Identification of a New Powdery Mildew Resistance Gene pmDHT at or Closely Linked to the Pm5 Locus in the Chinese Wheat Landrace Dahongtou

Abstract

Chinese wheat landrace Dahongtou was resistant to 35 of 38 tested Chinese isolates of Blumeria graminis f. sp. tritici at the seedling stage. Genetic analysis of the F2 populations and their derived F2:3 families of crosses of Dahongtou with the susceptible varieties Mingxian 169 and Huixianhong indicated that the resistance of Dahongtou to B. graminis f. sp. tritici isolate E09 was conferred by a single recessive gene, tentatively designated as pmDHT. The gene was mapped to chromosome arm 7BL and flanked by markers Xwmc526/XBE443877 and Xgwm611/Xwmc511 at genetic distances of 0.8 and 0.3 cM, respectively. The chromosomal position of pmDHT was similar to the multi-allelic Pm5 locus on 7BL. Allelism tests with crosses of Dahongtou with Fuzhuang 30 (Pm5e) and Xiaobaidong (mlxbd) indicated that pmDHT was allelic to both Pm5e and mlxbd. However, pmDHT showed a different pattern of resistance to the 38 B. graminis f. sp. tritici isolates compared with wheat lines with Pm5a, Pm5b, Pm5e, mlxbd, and PmHYM and also differed from PmSGA. Thus, pmDHT was identified most likely as a new allele or at least a closely linked gene of the Pm5 locus. This gene can be transferred into susceptible wheat cultivars/lines and pyramided with other resistance genes through marker-assisted selection to improve powdery mildew resistance.

Mining for New Sources of Resistance to Powdery Mildew in Genetic Resources of Winter Wheat

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Molecular Cytogenetic Identification of a New Wheat-Rye 6R Addition Line and Physical Localization of Its Powdery Mildew Resistance Gene

“…Seedling stage reactions of YT2 to 22 single-pustule-derived isolates were separately assessed with three replicates at the greenhouse as described ( Qie et al, 2019 ). These isolates were collected from different wheat production regions of China and were characterized by their individual identifying host ( Supplementary Table 1 ).…”

The Pm5e Gene Has No Negative Effect on Wheat Agronomic Performance: Evidence From Newly Established Near-Isogenic Lines

“…Pm5e was initially detected in Fuzhuang 30, a cultivar selected from Jinghui 30 that was developed from a cross between two Chinese wheat landraces Liquanheshangtou and Huaxianqisifeng in Shaanxi Province in the 1940s ( Huang et al, 2003 ). Subsequent studies identified several genes allelic to Pm5e in wheat landraces, e.g., Hongquanmang ( PmH ) ( Zhou et al, 2005 ), Hongyoumai ( pmHYM ) ( Fu et al, 2017 ), Baiyouyantiao ( PmBYYT ) ( Xu et al, 2018a ), Shangeda ( PmSGD ) ( Xu et al, 2018b ), Dahongtou ( pmDHT ) ( Qie et al, 2019 ), Youbailan ( pmYBL ) ( Xu et al, 2020 ), Xiaobaidong ( mlxbd ) ( Xue et al, 2009 ), and Duanganmang ( PmDGM ) ( Wu et al, 2021 ). Most of these landraces were ever grown in the Guanzhong Plain, Shaanxi Province, or nearby regions ( Xie et al, 2020 ).…”