Identification of a chicken ( Gallus gallus ) endogenous reference gene ( Actb ) and its application in meat adulteration

Xiang, Wenjin; Shang, Ying; Wang, Qin; Xu, Yuancong; Zhu, Pengyu; Huang, Kunlun; Xu, Wentao

doi:10.1016/j.foodchem.2017.05.038

Cited by 27 publications

(14 citation statements)

References 28 publications

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“…SYBR Green PCR SuperMix (Bio-Rad, Hercules, CA, USA) and a Bio-Rad CFX96 real-time PCR detection system (Bio-Rad, Hercules, CA, USA) were used for RT-PCR, and each sample was assayed three times. β-actin [24] was used as a housekeeping gene. The 2 −∆∆CT method was used for normalization of the qPCR results, after which the normalized data was used for statistical analysis, and p < 0.05 was considered significantly different.…”

Section: Rt-qpcr Validation Of the De Lncrnas And Their Target Genesmentioning

confidence: 99%

Comparative Transcriptome Analysis Suggests Key Roles for 5-Hydroxytryptamlne Receptors in Control of Goose Egg Production

Ouyang

Wang

et al. 2020

Genes

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To date, research on poultry egg production performance has only been conducted within inter or intra-breed groups, while those combining both inter- and intra-breed groups are lacking. Egg production performance is known to differ markedly between Sichuan white goose (Anser cygnoides) and Landes goose (Anser anser). In order to understand the mechanism of egg production performance in geese, we undertook this study. Here, 18 ovarian stromal samples from both Sichuan white goose and Landes goose at the age of 145 days (3 individuals before egg production initiation for each breed) and 730 days (3 high- and low egg production individuals during non-laying periods for each breed) were collected to reveal the genome-wide expression profiles of ovarian mRNAs and lncRNAs between these two geese breeds at different physiological stages. Briefly, 58, 347, 797, 777, and 881 differentially expressed genes (DEGs) and 56, 24, 154, 105, and 224 differentially expressed long non-coding RNAs (DElncRNAs) were found in LLD vs. HLD (low egg production Landes goose vs. high egg production Landes goose), LSC vs. HSC (low egg production Sichuan White goose vs. high egg production Sichuan white goose), YLD vs. YSC (young Landes goose vs. young Sichuan white goose), HLD vs. HSC (high egg production Landes goose vs. high egg production Sichuan white goose), and LLD vs. LSC (low egg production Landes goose vs. low egg production Sichuan white goose) groups, respectively. Functional enrichment analysis of these DEGs and DElncRNAs suggest that the “neuroactive ligand–receptor interaction pathway” is crucial for egg production, and particularly, members of the 5-hydroxytryptamine receptor (HTR) family affect egg production by regulating ovarian metabolic function. Furthermore, the big differences in the secondary structures among HTR1F and HTR1B, HTR2B, and HTR7 may lead to their different expression patterns in goose ovaries of both inter- and intra-breed groups. These results provide novel insights into the mechanisms regulating poultry egg production performance.

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Section: Rt-qpcr Validation Of the De Lncrnas And Their Target Genesmentioning

confidence: 99%

Comparative Transcriptome Analysis Suggests Key Roles for 5-Hydroxytryptamlne Receptors in Control of Goose Egg Production

Ouyang

Wang

et al. 2020

Genes

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“…Most of them are single real-time PCR systems to detect a mitochondrial gene like cytochrome b [4,23,27,28,35,36]. For the detection of chicken meat, a few chromosomal genes are used like interleukin-2 gene [37] or β-actin gene [38]. Fewer real-time PCR systems have been published for the detection of meat from guinea fowl [39], pheasant [35,39], or quail [35,39].…”

Section: Discussionmentioning

confidence: 99%

Comparison of Real-Time PCR Quantification Methods in the Identification of Poultry Species in Meat Products

Dolch

Andrée

Schwägele

2020

Foods

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Poultry meat is consumed worldwide and is prone to food fraud because of large price differences among meat from different poultry species. Precise and sensitive analytical methods are necessary to control poultry meat products. We chose species–specific sequences of the cytochrome b gene to develop two multiplex real-time polymerase chain reaction (real-time PCR) systems: one for chicken (Gallus gallus), guinea fowl (Numida meleagris), and pheasant (Phasianus colchicus), and one for quail (Coturnix japonica) and turkey (Meleagris gallopavo). For each species, added meat could be detected down to 0.5 % w/w. No cross reactions were seen. For these two real-time PCR systems, we applied three different quantification methods: (A) with relative standard curves, (B) with matrix-specific multiplication factors, and (C) with an internal DNA reference sequence to normalize and to control inhibition. All three quantification methods had reasonable recovery rates from 43% to 173%. Method B had more accepted recovery rates, i.e., in the range 70–130%, namely 83% compared to 75% for method A or C.

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“…Dn contrast to proteins, is DNA mostly steady at high temperatures; so it can be analyzed in processed, degraded and mixed supplies (Lenstra, 2003;Meira et al, 2017;Abbas et al, 2018). On addition, while the discovery and identification of proteins rely on the type of the tissue examined, DNA exists and is typical in nearly all cells also the variety given by the hereditary code allows the isolation of even entirely-related species (Lockley & Bardsley, 2000;Ballin, 2010;Xiang et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…DNA-based strategies that have been genuine for species recognizable pieces of proof incorporate DNA hybridization, polymerase chain reaction (PCR), multiplex-PCR, species-specific PCR, restriction-fragment-length-polymorphism (PCR-RFLP) test, real-time PCR and PCR sequencing (Lopez-Andreo et al, 2005;Man et al, 2007;Karlsson & Holmlund, 2007;Chen et al, 2010;Xiang et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Species – specific PCR test for the quick recognition of equine tissue in raw and processed beef meat mixtures

et al. 2019

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PCR was applied for the discovery of adulteration of crude and processed beef meat with horse and donkey tissue. This was performed by blending (w/w) horse or donkey meat to beef meat in an extent up to 1:10000 (0.01%). The sensitivity was resolved as high as 0.01%. All used primers showed specificity in the PCR reactions utilizing layout DNAs from three animal species. PCR application on 96 beef meat and meat product samples gathered randomly from street vendors and prominent retail markets (24 of burger, 16 of minced meat, 24 of kofta, 16 of sausage, 7 of raw meat and 9 of launcheon) uncovered 6 positive for donkey tissue (3 from sausage, 2 from minced meat and 1 from kofta) and 2 positive for horse tissue (from sausage). This basic PCR strategy effectively distinguished adulteration of raw and processed beef meat samples with horse and donkey tissue. This work also highlights on the severity of the meat adulteration problem in Egypt.

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Identification of a chicken ( Gallus gallus ) endogenous reference gene ( Actb ) and its application in meat adulteration

Cited by 27 publications

References 28 publications

Comparative Transcriptome Analysis Suggests Key Roles for 5-Hydroxytryptamlne Receptors in Control of Goose Egg Production

Comparative Transcriptome Analysis Suggests Key Roles for 5-Hydroxytryptamlne Receptors in Control of Goose Egg Production

Comparison of Real-Time PCR Quantification Methods in the Identification of Poultry Species in Meat Products

Species – specific PCR test for the quick recognition of equine tissue in raw and processed beef meat mixtures

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