Apostichopus japonicus (AjCTL-1) was characterized in the present study. The amino acid sequence of AjCTL-1 shared high similarities with other Ctype lectins from invertebrates and vertebrates. The C-type lectin domain (CTLD) of AjCTL-1 contained a Ca 2þ -binding site 2 and four conserved cysteine residues. AjCTL-1 mRNA expression patterns in tissues and after bacterial challenge were then analysed. Quantitative PCR revealed that AjCTL-1 mRNA was widely expressed in the tested tissues of healthy sea cucumber. The highest expression level occurred in gonad followed by body wall, coelomocytes, tentacle, intestinum and longitudinal muscle, and the lowest expression level was in respiratory tree. AjCTL-1 mRNA expression in coelomocytes was significantly induced by gram-negative Listonella anguillarum and gram-positive Micrococcus luteus, with different upregulation patterns post-challenge. Recombinant AjCTL-1 exhibited the ability to bind peptidoglycan directly, agglutinate M. luteus, Staphylococcus aureus and Escherichia coli, in a Ca 2þ -dependant manner, and enhance the phagocytosis of coelomocytes against E. coli in vitro. The results indicated that AjCTL-1 could act as a PRR in Apostichopus japonicus and had critical roles in non-self recognition and bacterial clearance against invading microbes.

show abstract

Identification of a C-type lectin with antiviral and antibacterial activity from pacific white shrimp Litopenaeus vannamei

Cited by 123 publications

References 49 publications

Functional characterisation and expression analysis of recombinant serum amyloid P isoform 1 (RbSAP1) from rock bream (Oplegnathus fasciatus)

Functional characterisation and expression analysis of recombinant serum amyloid P isoform 1 (RbSAP1) from rock bream (Oplegnathus fasciatus)

High Innate Immune Specificity through Diversified C-Type Lectin-Like Domain Proteins in Invertebrates

Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

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