Identification of a Movement Protein of Rice Yellow Stunt Rhabdovirus

Huang, Yanwei; Geng, Yun-Feng; Ying, Xiaobao; Chen, Xiaoying; Fang, Rongxiang

doi:10.1128/jvi.79.4.2108-2114.2005

Cited by 70 publications

(56 citation statements)

References 38 publications

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“…The P:P3 interaction is characterized by the re-localization of some P3 from the cell periphery to the nucleus. GST pull-down assays showed that RYSV P3 specifically binds to cognate N protein, indicating its involvement in cell-to-cell movement (Huang et al, 2005). On the other hand, the putative movement protein of PYDV was shown to interact with cognate M and G proteins (Bandyopadhyay et al, 2010), whereas SYNV sc4 interacts with the G protein (Min et al, 2010).…”

Section: Discussionmentioning

confidence: 96%

Complete genome sequence and integrated protein localization and interaction map for alfalfa dwarf virus, which combines properties of both cytoplasmic and nuclear plant rhabdoviruses

et al. 2015

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We have determined the full-length 14,491-nucleotide genome sequence of a new plant rhabdovirus, alfalfa dwarf virus (ADV). Seven open reading frames (ORFs) were identified in the antigenomic orientation of the negative-sense, single-stranded viral RNA, in the order 3'-N-P-P3-M-G-P6-L-5'. The ORFs are separated by conserved intergenic regions and the genome coding region is flanked by complementary 3' leader and 5' trailer sequences. Phylogenetic analysis of the nucleoprotein amino acid sequence indicated that this alfalfa-infecting rhabdovirus is related to viruses in the genus Cytorhabdovirus. When transiently expressed as GFP fusions in Nicotiana benthamiana leaves, most ADV proteins accumulated in the cell periphery, but unexpectedly P protein was localized exclusively in the nucleus. ADV P protein was shown to have a homotypic, and heterotypic nuclear interactions with N, P3 and M proteins by bimolecular fluorescence complementation. ADV appears unique in that it combines properties of both cytoplasmic and nuclear plant rhabdoviruses.

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Section: Discussionmentioning

confidence: 96%

Complete genome sequence and integrated protein localization and interaction map for alfalfa dwarf virus, which combines properties of both cytoplasmic and nuclear plant rhabdoviruses

et al. 2015

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“…The extra gene that we have named Y is in a similar genomic context to the sc4 gene of SYNV, and P3 of RYSV, both of which have been implicated in cell-to-cell movement in these viruses (Huang et al, 2005; Melcher, 2000). The subcellular localization pattern of PYDV-Y is on the periphery of the cells and in this respect is highly similar to that of sc4 (Goodin et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

An integrated protein localization and interaction map for Potato yellow dwarf virus, type species of the genus Nucleorhabdovirus

et al. 2010

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The genome of Potato yellow dwarf virus (PYDV; Nucleorhabdovirus type species), was determined to be 12,875 nucleotides (nt). The antigenome is organized into seven open reading frames (ORFs) ordered 3′-N-X-P-Y-M-G-L-5′, which likely encode the nucleocapsid, phospho, movement, matrix, glyco and RNA-dependent RNA polymerase proteins, respectively, except for X, which is of unknown function. The ORFs are flanked by a 3′ leader RNA of 149 nt and a 5′ trailer RNA of 97 nt, and are separated by conserved intergenic junctions. Phylogenetic analyses indicated that PYDV is closely related to other leafhopper-transmitted rhabdoviruses. Functional protein assays were used to determine the subcellular localization of PYDV proteins. Surprisingly, the M protein was able to induce the intranuclear accumulation of the inner nuclear membrane in the absence of any other viral protein. Finally, bimolecular fluorescence complementation was used to generate the most comprehensive protein interaction map for a plant-adapted rhabdovirus to date.

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“…For example, MPs of Rice dwarf virus and Rice yellow stunt virus were used to complement PVX mutant movement in N. benthamiana plants (11,18). Even more interesting, Flock House virus, an insect virus, can spread in N. benthamiana plants expressing MP of TMV or Red clover necrotic mosaic virus (7).…”

Section: Discussionmentioning

confidence: 99%

“…Construct pBI-P25, expressing the PVX p25 protein, was provided by R. X. Fang (Institute of Microbiology, Chinese Academy of Sciences) (11). Six ORFs of RSV (i.e., NS2, NSvc2, NS3, NCP, NSvc4, and SP) were amplified individually from total RNA extracted from RSV-infected rice tissues collected from a rice field in the Jiangsu province using Pfu DNA polymerase and specific primer pairs (NS2-F/NS2-R for NS2, NSvc2-F/NSvc2-R for NSvc2, NS3-F/NS3-R for NS3, NCP-F/NCP-R for NCP, NSvc4-F/NSvc4-R for NSvc4, and SP-F/SP-R for SP) ( Table 1).…”

