Identification of a novel cell-penetrating peptide derived from the capsid protein of chicken anemia virus and its application in gene delivery

Hu, Gaowei; Miao, Yingjie; Luo, Xi; Chu, Wenhui; Fu, Yongqian

doi:10.1007/s00253-020-10988-z

Cited by 9 publications

(5 citation statements)

References 51 publications

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“…Once screening and identification have been completed, every peptide must be examined for its potential cell-penetrating activity, which is a time-consuming and laborious cycle (Manavalan et al, 2018 ). Few papers (Hu et al, 2020 ; Kardani and Bolhassani, 2021 ) published showed that bioinformatic tools were used to predict CPP, however, they did not predict the CPP systematically such as CPP prediction, penetration efficiency, structural, peptide-membrane interaction, physical-chemical properties, and wet-lab experiment validation. We found that peptide P2 has higher penetration efficiency than MT23 but lower than TAT, hPP10 and Dot1l.…”

Section: Discussionmentioning

confidence: 99%

In silico identification and experimental validation of cellular uptake and intracellular labeling by a new cell penetrating peptide derived from CDN1

et al. 2021

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Bioactive therapeutic molecules are generally impermeable to the cell membrane, hindering their utility and efficacy. A group of peptides called cell-penetrating peptides (CPPs) were found to have the capability of transporting different types of cargo molecules across the cell membrane. Here, we identified a short peptide named P2, which has a higher proportion of basic residues than the CDN1 (cyclin-dependent kinase inhibitor 1) protein it is derived from, and we used bioinformatic analysis and experimental validation to confirm the penetration property of peptide P2. We found that peptide P2 can efficiently enter different cell lines in a concentration-dependent manner. The endocytosis pathway, especially receptor-related endocytosis, may be involved in the process of P2 penetration. Our data also showed that peptide P2 is safe in cultured cell lines and red blood cells. Lastly, peptide P2 can efficiently deliver self-labeling protein HaloTag into cells for imaging. Our study illustrates that peptide P2 is a promising imaging agent delivery vehicle for future applications.

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Section: Discussionmentioning

confidence: 99%

In silico identification and experimental validation of cellular uptake and intracellular labeling by a new cell penetrating peptide derived from CDN1

et al. 2021

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“…They discovered its ability to deliver red fluorescent protein (RFP) and apoptosis genes into cells, inducing cell apoptosis. 60 PDX-1, a critical transcription factor controlling pancreatic β-cell development, possesses a protein transduction domain for cellular entry. 61 Recombinant PDX-1 was found to enter cells, improving the blood glucose conditions in diabetic mice.…”

Section: Cell-penetrating Peptides (Cpps)mentioning

confidence: 99%

“…In 2020, Hu and colleagues identified a novel CPP-CVP1-N2 within chicken anemia virus. They discovered its ability to deliver red fluorescent protein (RFP) and apoptosis genes into cells, inducing cell apoptosis . PDX-1, a critical transcription factor controlling pancreatic β-cell development, possesses a protein transduction domain for cellular entry .…”

Section: Supercharged Proteinsmentioning

confidence: 99%

Emerging Landscape of Supercharged Proteins and Peptides for Drug Delivery

Wang,

Geng,

Wang

2024

ACS Pharmacol. Transl. Sci.

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Although groundbreaking biotechnological techniques such as gene editing have significantly progressed, the effective and targeted transport of therapeutic agents into host cells remains a major obstacle to the development of biotherapeutics. Confronting the unique challenge posed by large macromolecules such as proteins, peptides, and nucleic acids adds complexity to this issue. Recent findings reveal that the supercharging of proteins and peptides not only enables control over critical properties, such as temperature resistance and catalytic activity, but also holds promise as a viable strategy for their use in drug delivery. This review provides a concise summary of the attributes of supercharged proteins and peptides, encompassing both their natural occurrence and engineered variants. Furthermore, it sheds light on the present status and future possibilities of supercharged proteins and peptides as carriers for significant biomolecules in the realms of medical research and therapeutic applications.

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“…Thereafter, PepM and PepR from the dengue virus capsid protein were discovered [43]. In recent years, the mitochondrial-penetrating peptide MtCPP-1 [44]; skin-permeable IMT-P8 [45]; bioreducible B-mR9 [46]; Tat-NTS, which can inhibit the transcriptional activity of p53 [47]; VP1, derived from the VP1 protein of chicken anemia virus [48]; Ara-27, from Arabidopsis thaliana [49]; and peptide SOX2-iPep have been reported as successful [50]. Notably, various types of cyclic CPPs and intracellular organelle targeting CPPs have been newly developed [51][52][53].…”

Section: The History Of Cpp Developmentmentioning

confidence: 99%

Cell-Penetrating Peptide-Based Delivery of Macromolecular Drugs: Development, Strategies, and Progress

et al. 2023

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Cell-penetrating peptides (CPPs), developed for more than 30 years, are still being extensively studied due to their excellent delivery performance. Compared with other delivery vehicles, CPPs hold promise for delivering different types of drugs. Here, we review the development process of CPPs and summarize the composition and classification of the CPP-based delivery systems, cellular uptake mechanisms, influencing factors, and biological barriers. We also summarize the optimization routes of CPP-based macromolecular drug delivery from stability and targeting perspectives. Strategies for enhanced endosomal escape, which prolong its half-life in blood, improve targeting efficiency and stimuli-responsive design and are comprehensively summarized for CPP-based macromolecule delivery. Finally, after concluding the clinical trials of CPP-based drug delivery systems, we extracted the necessary conditions for a successful CPP-based delivery system. This review provides the latest framework for the CPP-based delivery of macromolecular drugs and summarizes the optimized strategies to improve delivery efficiency.

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Identification of a novel cell-penetrating peptide derived from the capsid protein of chicken anemia virus and its application in gene delivery

Cited by 9 publications

References 51 publications

In silico identification and experimental validation of cellular uptake and intracellular labeling by a new cell penetrating peptide derived from CDN1

In silico identification and experimental validation of cellular uptake and intracellular labeling by a new cell penetrating peptide derived from CDN1

Emerging Landscape of Supercharged Proteins and Peptides for Drug Delivery

Cell-Penetrating Peptide-Based Delivery of Macromolecular Drugs: Development, Strategies, and Progress

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