Identification of a major envelope protein in Chlamydia spp

Abstract

A major cell envelope protein of Chlamydia psittaci with a molecular weight of approximately 43,000 was identified and partially characterized. It was present at all stages of the C. psittaci developmental cycle. A major protein with a similar molecular weight was also observed in two Chlamydia trachomatis strains.

Turkeys are protected from infection withChlamydia psittaciby plasmid DNA vaccination against the major outer membrane protein

“…The only protective chlamydial antigen which has been unambiguously identified is the major outer membrane protein (MOMP). This protein, identified independently by two groups in the USA [17,18] and one in the UK [19], represents the majority of the surface exposed proteins of the species C. psittaci. It is a protein of around 40 kD characterized by four variable regions (VS I to IV) and five intervening constant regions of conserved structure and function.…”

Processing of McCoy cell cultures infected with Chlamydia trachomatis: sequential isolation of chlamydial elementary bodies and lipopolysaccharide

“…have obvious advantages, but even the separation of Chlamydia after cultivation in tissue culture cells has proven quite laborious. Chlamydia contain two major antigens: the species-specific major outer membrane protein [2][3][4][5], which has a molecular mass varying between 38 and 43 kDa, and the genus-specific antigen. The molecular mass of the major outer membrane protein of C. trachomatis serotype L2, used in this study, is 39.5 kDa.…”

“…In some studies NP-40, a non-ionic detergent, has been used to enhance the liberation [9]. Various gradient centrifugations in renografin [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] or sucrose [11,12] have been used for their purification. In our hands these centrifugations resulted in poor separation of chlamydial particles as judged from the protein pattern in sodium-dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as well as from the numbers of inclusion-forming units (IFU) in the fractions.…”

Analysis of proteins inChlamydia trachomatisL2 outer membrane complex, COMC

“…The major outer membrane protein (MOMP) is the most dominant protein in this outer membrane complex (COMC) (Caldwell et al, 1981). The size of MOMP is c. 43 kDa with a small variation in size between species and serotypes (Hatch et al, 1981). Additional proteins of 62/59 and 12 kDa were also observed by Hatch et al (1981) in COMC.…”