Identification of a grpE Heat-shock Gene Homolog in the Archeon Methanosarcina mazie

Macario, Everly Conway de; Dugan, Charles B.; Macario, Alberto J. L.

doi:10.1006/jmbi.1994.1422

Cited by 38 publications

(23 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The genes that are present in the cloned fragment occur in the following order: clpB (1.5 kb; partial)-o$A (1.0 kb)-grpE (0.62 kb)-hsp70 (1.8 kb)-dnd (-0.1 kb; partial). The observed organization of these genes in M. capricolum is similar to the organization seen in several gram-positive bacteria (viz., Bacillus subtilis [31], Clostridium acetobuglicum [22], Lactococcus lactis [7], and Staphylococcus aureus [24]) and archaebacteria (viz., Methanosarcina mazei [19);]). In gram-positive bacteria such as B. subtilis, the last four genes (i.e., orjtA-grpE-hsp70 [dnaK]-dnaJ) form an operon (31), whereas in the gram-negative eubacteria (e.g., E. coli), an or$A gene homolog has not been identified.…”

Section: Discussionmentioning

confidence: 99%

“…The two mycoplasma species again showed a strong affinity for the low-G+ C-content gram-positive bacteria, and the bootstrap score of this branching (96 of 100) indicated that this relationship was robust. In the parsimony tree the closest affiliation of the mycoplasma species was with Erysipelothrix rhusiopathiae, which is a human and animal pathogen similar to many mycoplasma species (19). Another difference between the neighbor-joining and parsimony trees is the difference in the branching positions of the eukaryotic cytosolic homologs.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Phylogenetic Analysis of Mycoplasmas Based on Hsp70 Sequences: Cloning of the dnaK (hsp70) Gene Region of Mycoplasma capricolum

Falah

Gupta²

1997

International Journal of Systematic Bacteriology

View full text Add to dashboard Cite

A 5.0-kb region containing the hsp70 (dnaK) gene was cloned from Mycoplasmu capricolum and sequenced. In addition to the hsp70 gene, this sequence region also contained the complete sequences for the grpE and orfA genes and partial sequences for the clpB and dnaJ genes. The order of the above gene sequences in the cloned fragment was found to be clpB-orfA-~pE-hsp70-dn~, which is similar to the order seen in various other gram-positive groups of bacteria. The Hsp70 homologs from two mycoplasma species, Mycoplasma capricolum and Mycoplasma genitalium, contain a number of sequence signatures, including the absence of a large insert in the N-terminal quadrant, that are characteristics of the homologs from gram-positive bacteria and archaebacteria. A detailed phylogenetic analysis based on Hsp70 sequences was also performed. In neighbor-joining and parsimony trees based on Hsp70 sequences, both mycoplasma species branched with the low-G+ C-content gram-positive group of bacteria (e.g., Lactobacillus and Erysipelothrix species) in 87% and 96% of the bootstrap replicates, respectively, indicating their close evolutionary relationship to this group. The phylogenetic trees based on Hsp70 sequences show a polyphyletic branching of archaebacteria with the gram-positive species, which is statistically strongly favored.Mycoplasma species are the smallest free-living microorganisms known. They lack a typical eubacterial cell wall, periplasmic space, and an outer membrane (20,26,27). Their genomes are far smaller than the typical genomes of prokaryotes (they are one-sixth to one-third the size of the Escherichia cofi genome) and approach the theoretical size limit for genomes of free-living organisms (1 1, 20, 27). Mycoplasma species are distinct because of their genome size and their strict requirement for exogenous sterol (20,26). The peculiar characteristics of rnycoplasma species have aroused considerable interest concerning the evolution of these organisms. Phylogenetic studies based on 16s rRNA sequence data have indicated that the mycoplasmas branch with the low-G+C-content subgroup of gram-positive eubacteria, namely, the Lactobacillus group (20,30,32). Due to the limited genome size, many gene functions are absent from mycoplasmas; however, heat shock treatment of Mycopfusma capiicolum cells has been reported to cause increased synthesis of seven distinct protein bands, including a 70-kDa protein which reacts with antibodies to the E. coli DnaK protein (5). In our recent work we have been utilizing sequence data for the Hsp70 family of proteins to examine the evolutionary relationships among different groups of species (9, 14-16). Because of their large size and high degree of sequence conservation (the most conserved proteins found in any species) the Hsp70 proteins are well suited for examining deep phylogenetic relationships. Phylogenetic analyses based on Hsp70 sequences and several other protein sequences have revealed a number of important differences from rRNA-based phylogenies (1,2,4,12,14,15,29). In particula...

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Phylogenetic Analysis of Mycoplasmas Based on Hsp70 Sequences: Cloning of the dnaK (hsp70) Gene Region of Mycoplasma capricolum

Falah

Gupta²

1997

International Journal of Systematic Bacteriology

View full text Add to dashboard Cite

show abstract

“…Org., organism; perm., permease; RNAP, RNA polymerase; k-ase, kinase; RNaseP, RNaseP RNA. The sequence sources were the genome Web sites (see Web Site References in Supplemental Material, Table S3, online) and Conway de Macario et al 1994, Gupta and Singh 1993, Macario et al 1991, 1993, 1995, Hoffman-Bang et al 1999, Deppenmeir et al 2002, Galagan et al 2002, Smith et al 1997 , and A.S. Kazi and C.K.K. Nair, Bombay, India (accession number AF069527; gi, 10798841; H. mediterranei).…”

