Identification of a microRNA regulator for axon guidance in the olfactory bulb of adult mice

Sun, Tingting; Li, Shanshan; Yang, Jing; Yin, Yifei; Shao, Ling

doi:10.1016/j.gene.2014.06.063

Cited by 11 publications

(13 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…a). We had found preliminary evidence that the level of sema3A is controlled by miR‐30c . To confirm this relationship, miR‐30c‐KD, which captures miR‐30c by binding to miR‐30c mature sequences, was added in a luciferase assay.…”

Section: Resultsmentioning

confidence: 99%

“…Sema3A, a protein involved in repulsive axon guidance, plays roles in neuronal regeneration and polarity . Having confirmed that miR‐30c negatively regulates the expression of sema3A in human embryonic kidney 293T (HEK293T) cells , and taking these findings together, we set out to determine whether miR‐30c and sema3A play roles in neuronal proliferation and differentiation.…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

miR‐30c and semaphorin 3A determine adult neurogenesis by regulating proliferation and differentiation of stem cells in the subventricular zones of mouse

Sun

Shao

2016

Cell Proliferation

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

miR‐30c and semaphorin 3A determine adult neurogenesis by regulating proliferation and differentiation of stem cells in the subventricular zones of mouse

Sun

Shao

2016

Cell Proliferation

Self Cite

View full text Add to dashboard Cite

show abstract

“…From the microarray results, a total of 104 miRNAs were found to be differentially expressed in NSCs from embryos of diabetic pregnancy when compared to the control. Among the differentially expressed miRNAs, members of miRNA-30 family (miRNA 30a, b, c, d, and e) were chosen for further studies as they have been found to be: (a) upregulated significantly (fold change ranging from 1.15–1.52) in NSCs from embryos of diabetic pregnancy (Supplementary Table 4 ); (b) involved in neurodevelopmental disorders (Mellios and Sur, 2012 ; Hancock et al, 2014 ; Sun et al, 2014 ; Han et al, 2015 ). Further, quantitative RT-PCR analysis was performed to validate the expression levels of miRNA-30 family (miRNA-30 b, c, d, and e) in NSCs from embryos of diabetic and control pregnancy.…”

Section: Resultsmentioning

confidence: 99%

“…The results obtained were compared in order to identify miRNA signatures implicating maternal diabetes-induced neural tube anomalies. Among the differentially expressed miRNAs in NSCs from diabetic pregnancy, the miRNA-30 family has been proposed to play critical role in maternal diabetes-induced neural tube anomalies as it has been shown to be involved in neurodevelopmental disorders (Mellios and Sur, 2012 ; Hancock et al, 2014 ; Sun et al, 2014 ; Han et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Maternal Diabetes Alters Expression of MicroRNAs that Regulate Genes Critical for Neural Tube Development

Ramya

Sudhaharan

Bay

et al. 2017

Front. Mol. Neurosci.

View full text Add to dashboard Cite

Maternal diabetes is known to cause neural tube defects (NTDs) in embryos and neuropsychological deficits in infants. Several metabolic pathways and a plethora of genes have been identified to be deregulated in developing brain of embryos by maternal diabetes, although the exact mechanism remains unknown. Recently, miRNAs have been shown to regulate genes involved in brain development and maturation. Therefore, we hypothesized that maternal diabetes alters the expression of miRNAs that regulate genes involved in biological pathways critical for neural tube development and closure during embryogenesis. To address this, high throughput miRNA expression profiling in neural stem cells (NSCs) isolated from the forebrain of embryos from normal or streptozotocin-induced diabetic pregnancy was carried out. It is known that maternal diabetes results in fetal hypoglycemia/hyperglycemia or hypoxia. Hence, NSCs from embryos of control pregnant mice were exposed to low or high glucose or hypoxia in vitro. miRNA pathway analysis revealed distinct deregulation of several biological pathways, including axon guidance pathway, which are critical for brain development in NSCs exposed to different treatments. Among the differentially expressed miRNAs, the miRNA-30 family members which are predicted to target genes involved in brain development was upregulated in NSCs from embryos of diabetic pregnancy when compared to control. miRNA-30b was found to be upregulated while its target gene Sirtuin 1 (Sirt1), as revealed by luciferase assay, was down regulated in NSCs from embryos of diabetic pregnancy. Further, overexpression of miRNA-30b in NSCs, resulted in decreased expression of Sirt1 protein, and altered the neuron/glia ratio. On the other hand, siRNA mediated knockdown of Sirt1 in NSCs promoted astrogenesis, indicating that miRNA-30b alters lineage specification via Sirt1. Overall, these results suggest that maternal diabetes alters the genes involved in neural tube formation via regulating miRNAs.

