Identification of a novel collagen-like peptide by high-throughput screening for effective wound-healing therapy

Du, Chunyu; Li, Ying; Xia, Xiaoling; Du, Erxia; Lin, Ye; Lian, Junyi; Ren, Chonghua; Li, Sheng; Wei, Wei; Qin, Yiru

doi:10.1016/j.ijbiomac.2021.01.104

Cited by 15 publications

(4 citation statements)

References 55 publications

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“…We picked out several differentially expressed genes with extensive interactions; for instance, Sparc , Vim , Col4a1 , Col6a1 , Col1a1 and Col3a1 . These genes are considered to relate to cell proliferation, bone mineralization and multipotent differentiation[ 41 - 43 , 51 - 54 ]. In Cd271 knockout MSCs, we verified by quantitative real-time PCR that these differentially expressed gene clusters had lower expression.…”

Section: Discussionmentioning

confidence: 99%

Single cell RNA sequencing reveals mesenchymal heterogeneity and critical functions of Cd271 in tooth development

Zhang¹,

Li²,

Zeng³

et al. 2023

World J Stem Cells

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BACKGROUND Accumulating evidence suggests that the maxillary process, to which cranial crest cells migrate, is essential to tooth development. Emerging studies indicate that Cd271 plays an essential role in odontogenesis. However, the underlying mechanisms have yet to be elucidated. AIM To establish the functionally heterogeneous population in the maxillary process, elucidate the effects of Cd271 deficiency on gene expression differences. METHODS p75NTR knockout ( Cd271 -/- ) mice (from American Jackson laboratory) were used to collect the maxillofacial process tissue of p75NTR knockout mice, and the wild-type maxillofacial process of the same pregnant mouse wild was used as control. After single cell suspension, the cDNA was prepared by loading the single cell suspension into the 10x Genomics Chromium system to be sequenced by NovaSeq6000 sequencing system. Finally, the sequencing data in Fastq format were obtained. The FastQC software is used to evaluate the quality of data and CellRanger analyzed the data. The gene expression matrix is read by R software, and Seurat is used to control and standardize the data, reduce the dimension and cluster. We search for marker genes for subgroup annotation by consulting literature and database; explore the effect of p75NTR knockout on mesenchymal stem cells (MSCs) gene expression and cell proportion by cell subgrouping, differential gene analysis, enrichment analysis and protein-protein interaction network analysis; understand the interaction between MSCs cells and the differentiation trajectory and gene change characteristics of p75NTR knockout MSCs by cell communication analysis and pseudo-time analysis. Last we verified the findings single cell sequencing in vitro . RESULTS We identified 21 cell clusters, and we re-clustered these into three subclusters. Importantly, we revealed the cell–cell communication networks between clusters. We clarified that Cd271 was significantly associated with the regulation of mineralization. CONCLUSION This study provides comprehensive mechanistic insights into the maxillary- process-derived MSCs and demonstrates that Cd271 is significantly associated with the odontogenesis in mesenchymal populations.

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Section: Discussionmentioning

confidence: 99%

Single cell RNA sequencing reveals mesenchymal heterogeneity and critical functions of Cd271 in tooth development

Zhang¹,

Li²,

Zeng³

et al. 2023

World J Stem Cells

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“…1 ) and only those earthworms were selected in which regeneration tissue has developed. Following this about 1 cm of regenerated tissue was harvested from earthworms to prepare earthworm granulation tissue homogenate as per method of [ 21 ]. Briefly the regenerated tissue harvested was homogenized in Tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) buffer (10 mM, pH 7.2) by mixing 1 part of regenerated tissue with four parts of buffer.…”

Section: Methodsmentioning

confidence: 99%

“…These studies have reported promising results in wound healing for diabetic patients. Among these traditional remedies, various products and extracts derived from earthworms have demonstrated multiple pharmacological actions beneficial for promoting wound healing, including antioxidant properties and modulation of inflammatory pathways [ 21 ]. Additionally, these studies have noted that earthworm protein components can enhance the expression of genes involved in wound healing [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Modulation of immune cum inflammatory pathway by earthworm granulation tissue extract in wound healing of diabetic rabbit model

M. E. Elkhalifa,

Ali,

Nabi

et al. 2024

Heliyon

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“…However, as with any emerging skincare trend, there are numerous aspects to consider before earthworm-based skincare products can be readily embraced by consumers (Du et al, 2021;Maser & Rice, 1963). Firstly, the scientific community faces the challenge of unraveling the mechanisms through which earthworm compounds exert their anti-aging effects on human skin.…”

Section: Introductionmentioning

confidence: 99%

Earthworms: Anti-Aging Skin Care Miracle

Christine,

Damayanti

2023

jppipa, pendidikan ipa, fisika, biologi, kimia

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This studi aims to exploration of diverse natural sources for potential remedies. Earthworms, often overlooked in the realm of skincare, have gained attention for their potential role in anti-aging treatments. The methodological approach used in this research is systematic review. This research focuses on randomized controlled trials published on the website from 2017 to 2022. Researchers will screen, extract the data, and cross-check the results. This research from Science Direct, ProQuest, CINAH, Pubmed and Clinical Trials.gov. This research will be conducted by collecting and evaluating various relevant literature sources to identify previous research that has been conducted on this topic. The multifaceted benefits they offer, coupled with their regenerative properties, make them a subject worthy of continued scientific exploration.

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Identification of a novel collagen-like peptide by high-throughput screening for effective wound-healing therapy

Cited by 15 publications

References 55 publications

Single cell RNA sequencing reveals mesenchymal heterogeneity and critical functions of Cd271 in tooth development

Single cell RNA sequencing reveals mesenchymal heterogeneity and critical functions of Cd271 in tooth development

Modulation of immune cum inflammatory pathway by earthworm granulation tissue extract in wound healing of diabetic rabbit model

Earthworms: Anti-Aging Skin Care Miracle

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