Identification of a novel class of human adherent CD34− stem cells that give rise to SCID-repopulating cells

Kuçi, Selim; Wessels, Johannes T.; Bühring, Hans‐Jörg; Schilbach, Karin; Schumm, Michael; Seitz, Guido; Löffler, Jürgen; Bader, Peter; Schlegel, Paul G.; Niethammer, D.; Handgretinger, Rupert

doi:10.1182/blood-2002-03-0711

Cited by 95 publications

(74 citation statements)

References 29 publications

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“…Therefore, as the Lin -cell population in this study contained more LTC-IC but fewer CFC than CD133 + cells, these cells underwent slower proliferation upon TPOFLK synergism, while possibly expanding a more primitive HSPC pool than the CD133 + cell subset. This may be of particular importance, as a recent study by Kuci et al [54] demonstrated that a discrete CD34 -…”

Section: Cd34mentioning

confidence: 99%

Characterization of a Lineage‐Negative Stem‐Progenitor Cell Population Optimized for Ex Vivo Expansion and Enriched for LTC‐IC

2004

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Section: Cd34mentioning

confidence: 99%

Characterization of a Lineage‐Negative Stem‐Progenitor Cell Population Optimized for Ex Vivo Expansion and Enriched for LTC‐IC

2004

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“…CD133 þ cells in the peripheral blood have been shown to comprise early stem cells with substantial NOD/SCID mouse repopulating capacity and potential plasticity. 2 Large-scale immunomagnetic selection using a monoclonal anti-CD133 antibody allows to enrich sufficient numbers of hematopoietic progenitor cells to perform hematopoietic stem cell transplantation (HSCT). We performed a feasibility trial in 10 adult patients with leukemia to test whether sustained engraftment can be achieved with allogeneic CD133 þ purified stem cells.…”

mentioning

confidence: 99%

Rapid reconstitution of dendritic cells after allogeneic transplantation of CD133+ selected hematopoietic stem cells

Bornhäuser¹,

Eger²,

Oelschlaegel³

et al. 2004

Leukemia

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Hence, one may speculate that the genetic alterations detected in these cell lines may have been present in the primary tumors from which they arose, and could reflect events that are relevant to the pathogenesis of BL and cervical carcinoma, respectively.Today, continuous suspension cultures are a common research tool; so common, in fact, that we seldom reflect on their origin or genetic constitution. Using the molecular cytogenetic techniques SKY, array CGH, and FISH, we have provided a complete and definitive karyotype of the original BL-derived cell line, Raji. Our results reveal a number of novel genomic aberrations, concurrent with the hallmark t(8;14) translocation. The use of continuous human cell lines has played a major role in the advancement of our understanding of hematopoietic and other malignancies. An appreciation of the genetic anomalies of a cancer cell line may aid in the correct interpretation of data generated in such a model. The current results may thus serve as a valuable reference for future studies on BL.

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“…Interestingly, in vitro culture of an adherent fraction of CD133 + cells has been reported to give rise to a nonadherent CD133 + CD34 neg subset with SRC activity (Kuci et al, 2003). The existence of candidate human CD34 neg HSCs in human bone marrow is also supported by engraftment in the sheep xenogeneic transplant model (Zanjani et al, 1998).…”

Section: Phenotype and Function Of Tissue-specific Stem Cellsmentioning

confidence: 81%

Integrative molecular and developmental biology of adult stem cells

Bunting

Hawley

2003

Biology of the Cell

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Stem cells are believed to be important for regeneration of several adult tissues. Recently, adult stem cells with very broad differentiation potential have been identified although whether they represent vestigial primitive pluripotent stem cells or products of extremely rare de-differentiation events involving tissue-specific stem cells is not known. Transdifferentiation of tissue-specific stem cells across lineage boundaries has also been demonstrated but the relative inefficiency of the process in vivo, even in the presence of tissue damage, questions whether such a mechanism is of physiologic relevance. Interestingly, among adult stem cells, the capacity for lineage switching appears to be greatest in stem cells that can be cultured ex vivo for extended periods. If the normal cell fate decisions of diverse adult stem cell types could be reliably redirected at high frequency in situ, possible regenerative therapies for a wide variety of diseases could be envisioned. An integrated understanding of the transcriptional regulatory networks that comprise the various adult stem cell entities as well as the signaling pathways governing their differentiation into therapeutically useful cell types will facilitate clinical application of these exciting findings.

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Identification of a novel class of human adherent CD34− stem cells that give rise to SCID-repopulating cells

Cited by 95 publications

References 29 publications

Characterization of a Lineage‐Negative Stem‐Progenitor Cell Population Optimized for Ex Vivo Expansion and Enriched for LTC‐IC

Characterization of a Lineage‐Negative Stem‐Progenitor Cell Population Optimized for Ex Vivo Expansion and Enriched for LTC‐IC

Rapid reconstitution of dendritic cells after allogeneic transplantation of CD133+ selected hematopoietic stem cells

Integrative molecular and developmental biology of adult stem cells

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