Identification of a cDNA Clone Coding for a Novel Calcium-Binding Protein from Potato Tuber

Gellatly, Kevin S.; Lefebvre, Daniel D.

doi:10.1104/pp.101.4.1405

Cited by 6 publications

(2 citation statements)

References 5 publications

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“…Two other previously identified CML proteins with significant similarity to APR134 are CAST from potato (Gellatly and Lefebvre, 1993) and ACRE-31 from tobacco (Durrant et al, 2000). Interestingly, the ACRE-31 gene was previously shown to be induced during the Avr9/Cf9-mediated defense response in tobacco (Durrant et al, 2000) although its function was not investigated.…”

Section: Identification Of Arabidopsis Cmlsmentioning

confidence: 95%

Calmodulin-like Proteins from Arabidopsis and Tomato are Involved in Host Defense Against Pseudomonas syringae pv. tomato

et al. 2005

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Complex signal transduction pathways underlie the myriad plant responses to attack by pathogens. Ca(2+) is a universal second messenger in eukaryotes that modulates various signal transduction pathways through stimulus-specific changes in its intracellular concentration. Ca(2+)-binding proteins such as calmodulin (CaM) detect Ca(2+) signals and regulate downstream targets as part of a coordinated cellular response to a given stimulus. Here we report the characterization of a tomato gene (APR134) encoding a CaM-related protein that is induced in disease-resistant leaves in response to attack by Pseudomonas syringae pv. tomato. We show that suppression of APR134 gene expression in tomato (Solanum lycopersicum), using virus-induced gene silencing (VIGS), compromises the plant's immune response. We isolated APR134-like genes from Arabidopsis, termed CML42 and CML43, to investigate whether they serve a functionally similar role. Gene expression analysis revealed that CML43 is rapidly induced in disease-resistant Arabidopsis leaves following inoculation with Pseudomonas syringae pv. tomato. Overexpression of CML43 in Arabidopsis accelerated the hypersensitive response. Recombinant APR134, CML42, and CML43 proteins all bind Ca(2+ )in vitro. Collectively, our data support a role for CML43, and APR134 as important mediators of Ca(2+)-dependent signals during the plant immune response to bacterial pathogens.

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Section: Identification Of Arabidopsis Cmlsmentioning

confidence: 95%

Calmodulin-like Proteins from Arabidopsis and Tomato are Involved in Host Defense Against Pseudomonas syringae pv. tomato

et al. 2005

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“…ACRE-31 is 83% identical to CAST, a calcium binding protein of unknown function from potato (Gellatly and Lefebvre, 1993). It has three putative EF hand calcium binding domains.…”

Section: Isolation Of Cdna Clones Corresponding To 13 Cdna-aflp Fragmmentioning

confidence: 99%

cDNA-AFLP Reveals a Striking Overlap in Race-Specific Resistance and Wound Response Gene Expression Profiles

Durrant¹,

Rowland²,

Piedras³

et al. 2000

The Plant Cell

129

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The tomato Cf-9 gene confers resistance to races of the fungal pathogen Cladosporium fulvum expressing the Avr9 gene. cDNA amplified fragment length polymorphism analysis was used to display transcripts whose expression is rapidly altered during the Avr9- and Cf-9-mediated defense response in tobacco cell cultures. Diphenyleneiodonium was used to abolish the production of active oxygen species during gene induction. Of 30,000 fragments inspected, 290 showed altered abundance, of which 263 were induced independently of active oxygen species. cDNA clones were obtained for 13 ACRE (for Avr9/Cf-9 rapidly elicited) genes. ACRE gene induction occurred in the presence of cycloheximide. Avr9 induced ACRE gene expression in leaves. Surprisingly, ACRE genes were also rapidly but transiently induced in leaves in response to other stresses. The amino acid sequences of some ACRE proteins are homologous to sequences of known proteins such as ethylene response element binding protein transcription factors, the N resistance protein, a calcium binding protein, 13-lipoxygenase, and a RING-H2 zinc finger protein. Rapid induction of ACRE genes suggests that they play a pivotal role during plant defense responses.

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2010

Calcium Binding Proteins

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Identification of a cDNA Clone Coding for a Novel Calcium-Binding Protein from Potato Tuber

Cited by 6 publications

References 5 publications

Calmodulin-like Proteins from Arabidopsis and Tomato are Involved in Host Defense Against Pseudomonas syringae pv. tomato

Calmodulin-like Proteins from Arabidopsis and Tomato are Involved in Host Defense Against Pseudomonas syringae pv. tomato

cDNA-AFLP Reveals a Striking Overlap in Race-Specific Resistance and Wound Response Gene Expression Profiles

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