show abstract

“…Of these microRNAs identified by microarray (data was not published), we found that miR-142-5p expression was strongly induced by PHEV over 8-fold at 5 dpi, and that altering miR-142-5p activity resulted in disordered neuronal morphogenesis in primary cortical neurons. Given that, some microRNAs have been reported to play crucial roles in CNS development, including axon guidance, dendritic morphogenesis, and synaptic plasticity (Wibrand et al, 2012;Sun et al, 2014;Liu et al, 2015;Ristori et al, 2015). Therefore, we attempted to explore the mechanism of nervous system dysfunction caused by PHEV from the perspective of the host's differentially expressed microRNA miR-142-5p.…”

Section: Discussionmentioning

confidence: 99%

miR-142-5p Disrupts Neuronal Morphogenesis Underlying Porcine Hemagglutinating Encephalomyelitis Virus Infection by Targeting Ulk1

Lan

Zhao

et al. 2017

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Porcine hemagglutinating encephalomyelitis virus (PHEV) invades the central nervous system (CNS) and causes neurodegenerative disease in suckling piglets, but the understanding of its neuropathogenicity for neurological dysfunction remains limited. Here, we report that miR-142-5p is localized to neurons and negatively regulates neuronal morphogenesis in porcine hemagglutinating encephalomyelitis (PHE). This phenotype was mediated by miR-142-5p inhibition of an mRNA encoding unc-51-like-kinase1 (Ulk1), which controls axon outgrowth and dendrite formation. Modulating miR-142-5p activity by microRNA mimics or inhibitors induced neurodegeneration, including stunted axon elongation, unstable dendritic spine formation, and irregular swelling and disconnection in neurites. Relieving Ulk1 mRNA repression in primary cortical neurons by miR-142-5p antagomirs or replication-deficient adenoviruses encoding Ulk1 (Ad5-Ulk1), which improved rescue of nerve injury, restricted viral replication, and increased survival rate in mice underlying PHEV infection. In contrast, disrupting Ulk1 in RNAi-expressing neurons mostly led to significantly shortened axon elongation and/or an abnormally large number of branched dendrites. Taken together, we demonstrated that the abnormal neuronal morphogenesis underlying PHEV infection was mainly caused by functional mRNA repression of the miR-142-5p target Ulk1. Our data revealed that PHEV adapted to use spatiotemporal control of host microRNAs to invade CNS, and provided new insights into the virus-associated neurological dysfunction microenvironment.

show abstract

Identification of a microRNA regulator for axon guidance in the olfactory bulb of adult mice

Cited by 11 publications

References 40 publications

miR‐30c and semaphorin 3A determine adult neurogenesis by regulating proliferation and differentiation of stem cells in the subventricular zones of mouse

miR‐30c and semaphorin 3A determine adult neurogenesis by regulating proliferation and differentiation of stem cells in the subventricular zones of mouse

Maternal Diabetes Alters Expression of MicroRNAs that Regulate Genes Critical for Neural Tube Development

miR-142-5p Disrupts Neuronal Morphogenesis Underlying Porcine Hemagglutinating Encephalomyelitis Virus Infection by Targeting Ulk1

Contact Info

Product

Resources

About