Section: Constructs and Procedures For Complementation Experimentsmentioning

confidence: 99%

Identification of a Movement Protein of the Tenuivirus Rice Stripe Virus

Xiong

Zhou

et al. 2008

J Virol

141

138

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Rice stripe virus (RSV) is the type member of the genus Tenuivirus. RSV has four single-stranded RNAs and causes severe disease in rice fields in different parts of China. To date, no reports have described how RSV spreads within host plants or the viral and/or host factor(s) required for tenuivirus movement. We investigated functions of six RSV-encoded proteins using trans-complementation experiments and biolistic bombardment. We demonstrate that NSvc4, encoded by RSV RNA4, supports the intercellular trafficking of a movementdeficient Potato virus X in Nicotiana benthamiana leaves. We also determined that upon biolistic bombardment or agroinfiltration, NSvc4:enhanced green fluorescent protein (eGFP) fusion proteins localize predominantly near or within the walls of onion and tobacco epidermal cells. In addition, the NSvc4:eGFP fusion protein can move from initially bombarded cells to neighboring cells in Nicotiana benthamiana leaves. Immunocytochemistry using tissue sections from RSV-infected rice leaves and an RSV NSvc4-specific antibody showed that the NSvc4 protein accumulated in walls of RSV-infected leaf cells. Gel retardation assays revealed that the NSvc4 protein interacts with single-stranded RNA in vitro, a common feature of many reported plant viral movement proteins (MPs). RSV NSvc4 failed to interact with the RSV nucleocapsid protein using yeast two-hybrid assays. Taken together, our data indicate that RSV NSvc4 is likely an MP of the virus. This is the first report describing a tenuivirus MP. Rice stripe virus (RSV) is the type member of the genusTenuivirus (Association of Applied Biologists Descriptions of Plant Viruses website [http://www.dpvweb.net/dpv/showdpv .php?dpvnoϭ375]). RSV causes severe diseases in rice fields, especially in China, and is known to be transmitted by the small brown plant hopper (Laodelphax striatellus) in a persistent, circulative-propagative manner (8,46). RSV is an RNA virus with four segmented single-stranded genomes, contains seven open reading frames (ORFs), and uses a negative and ambisense coding strategy for replication and infection in plants ( Fig. 1) (32). RNA1 is negative sense and encodes a putative protein with a molecular mass of approximately 337 kDa; this protein is reported to be part of the RNA-dependent RNA polymerase and is associated with the RSV filamentous ribonucleoprotein (42). RNAs 2 to 4 are ambisense, and each RNA contains two ORFs, one in the 5Ј half of the viral RNA and the other in the 5Ј half of the viral cRNA. RNA2 encodes NS2 (about 22.8 kDa, with an unknown function) from the viral RNA and NSvc2 (94 kDa, a putative membrane glycoprotein) from the viral cRNA. RNA3 encodes a 23-kDa protein that functions as a suppressor of gene silencing (R. Xiong et al., unpublished data) and the nucleocapsid protein (NCP) (35 kDa). The nonstructural disease-specific protein (SP) (21.5 kDa) and NSvc4 (32.5 kDa, with unknown function) are encoded by RNA4 (14,15,31,38,47,48).Numerous studies have indicated that plant viruses encode specific proteins known...

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Identification of a Movement Protein of Rice Yellow Stunt Rhabdovirus

Abstract: Rice yellow stunt virus (RYSV) is a member of the plantinfecting Nucleorhabdovirus genus of the Rhabdoviridae family (35). RYSV has a nonsegmented, negative-sense single-stranded RNA genome which contains a 5Ј trailer, a 3Ј leader, and seven open reading frames (ORFs) in the order

Cited by 70 publications

References 38 publications

Complete genome sequence and integrated protein localization and interaction map for alfalfa dwarf virus, which combines properties of both cytoplasmic and nuclear plant rhabdoviruses

Complete genome sequence and integrated protein localization and interaction map for alfalfa dwarf virus, which combines properties of both cytoplasmic and nuclear plant rhabdoviruses

An integrated protein localization and interaction map for Potato yellow dwarf virus, type species of the genus Nucleorhabdovirus

Identification of a Movement Protein of the Tenuivirus Rice Stripe Virus

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