Identification of a candidate gene for Rc-D1, a locus controlling red coleoptile colour in wheat

Wang, Y.Q.; Hou, Xiao-Qiang; Zhang, B.; Chen, W.J.; Liu, D.C.; Liu, B.L.; Zhang, H.G.

doi:10.1556/0806.43.2015.030

Cited by 15 publications

(18 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…R and Rc occur on chromosomes 3 and 7, respectively (Himi et al 2011;Himi and Taketa 2015;Wang et al 2016), and Pp1 and Pp3, which control the purple grain trait, are located on chromosomes 7B and 2A, respectively (Khlestkina et al 2010). Therefore, the chromosomal site of TaMYB3 is clearly inconsistent with the chromosomal locations of the two complementary genes responsible for the purple grain trait of wheat.…”

Section: Discussionmentioning

confidence: 99%

“…Similar functional MYB regulators also exist in Arabidopsis (AtMYB75 [PAP1]) and AtMYB90 [PAP2]) (Borevitz et al 2000), petunia (AN2) (Quattrocchio et al 1999), sweet potato (MYB1) (Mano et al 2007), legumes (LAP1) (Peel et al 2009), and Epimedium sagitta tum (EsMYBA1) (Huang et al 2013). Among the genes associated with anthocyanin biosynthesis in Triticum aestivum, the red grain (R) and red coleoptile (Rc) genes have been shown to encode R2R3-MYB transcription factors that regulate proanthocyanidin and anthocyanin biosynthesis in grains and coleoptiles, respectively (Himi et al 2011;Himi and Taketa 2015;Wang et al 2016). The candidate gene for the purple pericarp trait in common wheat, Pp3, is the basic helix-loop-helix (bHLH) transcriptor TaMYC1 (Shoeva et al 2014;Liu et al 2016), and the Pp1 genes are thought to be orthologues of maize C1 and rice OsC1, which encode MYB-like transcription factors responsible for the activation of the structural genes encoding various enzymes that participate in anthocyanin synthesis (Saitoh et al 2004;Khlestkina 2013).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

TaMYB3, encoding a functional MYB transcriptor, isolated from the purple pericarp of Triticum aestivum

Zong

Liu

et al. 2017

Cereal Research Communications

Self Cite

View full text Add to dashboard Cite

Purple pericarp is an interesting and useful trait in Triticum aestivum, but the molecular mechanism behind this phenotype remains unclear. The allelic variation in the MYB transcriptors is associated with the phenotype of pigmented organs in many plants. In this study, a MYB transcription factor gene, TaMYB3, was isolated using homology-based cloning and a differentially expressed gene mining approach, to verify the function of the MYB transcriptor in the purple pericarp. The coding sequence of TaMYB3 in cultivar Gy115 was the same as that in cultivar Opata. TaMYB3 was localized to FL0.62-0.95 on chromosome 4BL. The TaMYB3 protein contains DNA-binding and transcription-activation domains, and clustered on a phylogenetic tree with the MYB proteins that regulates anthocyanin and proanthocyanin biosynthesis. TaMYB3 localized in the nuclei of Arabidopsis thaliana and wheat protoplasts after it was transiently expressed with PEG transformation. TaMYB3 induced anthocyanin synthesis in the pericarp cells of Opata in the dark in collaboration with the basic helix-loop-helix protein ZmR, which is also the function of ZmC1. However, TaMYB3 alone did not induce anthocyanin biosynthesis in the pericarp cells of the white grain wheat cultivar Opata in the light after bombardment, whereas the single protein ZmR did. Light increased the expression of TaMYB3 in the pericarp of Gy115 and Opata, but only induced anthocyanin biosynthesis in the grains of Gy115. Our results extend our understanding of the molecular mechanism of the purple pericarp trait in T. aestivum.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

TaMYB3, encoding a functional MYB transcriptor, isolated from the purple pericarp of Triticum aestivum

