Identification of a Hypochlorite-specific Transcription Factor from Escherichia coli

Gebendorfer, Katharina M.; Drazic, Adrian; Yan, Le; Gundlach, Jasmin; Bepperling, Alexander; Kastenmüller, Andreas; Ganzinger, Kristina A.; Braun, Nathalie; Franzmann, Titus M.; Winter, Jeannette

doi:10.1074/jbc.m111.287219

Cited by 69 publications

(146 citation statements)

References 40 publications

(48 reference statements)

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“…Additionally, the killing mechanism of many antibiotics ultimately involves the generation of ROS (reviewed by Dwyer et al, 2009). In E. coli, cellular defenses against ROS include increased expression of enzymes involved in cysteine and methionine biosynthesis (Gebendorfer et al, 2012), and it is tempting to speculate that the increase in the relative abundance of genes involved in cysteine and methionine biosynthesis in MIT9313 cells responding to co-culture may be related to a similar defense mechanism.…”

Section: Resultsmentioning

confidence: 99%

Transcriptional response of Prochlorococcus to co-culture with a marine Alteromonas: differences between strains and the involvement of putative infochemicals

Aharonovich

Sher

2016

The ISME Journal

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Interactions between marine microorganisms may determine the dynamics of microbial communities. Here, we show that two strains of the globally abundant marine cyanobacterium Prochlorococcus, MED4 and MIT9313, which belong to two different ecotypes, differ markedly in their response to coculture with a marine heterotrophic bacterium, Alteromonas macleodii strain HOT1A3. HOT1A3 enhanced the growth of MIT9313 at low cell densities, yet inhibited it at a higher concentration, whereas it had no effect on MED4 growth. The early transcriptomic responses of Prochlorococcus cells after 20 h in co-culture showed no evidence of nutrient starvation, whereas the expression of genes involved in photosynthesis, protein synthesis and stress responses typically decreased in MED4 and increased in MIT313. Differential expression of genes involved in outer membrane modification, efflux transporters and, in MIT9313, lanthipeptides (prochlorosins) suggests that Prochlorococcus mount a specific response to the presence of the heterotroph in the cultures. Intriguingly, many of the differentially-expressed genes encoded short proteins, including two new families of co-culture responsive genes: CCRG-1, which is found across the Prochlorococcus lineage and CCRG-2, which contains a sequence motif involved in the export of prochlorosins and other bacteriocin-like peptides, and are indeed released from the cells into the media.

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Section: Resultsmentioning

confidence: 99%

Transcriptional response of Prochlorococcus to co-culture with a marine Alteromonas: differences between strains and the involvement of putative infochemicals

Aharonovich

Sher

2016

The ISME Journal

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“…However, it dissociates into dimers and tetramers upon incubation with DNA in vitro, thus forming the DNA-binding species (9). Of note, given the reduced state of the protein, DNA binding is only weak (9,10). This DNA-induced dissociation is independent of the DNA used and occurs with nonspecific DNA and target DNA alike (9).…”

mentioning

confidence: 99%

“…The reduced HypT dodecamer is inactive. However, it dissociates into dimers and tetramers upon incubation with DNA in vitro, thus forming the DNA-binding species (9). Of note, given the reduced state of the protein, DNA binding is only weak (9,10).…”

mentioning

confidence: 99%

“…LysR-type transcriptional regulators are ubiquitous regulators that typically form dimers and tetramers (e.g. OxyR (13), CysB (14), and NAC (15)) but can also form large oligomers such as octamers (CrgA (16)) and dodecamers (HypT (9)). The reduced HypT dodecamer is inactive.…”

mentioning

confidence: 99%

“…HypT is a LysR-type transcriptional regulator that specifically protects Escherichia coli cells from HOCl damage (9,12). LysR-type transcriptional regulators are ubiquitous regulators that typically form dimers and tetramers (e.g.…”

mentioning

confidence: 99%

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Tetramers Are the Activation-competent Species of the HOCl-specific Transcription Factor HypT

Drazic

Gebendorfer

Mak

et al. 2014

Journal of Biological Chemistry

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Background:The transcription factor HypT is a dynamic oligomer and is activated by methionine oxidation. Results: Dissociation of dodecamers into tetramers enhances and accelerates HypT activation by HOCl, proving tetramers to be the activation-competent species. Conclusion: HypT activity is controlled by its oligomeric state and exposure to HOCl. Significance: Regulation of a transcription factor by two levels of control enables fine-tuned activation under stress.

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Roles of methionine and cysteine residues of the Escherichia coli sensor kinase HprS in reactive chlorine species sensing

et al. 2023

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Sensor histidine kinase HprS, an oxidative stress sensor of Escherichia coli, senses reactive oxygen species (ROS) and reactive chlorine species (RCS), and is involved in the induction of oxidatively damaged protein repair periplasmic enzymes. We reinvestigated the roles of six methionine and four cysteine residues of HprS in the response to HClO, an RCS. The results of site‐directed mutagenesis revealed that methionine residues in periplasmic and cytoplasmic regions (Met225) are involved in HprS activation. Interestingly, the Cys165Ser substitution reduced HprS activity, which was recovered by an additional Glu22Cys substitution. Our results demonstrate that the position of the inner membrane cysteine residues influences the extent of HprS activation in HClO sensing.

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Identification of a Hypochlorite-specific Transcription Factor from Escherichia coli

Cited by 69 publications

References 40 publications

Transcriptional response of Prochlorococcus to co-culture with a marine Alteromonas: differences between strains and the involvement of putative infochemicals

Transcriptional response of Prochlorococcus to co-culture with a marine Alteromonas: differences between strains and the involvement of putative infochemicals

Tetramers Are the Activation-competent Species of the HOCl-specific Transcription Factor HypT

Roles of methionine and cysteine residues of the Escherichia coli sensor kinase HprS in reactive chlorine species sensing

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