Identification of a distinct strain of <i>Cotton leaf curl Gezira virus</i> infecting tomato in Oman

Shihi, Adel A. Al; Al-Sadi, Abdullah M.; Deadman, M. L.; Briddon, R. W.; Shahid, Muhammad

doi:10.1111/jph.12676

Cited by 14 publications

(3 citation statements)

References 40 publications

(65 reference statements)

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“…The latter introduced viruses, CLCuGeV, MYMIV, SLCV, and WmCSV, are endemic to Asia, the Middle East, Africa, and the Americas, respectively. There is knowledge and evidence of their transmission in Oman by the predicted, non-native NAFME 5 B. tabaci [ 1 , 47 , 48 ] and in other locations worldwide, where it has been established only recently following its introduction [ 3 ]. This scenario does not support the paradigm that B. tabaci cryptic species vector competency and a predicted co-evolution with begomoviral species are linked.…”

Section: Discussionmentioning

confidence: 99%

Native and Non-Native Bemisia tabaci NAFME Haplotypes Can Be Implicated in Dispersal of Endemic and Introduced Begomoviruses in Oman

Shahid

Paredes‐Montero

Ashfaq

et al. 2023

Insects

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Irrigated agriculture and global trade expansion have facilitated diversification and spread of begomoviruses (Geminiviridae), transmitted by the Bemisia tabaci (Gennadius) cryptic species. Oman is situated on major crossroads between Africa and South Asia, where endemic/native and introduced/exotic begomoviruses occur in agroecosystems. The B. tabaci ‘B mitotype’ belongs to the North Africa–Middle East (NAFME) cryptic species, comprising at least eight endemic haplotypes, of which haplotypes 6 and/or 8 are recognized invasives. Prevalence and associations among native and exotic begomoviruses and NAFME haplotypes in Oman were investigated. Nine begomoviral species were identified from B. tabaci infesting crop or wild plant species, with 67% and 33% representing native and exotic species, respectively. Haplotypes 2, 3, and 5 represented 31%, 3%, and 66% of the B. tabaci population, respectively. Logistic regression and correspondence analyses predicted ‘strong’- and ‘close’ virus–vector associations involving haplotypes 5 and 2 and the exotic chili leaf curl virus (ChiLCV) and endemic tomato yellow leaf curl virus-OM, respectively. Patterns favor a hypothesis of relaxed virus–vector specificity between an endemic haplotype and the introduced ChiLCV, whereas the endemic co-evolved TYLCV-OM and haplotype 2 virus–vector relationship was reinforced. Thus, in Oman, at least one native haplotype can facilitate the spread of endemic and introduced begomoviruses.

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Section: Discussionmentioning

confidence: 99%

Native and Non-Native Bemisia tabaci NAFME Haplotypes Can Be Implicated in Dispersal of Endemic and Introduced Begomoviruses in Oman

Shahid

Paredes‐Montero

Ashfaq

et al. 2023

Insects

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“…Interestingly, the CLCuGeV isolates from Saudi Arabia were separately clustered with the CLCuGeV isolates reported from Asia and more closely grouped with the CLCuGeV isolates from Pakistan and Iran. CLCuGeV is an African Malvaceae -adapted monopartite begomovirus, and now it has spread into the Indo–Pak subcontinent, the Middle East [ 19 , 34 , 35 ], and has recently been identified in the USA [ 36 ]. In Saudi Arabia, CLCuGeV has been reported earlier from okra [ 19 ]; however, the identification of CLCuGeV isolates from new crop hosts is alarming and may lead to widespread crop infection.…”

Section: Discussionmentioning

confidence: 99%

High-Throughput Sequencing Identified Distinct Bipartite and Monopartite Begomovirus Variants Associated with DNA-Satellites from Tomato and Muskmelon Plants in Saudi Arabia

