Identification of a hybrid atypical enteropathogenic and enteroaggregative Escherichia coli (aEPEC/EAEC) clone of serotype O3:H2 associated with a diarrheal outbreak in Brazil

Lira, Daiany R P de; Cavalcanti, Adriene Maria Ferreira; Pinheiro, Sandra Regina Schicariol; Orsi, Henrique; Santos, Luis Fernando dos; Hernandes, Rodrigo T.

doi:10.1007/s42770-021-00580-6

Cited by 9 publications

(10 citation statements)

References 43 publications

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“…In this study, 32 (2.6%) DEC isolates were hybrid DEC pathotypes. EAEC/EPEC hybrids, similar to an EAEC/ atypical-EPEC hybrid with the eae and aagR gene reported from Brazil in 2021 47 were identified in 10 specimens (4 from cases and 6 from controls, NS). We additionally detected EAEC/ETEC in 16 specimens (5 cases and 7 controls, NS), ETEC/EPEC in 2 case specimens and EAEC/ EIEC in a control specimen.…”

Section: Discussionsupporting

confidence: 63%

“…A hybrid pathotype contains virulence or other loci that would lead to it being classified as more than one DEC pathotype, sometimes conferring greater virulence. Hybrids have been associated with outbreaks 45-47 , however outbreak investigations typically prompt more comprehensive isolate characterization and would therefore be more likely to detect hybrids. In this study, 32 (2.6%) DEC isolates were hybrid DEC pathotypes.…”

Section: Discussionmentioning

confidence: 99%

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Epidemiology and risk factors for diarrhoeagenic Escherichia coli carriage among children in northern Ibadan, Nigeria

Akinlabi

Nwoko

Dada

et al. 2022

Preprint

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Diarrhea is a leading cause of childhood morbidity in Africa. Outside of multi-country sentinels, of which there are none in Nigeria, few studies focus on bacterial etiology. We performed a case-control study among children under five years of age. Stool specimens were collected from 120 children with, and 357 without, diarrhea attending primary health clinics on the northern outskirts of Ibadan between November 2015 and August 2019. Up to ten E. coli isolates were obtained per specimen and at least three were whole genome-sequenced using Illumina technology. Genomes were assembled using SPAdes, quality evaluated using QUAST, and Virulencefinder was used to identify virulence genes. The microbiological quality of water from 14 wells within the study area was assessed using total and coliform counts. Diarrhoeagenic Escherichia coli (DEC) were isolated from 79 (65.8%) of cases and 217 (60.8%) control children. All DEC pathotypes except Shiga toxin-producing E. coli, a number of hybrid DEC pathotypes, Salmonella and Yersina spp. were detected but no pathogen showed association with disease (p>0.05). Enterotoxigenic E. coli were more commonly recovered from younger controls but exclusively detected in cases aged over nine months. Temporally-linked, highly similar enteroaggregative E. coli were isolated from children in different households in eight instances. No well water sample drawn in the study qualified as potable. Children in northern Ibadan are commonly colonized with DEC. Access to water and sanitation, and vaccines targeting the most abundant pathogens may be critical for protecting children from the less overt consequences of enteric pathogen carriage.

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Section: Discussionsupporting

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Epidemiology and risk factors for diarrhoeagenic Escherichia coli carriage among children in northern Ibadan, Nigeria

Akinlabi

Nwoko

Dada

et al. 2022

Preprint

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“…Isolates of E. coli serotype O3:H2, molecularly classified as EAEC, have been frequently isolated from stool samples from patients with diarrhea, as well as from asymptomatic individuals in studies carried out in Brazil [ 12 , 66 , 67 , 68 , 69 ]. In the present study, the hybrid UPEC/EAEC-34 of serotype O3:H2 was isolated from a UTI, thus suggesting that some bacteria of this serotype may act as a heteropathogen.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Uropathogenic Escherichia coli Reveals Hybrid Isolates of Uropathogenic and Diarrheagenic (UPEC/DEC) E. coli

