Identification of a new variant of Chlamydia trachomatis in Mexico

Escobedo-Guerra, Marcos R.; Katoku-Herrera, Mitzuko; López‐Hurtado, Marcela; Villagrana-Zesati, Jesús Roberto; Haro-Cruz, María de Jesús de; Guerra‐Infante, Fernando M.

doi:10.1016/j.eimc.2018.02.008

Cited by 10 publications

(17 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…cryptic plasmid. PCRs analysis detected the 377 bp deletion in orf1 , a 44 bp duplication in orf3 , and a Single Nucleotide Polymorphism (SNP) in orf4 , all known to be characteristic of nvCT [3] and, with the exception of the duplication in orf3 , were all found in the new variant of the nvCT (nvCT-D) that was identified recently in Mexico [4]. Therefore, the obtained data suggested the presence in our specimen of the nvCT variant that went undetected by using the PCR-Ep kit targeting the plasmid gene pgp3 (orf3) .…”

Section: Casementioning

confidence: 99%

“…genovar E variants on the dendrogram (Figure 2a). In contrast, nvCT-D belonging to genovar D [4] was assigned to another cluster corresponding to the genovar D strains (Figure 2a).…”

Section: Casementioning

confidence: 99%

“…As a result, the nvCT proportion markedly decreased in the counties implementing these diagnostic systems, falling from 56% in 2007 to 6.5% in 2015 [21]. In fact, only isolated cases of Chlamydia genital infection caused by nvCT have been reported worldwide recently [2,4,22,23,26,27]. However, it is clear that the reports of such nvCT-caused cases in Chlamydia patients could be relatively rare because the majority of countries only use NAATs for the detection of wtCT [1,9,17,20,27,28].…”

Section: Introductionmentioning

confidence: 99%

“…However, a recent report described a new C.t. variant in Chlamydia patients, which also contained an identical deletion of 377 bp in the plasmid orf1 [4]. This novel strain was detected in Mexico and showed a distinct polymorphism of the ompA and pmpH genes.…”

Section: Introductionmentioning

confidence: 99%

“…In contrast to the Swedish nvCT strains belonging to genovar E [2,18,21,23,24,26,27,29], the Mexican C.t. strain was identified as genovar D (nvCT-D), and showed the absence of a 44 bp perfect tandem duplication within the plasmid orf3 characteristic of nvCT [4].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

An Asymptomatic Patient with Fatal Infertility Carried a Swedish Strain of Chlamydia trachomatis with Additional Deletion in The Plasmid orf1 that Belonged to A Different MLST Sequence Type

et al. 2019

View full text Add to dashboard Cite

Here, we present the first case of asymptomatic genital Chlamydial infection caused by the new emerging Chlamydia trachomatis (C.t.) ST13 strain genovar E, which has a double deletion of 377 bp and 17 bp in orf1 gene of the cryptic plasmid (ddCT). This case occurred in an infertile patient (case-patient) with a detectable level of Chlamydial antibodies and a spermatozoa deficiency known as azoospermia. Additionally, the ddCT strain showed the presence of a duplication of 44 bp in the plasmid orf3 and SNP in orf4, which were known as the typical characteristics of the Swedish variant of C.t. (nvCT) genovar E. Multilocus sequence typing (MLST) determined a significant difference between ddCT and nvCT in four alleles (oppA, hfiX, gitA and enoA). Both ddCT and nvCT were assigned to different genetic lineages and could be allocated to two different non-overlapping clonal complexes. Furthermore, ddCT demonstrated a considerable difference among 4-5 alleles in comparison with other C.t. strains of genovar E of ST4, ST8, ST12, and ST94, including the founder of a single relevant cluster, wtCT E/SW3 (Swedish genetic lineage). In contrast to other genovar E strains, ddCT had identical alleles with seven out of seven loci found in ST13 strains of genovars D and G, including the founder for this clonal group, D/UW-3/CX, and six out of seven loci found in its derivatives, such as ST6, ST10, and ST95 of genovars G and H. Nevertheless, MSTree V2 showed that ddCT and nvCT could have a common early ancestor, which is a parental C.t. G/9301 strain of ST9. A significant difference between ddCT and nvCT of genovar D (nvCT-D) that was recently found in Mexico was also determined as: (i) ddCT belonged to genovar E but not to genovar D; (ii) ddCT had a 44 bp duplication within the orf3 of the plasmid typical for nvCT; (iii) ddCT possessed an additional 17 bp deletion in the orf1. In conclusion, improved case management should include the clinical physician’s awareness of the need to enhance molecular screening of asymptomatic Chlamydia patients. Such molecular diagnostics might be essential to significantly reducing the global burden of Chlamydial infection on international public health.