Zong

Liu

et al. 2017

Cereal Research Communications

Self Cite

View full text Add to dashboard Cite

show abstract

“…ZmC1 was the first MYB gene regulating anthocyanin biosynthesis to be isolated from maize (Cone et al 1986). ZmC1 possesses special DNA-binding and transcriptional domains for promoting the expression of the structural genes involved in anthocyanin biosynthesis (Grotewold et al 2000), and Ta-MYB-D1 is a candidate gene for Rc-D1 (Wang et al 2015). TaMYB-A1 had the same DNA-binding and regulatory activator domains as TaMYB-D1 and ZmC1, which indicated that it may up-regulate the structural genes of anthocyanin biosynthesis.…”

Section: Tamyb-a1 Was the Myb Transcription Factor Regulating Anthocymentioning

confidence: 98%

“…TaMYB-D1 has a regulatory function improving anthocyanin biosynthesis (Wang et al 2015). Thus, TaMYB-D1 was selected as the positive control for evaluating the function of TaMYB-A1.…”

Section: Functional Verification Of Tamyb-a1mentioning

confidence: 99%

“…After analysing the expression levels of structural genes in the coleoptiles of the above substitution lines, each dominant Rc allele was hypothesized to encode a transcriptional activator (Ahmed et al 2006). Recently, the candidate dominant Rc alleles were isolated from the short arms of chromosomes 7A, 7B, and 7D (Himi and Taketa 2015), and the candidate dominant TaMYB-D1 on the 7D chromosome was linked to the red coleoptile trait (Wang et al 2015). In this article, TaMYB-A1 and the corresponding loss-of-function allele TaMYB-a1 at chromosome 7A was isolated from T. aestivum and T. urartu, and their functions in regulating anthocyanin biosynthesis were verified.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Frame-shift mutation causes the function loss of TaMYB-A1 regulating anthocyanin biosynthesis in Triticum aestivum

Wei

Chen

et al. 2017

Cereal Research Communications

Self Cite

View full text Add to dashboard Cite

Red coleoptile is an easily observed trait in Triticum aestivum and can provide some protection against stress. Here, TaMYB-A1 or TuMYB-A1, homologous to TaMYB-D1, which controls red coleoptile formation in the common wheat cultivar 'Gy115', was isolated from eight T. aestivum and 34 T. urartu cultivars. The genome sequence of TaMYB-A1 was 867 bp with an intron of 93 bp, which was similar to the MYBs regulating anthocyanin biosynthesis in T. aestivum but different from other MYB transcription factors regulating anthocyanin biosynthesis. TaMYB-A1 had an integrated DNA-binding domain of 102 amino acids and a transcriptional domain of 42 amino acids, which was responsible for regulating anthocyanin biosynthesis. TaMYB-A1 was assigned to the same branch as the MYBs regulating anthocyanin biosynthesis in a phylogenetic tree. A transient expression analysis showed that TaMYB-A1 induced 'Opata' coleoptile cells to synthesize anthocyanin with the help of ZmR. A non-functional allele of TaMYB-a1 existed in common wheat cultivars containing rc-a1. One single nucleotide was deleted 715 bp after the start codon in TaMYB-a1 compared with TaMYB-A1. The deletion caused a frame shift mutation, destroyed the DNA transcription activator domain, and resulted in TaMYB-a1 losing its ability to regulate anthocyanin biosynthesis in 'Opata' coleoptile cells. Those cultivars with functional TaMYB-A1 or TuMYB-A1 have red coleoptiles. The isolation of TaMYB-A1 should aid in understanding the molecular mechanisms of coleoptile traits in T. aestivum.

show abstract

A MYB family transcription factor TdRCA1 from wild emmer wheat regulates anthocyanin biosynthesis in coleoptile

Li,

Zhang,

et al. 2024

Theor Appl Genet

View full text Add to dashboard Cite

Identification of a candidate gene for Rc-D1, a locus controlling red coleoptile colour in wheat

Cited by 15 publications

References 33 publications

TaMYB3, encoding a functional MYB transcriptor, isolated from the purple pericarp of Triticum aestivum

TaMYB3, encoding a functional MYB transcriptor, isolated from the purple pericarp of Triticum aestivum

Frame-shift mutation causes the function loss of TaMYB-A1 regulating anthocyanin biosynthesis in Triticum aestivum

A MYB family transcription factor TdRCA1 from wild emmer wheat regulates anthocyanin biosynthesis in coleoptile

Contact Info

Product

Resources

About