Identification of a caleosin associated with hazelnut (Corylus avellana L.) oil bodies

Abstract

Caleosins are involved in several cellular and biological processes that are closely associated with the synthesis, degradation and stability of oil bodies (OB).• Because of the importance and the multiple roles of these OB-associated proteins, in silico identification of sequences corresponding to putative caleosins in the hazelnut genome has been performed, and the association with seed OB was verified using a proteomic approach.• Five full-length sequences (CavCLO-H1, CavCLO-H2, CavCLO-H3, CavCLO-L1, CavCLO-L2), belonging to the two groups of caleosins (H and L), have been identified in the hazelnut genome. The number of identified caleosins is in agreement with that previously observed in other plant species, confirming that caleosins comprise small gene families in plants.• A proteomic approach allowed us to verify only the presence of CavCLO-H1 in hazelnut OB, suggesting that several members inside this family could have different roles during plant growth and development. In silico analysis also suggests that CavCLO-H1 may act as a peroxygenase.

OsClo5 functions as a transcriptional co‐repressor by interacting with OsDi19‐5 to negatively affect salt stress tolerance in rice seedlings

“…The lipid‐associated Ca 2+ binding ability also occurs in fungi and green algae (Naested et al ., 2000; Hanano et al ., 2006; Rahman et al ., 2018). Caleosin gene families have been annotated in castor bean and flax (Hyun et al ., 2013); wheat, barley, rye, rice and Brachypodium (Khalil et al ., 2014); Arabidopsis (Shen et al ., 2014); Brassicaceae (Shen et al ., 2016) and hazelnut (Lamberti et al ., 2020).…”

A computational study on the structure–function relationships of plant caleosins

“…Reviewing other research met our results and partly opened up the knot of this puzzle. Accordingly, most CN and CNC caleosins had been reported as seed-specific proteins 6,9,[30][31][32][33][34][35][36][37][38][39][40][41][42][43][44] , while NC members had been isolated from the floral parts 9,17,42,[45][46][47][48][49][50][51] . That might explain the higher portion of NC and CNC in gymnosperms and angiosperms, respectively (Fig.…”

Identification of Key Lipogenesis Stages and Proteins Involved in Walnut Kernel Development

“…12 In addition, proteomics has been considered a promising method in determining DEP levels, indicating the molecular pathways and interactions related to DEPs. 13 Label-free proteomics is a promising alternative method that does not require extra sample preparation and is less time-consuming and cost-effective and requires a low amount of sample compared to labeling techniques. 14 It has been widely used in seed development and foods for comparing protein abundance between two or more samples.…”

“…Caleosin may also participate in the storage lipid degradation of OBs. The disruption of caleosin could cause a decrease in fatty acid degradation …”

“…Proteomics can be employed in studies of protein expression levels, protein–protein interactions, and post-translation modifications . In addition, proteomics has been considered a promising method in determining DEP levels, indicating the molecular pathways and interactions related to DEPs . Label-free proteomics is a promising alternative method that does not require extra sample preparation and is less time-consuming and cost-effective and requires a low amount of sample compared to labeling techniques .…”