Identification of a New Plasmid-Encoded sec -Dependent Bacteriocin Produced by Listeria innocua 743

Abstract

Listeria innocua 743 produces an inhibitory activity demonstrating broad-spectrum inhibition of Listeria monocytogenes isolates. Gel-electrophoretic analysis of culture supernatants indicated that two inhibitors with different molecular weights were produced by this strain. Insertion of Tn917 into a 2.9 Kb plasmid (pHC743) generated mutants with either an impaired ability or a loss in ability to produce one of the inhibitors. This finding confirms that the phenotype is dependent on the structural and immunity gene only and that export of this bacteriocin is sec dependent. This is the first confirmation of bacteriocin production in a Listeria spp., and it is of interest that this bacteriocin is closely related to the pediocin family of bacteriocins produced by lactic acid bacteria.

The Continuing Story of Class IIa Bacteriocins

“…How these genes are organized in operons has been discussed in a previous review (55). A few class IIa bacteriocins (bacteriocin 31, enterocin P, and listeriocin 431) are secreted by the general sec-dependent export system, and for these the exact number of genes required for bacteriocin production is unknown (35,86,102,174).…”

Genetic characterisation and heterologous expression of leucocin C, a class IIa bacteriocin from Leuconostoc carnosum 4010

“…Peptides in subgroup 3 as well as enterocin SE-K4 and carnobacteriocin B2 in subgroup 4 exceptionally have neither the conserved tryptophan at the C-tails, nor the cysteine residues to form the C-terminal disulfide bridge (Table 2). Marrec et al 2000Metivier et al 1998Aymerich et al 1996Henderson et al 1992Marugg et al 1992Tichaczek et al 1994Kalmokoff et al 2001Birri et al 2010Feng 2009Kawamoto et al 2002Bennik et al 1998Yamazaki et al 2005Bhugaloo-Vial et al 1996Jack et al 1996Vaughan et al 2001Fimland et al 2002bHeng et al 2007 Subgroup 2 Kwaadsteniet et al 2006Quadri et al 1995Eguchi et al 2001Diep et al 2006 The YGNGV/L "pediocin box" is marked (bold), the leucine residue (L), odd, and uncertain amino acids (X) are marked as bold and red. Names of the six newly identified class IIa bacteriocins are in red (Rea et al 2011).…”

“…However, several class IIa bacteriocins, for example, listeriocin 743A (Kalmokoff et al 2001), bacteriocin 31 (Tomita et al 1996) and enterocin P (Cintas et al 1997) have been found to have a sectype leader. These bacteriocins are assumed to be secreted by sec-dependent exporting pathway.…”

Molecular analysis of the bacteriocin-encoding plasmid pDGL1 from Enterococcus durans and genetic characterization of the durancin GL locus

“…Depending on their transportation and processing pathways, several subclass IIa bacteriocins utilize the sec-dependent translocation system, including enterocin P (Herranz & Driessen, 2005), bacteriocin 31 (Tomita et al, 1996), enterocin T8 (De Kwaadsteniet et al, 2006) and listeriocin 743A (Kalmokoff et al, 2001), while many of the subclass IIa bacteriocins, especially those containing a double-glycine leader peptide, are secreted by a membrane-associated dedicated ATP-binding cassette (ABC) transporter that concomitantly cleaves off the leader sequence (Nissen-Meyer et al, 2009). The genetic elements for the double-glycine type bacteriocins usually include a processing gene, which encodes a dedicated ABC transporter that helps to process and transport the bacteriocin, and an exporting gene, which encodes an accessory protein required for bacteriocin externalization (Håvarstein et al, 1995;Nes et al, 1996;Dimov et al, 2005;Birri et al, 2010).…”