Identification of a newly isolated <i>Sphingomonas</i> sp. LZ1 and its application to biosynthesize chiral alcohols

Microbial alcohol dehydrogenases: recent developments and applications in asymmetric synthesis

“…Together with bacteria, yeast and fungi also play a prominent role in biotransformations. In addition to the bacterial strains mentioned above, 37 Wang et al reported a fungal strain, Rhodotorula mucilaginosa NQ1, isolated from soil for the reduction of 3,5-bis(trifluoromethyl)acetophenone ( 4 ) to ( S )-[3,5-bis(trifluoromethyl)-phenyl]ethanol ( 5 ) (Scheme 6). 58 After systematic optimization studies, 80 mmol L −1 of substrate 4 was reduced using 250 g (wet weight) per L of resting cells and 35 g L −1 of glucose as the cosubstrate, which led to product 5 in good isolated yield (82%) and ee (99%).…”

“…LZ1, isolated from a soil sample (Scheme 2). 37 A systematic investigation of reaction parameters such as the effects of pH, temperature, cosubstrate, substrate concentration, and resting cell concentrations on the bioreduction of 4 were examined. The reduced product, (S)-[3,5-bis(trifluoromethyl)-phenyl] ethanol (5), was obtained in good yield (94%) with an excellent ee (99.6%).…”

“…Organic & Biomolecular Chemistry corresponding keto substrate, BOPC (37), via dynamic kinetic reduction. While racemization of BOPC could occur at pH ≥10 and ≥45 °C, these conditions remain unfavourable for keto reductases.…”

Biocatalytic Asymmetric Synthesis of (S)‐1‐[3,5‐bis(trifluoromethyl)phenyl]ethanol by an Immobilized KRED in Batch and Flow Conditions

“…Several microorganisms have been reported in recent years, such as Cyberlindnera saturnus, [25] Sphingomonas sp., [26] Candida tropicalis, [27] Rhodotorula mucilaginosa, [28] Rhodococcus erytropolis, [29] Saccharomyces rhodotorula. [30] Medium engineering and in particular the use of deep eutectic solvents (DESs) was explored in some of these studies [25,27a,29] to overcome the problem of unsatisfactory results with whole cell biocatalysis.…”