Identification of a conserved cluster of skin-specific genes encoding secreted proteins

Moffatt, Pierre; Salois, Patrick; St‐Amant, Natalie; Gaumond, Marie‐Hélène; Lanctôt, Christian

doi:10.1016/j.gene.2004.03.010

Cited by 38 publications

(61 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cells were washed and incubated with serum-free media for 72 h. Conditioned media were collected at 24 and 72 h, precipitated with trichloroacetic acid (fi nal concentration 10 % (v/v)), processed for Western blot analysis under denaturing conditions (Moffatt et al, 2004) and separated on a Ready Gel ® precast 15 % Tris-HCl polyacrylamide gels . Proteins were transferred onto 0.45 μm nitrocellulose membranes and probed with AMBN antibody (1:2,000), all in PBS-0.05 % Tween 20 (Fisher Scientifi c, Whitby, ON, Canada) with 5 % skim milk.…”

Section: Detection Of Ambn In Western Blottingmentioning

confidence: 99%

Ameloblastin is not implicated in bone remodelling and repair

Kuroda

Wazen²,

Sellin³

et al. 2011

eCM

View full text Add to dashboard Cite

Ameloblastin (AMBN) is an enamel matrix protein produced by ameloblasts. It has been suggested that AMBN might also be implicated in craniofacial bone formation. Our objective was to determine whether AMBN has an effect on osteogenic mineralisation and infl uences bone remodelling and repair. MC3T3-E1 cells were screened for endogenous expression of enamel proteins using real time PCR. Various osteogenic cells were infected with lentivirus encoding for AMBN and protein expression was verifi ed using immunochemistry. Cultures were stained with alizarin red and mineralisation was quantifi ed. Healing bone was probed for expression of AMBN by DNA microarray analysis. Tooth extraction, experimental tooth movement (ETM), and creation of a non-critical size bone defect in the tibia (BDT) were carried out in wild type and AMBN Δ5-6 mutant mice. Tissues were processed for immunolabelling of AMBN and Bril, an osteoblast specifi c protein associated with active bone formation. MC3T3-E1 cells and healing bone showed no significant expression of AMBN. Overexpression of AMBN in osteogenic cultures induced no noticeable changes in mineralisation. In wild type mice, AMBN was immunodetected in ameloblasts and enamel, but not in normal bone, and at sites where bone remodelling and repair were induced. Bone remodelling during ETM and BDT repair in AMBN Δ5-6 mice were not signifi cantly different from that in wild type animals. Our results suggest that AMBN does not infl uence osteogenic activity in vitro under the conditions used, and does not participate in craniofacial bone remodelling under mechanical stress and in repair of non-critical size bone defects.

show abstract

Section: Detection Of Ambn In Western Blottingmentioning

confidence: 99%

Ameloblastin is not implicated in bone remodelling and repair

Kuroda

Wazen²,

Sellin³

et al. 2011

eCM

View full text Add to dashboard Cite

show abstract

“…2); the ␤-glucosylceramidase involved in the maturation of glucosylceramides to ceramides in the stratum corneum; and two proteins with unknown function, dermokine ␤ and suprabasin (52).…”

Section: Clip-170 Association With Lamellar Bodies Of Human Epidermismentioning

confidence: 99%

Lamellar Bodies of Human Epidermis

Raymond

Peredo

Stella

et al. 2008

Molecular & Cellular Proteomics

View full text Add to dashboard Cite

“…Previously, we and Moffat et al [5,6] have identified dermokine (DK)-a/-b (sk30/89) as a novel keratinocyte-secreted peptide, as well as identifying the formation of a new stratified epitheliumsecreted gene complex (SCC) with two other keratinocytesecreted peptides, Kdap and suprabasin. DK has been reported to have several other isoforms, in addition to the a and b isoforms, including c1 and 2, d1-6, and e1-3 ( Fig.…”

Section: Introductionmentioning

confidence: 99%