Identification of a factor IX point mutation using SSCP analysis and direct sequencing

Demers, Daniel B.; Odelberg, Shannon J.; Fisher, LD

doi:10.1093/nar/18.18.5575

Cited by 19 publications

(1 citation statement)

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“…VR1 is the target for many of the serosubtyping antibodies, and considerable information concerning sequence variation at this locus is available (15,16,25). PCR amplification was followed by SSCP analysis, which is capable of highly sensitive detection of point mutations in DNA (7,10,26). PCR-SSCP analysis was used to demonstrate both the identities and nonidentities of meningococcal strains from clinical specimens and between clinical specimens and cultured strains.…”

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confidence: 99%

PCR-single-stranded confirmational polymorphism analysis for non-culture-based subtyping of meningococcal strains in clinical specimens

et al. 1997

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Subspecific typing of clinical meningococcal strains is important in the investigation of outbreaks and for disease surveillance. Serogrouping, typing, and subtyping of strains currently require isolation of a meningococcus from one or more clinical specimens. However, the increasing widespread practice of preadmission administration of parenteral antibiotics has resulted in a decrease in the frequency of positive cultures obtained from blood and cerebrospinal fluid. Confirmation of meningococcal disease can be obtained by meningococcus-specific PCR from both cerebrospinal fluid (H.

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confidence: 99%