et al. 2022

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(1) Background: Pathogenic Escherichia coli are divided into two groups: diarrheagenic (DEC) and extraintestinal pathogenic (ExPEC) E. coli. ExPEC causing urinary tract infections (UTIs) are termed uropathogenic E. coli (UPEC) and are the most common cause of UTIs worldwide. (2) Methods: Here, we characterized 112 UPEC in terms of phylogroup, serotype, the presence of virulence factor-encoding genes, and antimicrobial resistance. (3) Results: The majority of the isolates were assigned into the phylogroup B2 (41.07%), and the serogroups O6 (12.5%) and O25 (8.9%) were the most frequent. Five hybrid UPEC (4.5%), with markers from two DEC pathotypes, i.e., atypical enteropathogenic (aEPEC) and enteroaggregative (EAEC) E. coli, were identified, and designated UPEC/aEPEC (one isolate) and UPEC/EAEC (four isolates), respectively. Three UPEC/EAEC harbored genes from the pap operon, and the UPEC/aEPEC carried ibeA. The highest resistance rates were observed for ampicillin (46.4%) and trimethoprim/sulfamethoxazole (34.8%), while 99.1% of the isolates were susceptible to nitrofurantoin and/or fosfomycin. Moreover, 9.8% of the isolates were identified as Extended Spectrum β-Lactamase producers, including one hybrid UPEC/EAEC. (4) Conclusion: Our data reinforce that hybrid UPEC/DEC are circulating in the city of Botucatu, Brazil, as uropathogens. However, how and whether these combinations of genes influence their pathogenicity is a question that remains to be elucidated.

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“…Since lysates prepared from an induced lysogen would yield both P1(S) and P1(S’) phages, the factor(s) required for P1(S’) adsorption might be present in E. coli O111:B4, which is consistent with the high P1(S’) neutralising effect of E. coli O111:B4 LPS. Serotyping analysis showed that E. coli O111 is associated with strains of enterotoxigenic E. coli (ETEC) (Campos et al, 1994) while E. coli O3 is associated with clinical isolates of enteroaggregative (EAEC) E. coli (Jenkins et al, 2006; Kai et al, 2010; de Lira et al, 2021). Hence, P1(S’) could be a valuable vector for the delivery of the cas9 antimicrobial phagemid into clinical isolates of E. coli O111 and E. coli O3.…”

Section: Discussionmentioning

confidence: 99%

The host range and the role of O-antigen in P1 transduction with its alternative S’ tail fibre

Huan

Fa-arun

Wang

2022

Preprint

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Enterobacteria phage P1 expresses two types of tail fibre, S and S′. Despite the wide usage of phage P1 for transduction, the host range and the receptor for its alternative S prime tail fibre was never determined. Here, a ΔS-cin Δpac E. coli P1 lysogenic strain was generated to allow packaging of phagemid DNA into P1 phage having either S or S′ tail fibre. P1(S′) could transduce phagemid DNA into Shigella flexneri 2a 2457O, Shigella flexneri 5a M90T and Escherichia coli O3 efficiently. Mutational analysis of the O-antigen assembly genes and LPS inhibition assays indicated that P1(S′) transduction requires at least one O-antigen unit. E. coli O111:B4 LPS produced a high neutralising effect against P1(S′) transduction, indicating that this E. coli strain could be a host for P1(S′). Mutations in the O-antigen modification genes of S. flexneri 2a 2457O and S. flexneri 5a M90T did not cause significant changes to P1(S′) transduction efficiency. A higher transduction efficiency of P1(S′) improved the delivery of a cas9 antimicrobial phagemid into both S. flexneri 2457O and M90T. These findings provide novel insights into P1 tropism-switching, by identifying the host range of P1(S′) and demonstrating its potential for delivering a sequence-specific Cas9 antimicrobial into clinically relevant S. flexneri.

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Identification of a hybrid atypical enteropathogenic and enteroaggregative Escherichia coli (aEPEC/EAEC) clone of serotype O3:H2 associated with a diarrheal outbreak in Brazil

Cited by 9 publications

References 43 publications

Epidemiology and risk factors for diarrhoeagenic Escherichia coli carriage among children in northern Ibadan, Nigeria

Epidemiology and risk factors for diarrhoeagenic Escherichia coli carriage among children in northern Ibadan, Nigeria

Characterization of Uropathogenic Escherichia coli Reveals Hybrid Isolates of Uropathogenic and Diarrheagenic (UPEC/DEC) E. coli

The host range and the role of O-antigen in P1 transduction with its alternative S’ tail fibre

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