Alhudaib

Almaghasla

El-Ganainy

et al. 2022

Plants

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The studies on the prevalence and genetic diversity of begomoviruses in Saudi Arabia are minimal. In this study, field-grown symptomatic tomato and muskmelon plants were collected, and initially, begomovirus infection was confirmed by the core coat protein sequences. Four tomato and two muskmelon plants with viral infections were further evaluated for Illumina MiSeq sequencing, and twelve sequences (2.7–2.8 kb) equivalent to the full-length DNA-A or DNA-B components of begomoviruses were obtained along with eight sequences (~1.3–1.4 kb) equivalent to the begomovirus-associated DNA-satellite components. Four begomovirus sequences obtained from tomato plants were variants of tomato yellow leaf curl virus (TYLCV) with nt sequence identities of 95.3–100%. Additionally, two tomato plants showed a mixed infection of TYLCV and cotton leaf curl Gezira virus (CLCuGeV), okra yellow crinkle Cameroon alphasatellite (OYCrCMA), and okra leaf curl Oman betasatellite (OLCuOMB). Meanwhile, from muskmelon plants, two sequences were closely related (99–99.6%) to the tomato leaf curl Palampur virus (ToLCPalV) DNA-A, whereas two other sequences showed 97.9-100% sequence identities to DNA-B of ToLCPalV, respectively. Complete genome sequences of CLCuGeV and associated DNA-satellites were also obtained from these muskmelon plants. The nt sequence identities of the CLCuGeV, OYCrCMA, and OLCuOMB isolates obtained were 98.3–100%, 99.5–100%, and 95.6–99.7% with their respective available variants. The recombination was only detected in TYLCV and OLCuOMB isolates. To our knowledge, this is the first identification of a mixed infection of bipartite and monopartite begomoviruses associated with DNA-satellites from tomato and muskmelon in Saudi Arabia. The begomovirus variants reported in this study were clustered with Iranian isolates of respective begomovirus components in the phylogenetic dendrogram. Thus, the Iranian agroecological route can be a possible introduction of these begomoviruses and/or their associated DNA-satellites into Saudi Arabia.

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“…Begomoviruses in Oman have been the subject of limited diversity, epidemiological exploration, and gene function studies, with only a handful of reports documenting exotic strains [10][11][12]. In the present study, we aimed to fill this gap by investigating the introduction of a new begomovirus, AlYVV, infecting Ageratum conyzoides in Oman.…”

Section: Introductionmentioning

confidence: 99%

Molecular Signature of a Novel Alternanthera Yellow Vein Virus Variant Infecting the Ageratum conyzoides Weed in Oman

Shafiq,

Ondrasek,

Al-Sadi

et al. 2023

Viruses

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Alternanthera yellow vein virus (AlYVV), a monopartite begomovirus, has been identified infecting a diverse range of crops and native plants in Pakistan, India, and China. However, distinctive yellow vein symptoms, characteristic of begomovirus infection, were observed on the Ageratum conyzoides weed in Oman, prompting a thorough genomic characterization in this study. The results unveiled a complete genome sequence of 2745 base pairs and an associated betasatellite spanning 1345 base pairs. In addition, Sequence Demarcation Tool analyses indicated the highest nucleotide identity of 92.8% with a previously reported AlYVV-[IN_abalpur_A_17:LC316182] strain, whereas the betasatellite exhibited a 99.8% nucleotide identity with isolates of tomato leaf curl betasatellite. Thus, our findings propose a novel AlYVV Oman virus (AlYVV-OM) variant, emphasizing the need for additional epidemiological surveillance to understand its prevalence and significance in Oman and the broader region. To effectively manage the spread of AlYVV-OM and minimize its potential harm to (agro)ecosystems, future research should focus on elucidating the genetic diversity of AlYVV-OM and its interactions with other begomoviruses.

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Identification of a distinct strain of Cotton leaf curl Gezira virus infecting tomato in Oman

Cited by 14 publications

References 40 publications

Native and Non-Native Bemisia tabaci NAFME Haplotypes Can Be Implicated in Dispersal of Endemic and Introduced Begomoviruses in Oman

Native and Non-Native Bemisia tabaci NAFME Haplotypes Can Be Implicated in Dispersal of Endemic and Introduced Begomoviruses in Oman

High-Throughput Sequencing Identified Distinct Bipartite and Monopartite Begomovirus Variants Associated with DNA-Satellites from Tomato and Muskmelon Plants in Saudi Arabia

Molecular Signature of a Novel Alternanthera Yellow Vein Virus Variant Infecting the Ageratum conyzoides Weed in Oman

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