Identification of a cisplatin resistant-based prognostic immune related gene signature in MIBC

Wu, Yunfei; Xu, Zhijie; Fu, Guanghou; Chen, Xiaoyi; Tian, Junjie; Cai, Hairong; Jiang, Peng; Jin, Baiye

doi:10.1016/j.tranon.2024.101942

Translational Oncology

2024

DOI: 10.1016/j.tranon.2024.101942

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Identification of a cisplatin resistant-based prognostic immune related gene signature in MIBC

Yunfei Wu,

Zhijie Xu,

Guanghou Fu

et al.

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Silencing CCT3 induces ferroptosis through the NOD1-NF-κB signaling pathway in bladder cancer

Huang,

Luo,

Wang

et al. 2024

Sci Rep

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Bladder cancer (BCa) is a lethal malignancy of the urinary system and exhibits a poor prognosis. Chaperonin-containing tailless complex polypeptide 1 subunit 3 (CCT3) acts as an oncogene in various tumors, whereas its effect on BCa remains unknown. We identified the ferroptosis-associated differentially expressed genes through bioinformatic analysis and selected CCT3 for further verification. The levels of cell viability, apoptosis, migration, invasion, and proliferation were measured to clarify the effect of silencing CCT3 on BCa cells. Then we evaluated the role of CCT3 knockdown in vivo. Ferroptosis was assessed by the expression detection of the ferroptosis-related proteins. The underlying mechanism was predicted by RNA sequencing and verified by an agonist for nucleotide-binding and oligomerization domain 1 (NOD1). Western blotting was conducted to detect the protein expression of NOD1, nuclear factor kappa B (NF-κB) inhibitor alpha (IκBα), and phospho-IκBα (p-IκBα). In vitro, down-regulation of CCT3 suppressed the cell viability, migration, invasion, and proliferation, as well as induced apoptosis of BCa cells. In vivo, silencing CCT3 elevated the body weight of mice and suppressed the BCa progression. In addition, CCT3 knockdown could induce ferroptosis in vitro and in vivo. CCT3 knockdown suppressed the expression of NOD1 and p-IκBα/IκBα and the NOD1 agonist could reverse the effect of CCT3 suppression on BCa in vitro and in vivo. In summary, our findings demonstrate that silencing CCT3 inhibits BCa via induction of ferroptosis and suppression of the NOD1-NF-κB pathway. Supplementary Information The online version contains supplementary material available at 10.1038/s41598-024-76724-z.

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Silencing CCT3 induces ferroptosis through the NOD1-NF-κB signaling pathway in bladder cancer

Huang,

Luo,

Wang

et al. 2024

Sci Rep

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Identification of a cisplatin resistant-based prognostic immune related gene signature in MIBC

Cited by 1 publication

References 55 publications

Silencing CCT3 induces ferroptosis through the NOD1-NF-κB signaling pathway in bladder cancer

Silencing CCT3 induces ferroptosis through the NOD1-NF-κB signaling pathway in bladder cancer

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