Identification of a necessary element for Toxoplasma gondii SAG1 gene expression

Fluorescent protein and luciferase genes are valuable reporter genes and have been widely used for noninvasive monitoring of gene expression in living tissues and cells. We tested expression of the dual reporter genes in transient transfection of purified Toxoplasma gondii tachyzoites. Two copies of the enhanced yellow fluorescent protein (EYFP) gene were put under the control of 3 representative T. gondii promoters (GRA1, SAG1, and DHFR). Fluorescence from each EYFP reporter was significantly higher than that from a green fluorescent protein (GFP) reporter. The GRA1-EYFP reporter gave the highest fluorescence. Although both fluorescence and luciferase were expressed in the dual reporter system, the luciferase reporter was more efficient than either the EYFP or GFP reporters, and it required fewer parasites to be successfully used.

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Optimized expression of dual reporter genes in transient transfection of purifiedToxoplasma gondiiusing different promoters

Hao

Yang

et al. 2012

Can. J. Microbiol.

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Identification of a necessary element for Toxoplasma gondii SAG1 gene expression

Cited by 1 publication

References 22 publications

Optimized expression of dual reporter genes in transient transfection of purifiedToxoplasma gondiiusing different promoters

Optimized expression of dual reporter genes in transient transfection of purifiedToxoplasma gondiiusing different promoters

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