show abstract

Section: Casementioning

confidence: 99%

“…genovar E variants on the dendrogram (Figure 2a). In contrast, nvCT-D belonging to genovar D [4] was assigned to another cluster corresponding to the genovar D strains (Figure 2a).…”

Section: Casementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

An Asymptomatic Patient with Fatal Infertility Carried a Swedish Strain of Chlamydia trachomatis with Additional Deletion in The Plasmid orf1 that Belonged to A Different MLST Sequence Type

et al. 2019

View full text Add to dashboard Cite

show abstract

Diversity of Genotyping Chlamydia Trachomatis Serovars in Urogenital Samples from Mexican Patients: A Molecular and Bioinformatic Characterization

et al. 2022

View full text Add to dashboard Cite

Identifying the impact of Chlamydia trachomatis screening and treatment on mother-to child transmission and respiratory neonatal outcomes in Mexico

Arteaga-Troncoso¹,

López‐Hurtado²,

Yescas-Buendía³

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Unfavorable pregnancy outcomes caused by Chlamydia trachomatis (C. trachomatis), such as premature rupture of membranes, preterm birth, and low birth weight, are well known. Mother-to-child transmission can occur at the time of birth and may result in conjunctivitis and pneumonia in the newborn. We therefore evaluated to question whether just one maternal screening for Chlamydia is enough to prevent adverse pregnancy and negative neonatal outcomes. Among the 4,087 first-time gynecological-obstetric consultations granted at the National Institute of Perinatology in 2018, we selected the study population according to a case-cohort design. Antenatal Chlamydia screening and treatment interventions were performed on 628 pregnant women using COBAS® TaqMan CT. Chlamydia DNA was also detected in samples from 157 infants of these mothers. We find that C. trachomatis positivity was detected in 10.5%. The vertical transmission rates were 1.5% for thecohort of mothers with a Chlamydia-positive test and 29.7% for those with a negative test. By evaluating symptomatic neonatal infection, the hazard rate of perinatal pneumonia was 3.6 times higher in Chlamydia-positive babies than in Chlamydia-negative babies. Despite the low rate of mother-to-child transmission in women positive for Chlamydia trachomatisdetected with a nucleic acid amplification test and who received timely treatment, possible maternal reinfectionthat is not detected during pregnancy significantly increases the risk of neonatal infection with consequent perinatal pneumonia.

show abstract

Identification of a new variant of Chlamydia trachomatis in Mexico

Cited by 10 publications

References 26 publications

An Asymptomatic Patient with Fatal Infertility Carried a Swedish Strain of Chlamydia trachomatis with Additional Deletion in The Plasmid orf1 that Belonged to A Different MLST Sequence Type

An Asymptomatic Patient with Fatal Infertility Carried a Swedish Strain of Chlamydia trachomatis with Additional Deletion in The Plasmid orf1 that Belonged to A Different MLST Sequence Type

Diversity of Genotyping Chlamydia Trachomatis Serovars in Urogenital Samples from Mexican Patients: A Molecular and Bioinformatic Characterization

Identifying the impact of Chlamydia trachomatis screening and treatment on mother-to child transmission and respiratory neonatal outcomes in Mexico

Contact Info

Product

Resources

About