2007
DOI: 10.1074/jbc.m607396200
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Identification of a Novel Domain at the N Terminus of Caveolin-1 That Controls Rear Polarization of the Protein and Caveolae Formation

Abstract: When cells are migrating, caveolin-1, the principal protein component of caveolae, is excluded from the leading edge and polarized at the cell rear. The dynamic feature depends on a specific sequence motif that directs intracellular trafficking of the protein. Deletion mutation analysis revealed a putative polarization domain at the N terminus of caveolin-1, between amino acids 32-60. Alanine substitution identified a minimal sequence of 10 residues ( 46 TKEIDLVNRD 55 ) necessary for caveolin-1 rear polarizati… Show more

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Cited by 37 publications
(51 citation statements)
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“…6H, down-regulation of caveolin-1 causes up to 40% reduction in migration compared with that of control siRNA-treated cells. This observation is consistent with our previous results demonstrating that both knockdown of caveolin-1 using caveolin-1 siRNA and genetic depletion of the caveolin-1 protein results in impeded cell migration (18,21).…”
Section: Spreading Area (Fold)supporting
confidence: 93%
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“…6H, down-regulation of caveolin-1 causes up to 40% reduction in migration compared with that of control siRNA-treated cells. This observation is consistent with our previous results demonstrating that both knockdown of caveolin-1 using caveolin-1 siRNA and genetic depletion of the caveolin-1 protein results in impeded cell migration (18,21).…”
Section: Spreading Area (Fold)supporting
confidence: 93%
“…In the past, we reported that caveolin-1 is also an important regulator of cell polarity and directional movement (18,21), and recently, it was shown that caveolin-1 exerts this effect on cell movement by impacting the activity of Rho GTPases (74). Our current data show that increases in caveolin-1 expression follow classically described cellular changes associated with EMT (including changes in cell morphology and expression of the cadherins and fibronectin).…”
Section: Discussionsupporting
confidence: 73%
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“…However, it is still controversial about the expression and distribution of CAV1 in cell migration. CAV1 preferentially accumulates at the cell front in transmigrating endothelial cells and neurons, but at the rear of migrating fibroblasts neurons and endothelial cell (Parat et al, 2003;Sun et al, 2007;Lentini et al, 2008). Unlike for the cells we have mentioned, polarization and relocation to the trailing edge of CAV1 were not observed in migrating metastatic cells (Urra et al, 2012).…”
Section: Discussionmentioning
confidence: 55%
“…It is still controversial about the role of CAV1 in tumor progression, especially for cell migration. In non-metastatic cells, for instance, fibroblasts, neurons and endothelial cells, CAV1 accumulates at the rear of the migrating cells (Beardsley et al, 2005;Sun et al, 2007;Lentini et al, 2008), while in metastatic cells, such as MDA-MB-231 human breast cancer cells and B16-F10 mouse melanoma, CAV1 does not accumulate at the edge during migration (Urra et al, 2012).…”
Section: New Insights Into 4-amino-2-tri-fluoromethyl-phenyl Ester Inmentioning
confidence: 99%