Section: Dnak Locus I Types and Dnak Phylogenymentioning

confidence: 99%

“…The dnaK locus first described for an archaeon was organized 5¢-hsp16-grpE-dnaK-dnaJ-trkA-3¢ (Conway de Macario et al 1994;, suggesting that this gene arrangement might be the norm in archaea . Also, it was suggested that archaeal DnaK sequences are not obviously differentiated from bacterial counterparts and that the archaeal dnaK genes originated by lateral gene transfer from bacteria (Gribaldo et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Evolution of a Protein-Folding Machine: Genomic and Evolutionary Analyses Reveal Three Lineages of the Archaeal hsp70(dnaK) Gene

et al. 2006

Self Cite

View full text Add to dashboard Cite

Abstract. The stress chaperone protein Hsp70 (DnaK) (abbreviated DnaK) and its co-chaperones Hsp40(DnaJ) (or DnaJ) and GrpE are universal in bacteria and eukaryotes but occur only in some archaea clustered in the order 5¢-grpE-dnaK-dnaJ-3¢ in a locus termed Locus I. Three structural varieties of Locus I, termed Types I, II, and III, were identified, respectively, in Methanosarcinales, in Thermoplasmatales and Methanothermobacter thermoautotrophicus, and in Halobacteriales. These Locus I types corresponded to three groups identified by phylogenetic trees of archaeal DnaK proteins including the same archaeal subdivisions. These archaeal DnaK groups were not significantly interrelated, clustering instead with DnaKs from three bacterial lineages, Methanosarcinales with Firmicutes, Thermoplasmatales and M. thermoautotrophicus with Thermotoga, and Halobacteriales with Actinobacteria, suggesting that the three archaeal types of Locus I were acquired by independent events of lateral gene transfer. These associations, however, lacked strong bootstrap support and were sensitive to dataset choice and treereconstruction method. Structural features of dnaK loci in bacteria revealed that Methanosarcinales and Firmicutes shared a similar structure, also common to most other bacterial groups. Structural differences were observed instead in Thermotoga compared to Thermoplasmatales and M. thermoautotrophicus, and in Actinobacteria compared to Halobacteriales. It was also found that the association between the DnaK sequences from Halobacteriales and Actinobacteria likely reflects common biases in their amino acid compositions. Although the loci structural features and the DnaK trees suggested the possibility of lateral gene transfer between Firmicutes and Methanosarcinales, the similarity between the archaeal and the ancestral bacterial loci favors the more parsimonious hypothesis that all archaeal sequences originated from a unique prokaryotic ancestor.

show abstract

“…The deduced amino acid sequences of rat and human mtGrpE exhibit 88.9% positional identity, while positional identities with other GrpE family members are: 41.8°/,, for mitochondrial Droelp from D. melanogaster (GenBank accession number U34903), 33.3% for C. elegans GrpE (EMBL accession number Z46996), 25.9% for mitochondrial Ygelp from S. cerevisiae [7], 20.8% for E.eoli GrpE [20] and 19.1% for archaebacterial M. mazei GrpE [21]. Comparison of the deduced amino acid sequences of 19 GrpE family members reveals that five residues are strictly conserved, four of which reside in the C-terminal third of GrpE (Fig.…”

Section: Members Of the Grpe Family Exhibit A Relatively Low Degree Omentioning

confidence: 99%

Isolation and characterisation of a cDNA encoding rat mitochondrial GrpE, a stress‐inducible nucleotide‐exchange factor of ubiquitous appearance in mammalian organs

1996

View full text Add to dashboard Cite

In contrast to the E. coli chaperones DnaK, GroEL and GroES, cDNAs encoding mitochondrial homologues of DnaJ and GrpE from higher eukaryotes have yet to be reported. Based on peptide sequences, we have isolated a cDNA encoding a 217 residue nuclear encoded precursor of rat mitochondrial GrpE (mt-GrpE) including a typical mitochondrial presequence of 27 residues. Western blotting revealed that the 21 kDa GrpE homologue is present exclusively in the mitochondrial fraction where it comprises only ~ 0.03% of the total soluble protein, while Northern blotting showed that the mt-GrpE transcript is present in most if not all organs. By contrast to other mitochondrial chaperones, the levels of mt-GrpE and its transcript in cultured cells are only marginally increased in response to the proline analog L-azetidine 2-carboxylic acid but not by heat shock. Furthermore, members of the GrpE family exhibit a much lower degree of sequence identity than do the well studied members of the Hsp70, Hsp60 and Hspl0 families.

show abstract

Identification of a grpE Heat-shock Gene Homolog in the Archeon Methanosarcina mazie

Cited by 38 publications

References 0 publications

Phylogenetic Analysis of Mycoplasmas Based on Hsp70 Sequences: Cloning of the dnaK (hsp70) Gene Region of Mycoplasma capricolum

Phylogenetic Analysis of Mycoplasmas Based on Hsp70 Sequences: Cloning of the dnaK (hsp70) Gene Region of Mycoplasma capricolum

Evolution of a Protein-Folding Machine: Genomic and Evolutionary Analyses Reveal Three Lineages of the Archaeal hsp70(dnaK) Gene

Isolation and characterisation of a cDNA encoding rat mitochondrial GrpE, a stress‐inducible nucleotide‐exchange factor of ubiquitous appearance in mammalian organs

Contact Info

Product

Resources

About