Identification of a novel endochitinase from a marine bacterium Vibrio proteolyticus strain No. 442

Itoi, Shiro; Kanomata, Yuna; Koyama, Yuki; Kadokura, Kazunari; Uchida, Shinsuke; Nishio, Takeshi; Oku, Tatsuo; Sugita, Haruo

doi:10.1016/j.bbapap.2007.06.003

Cited by 27 publications

(26 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…None of these strains could produce alginase, phosphatase or xylanase. Exoenzyme production in vibrios is well reported (Itoi et al 2007;Maureen et al 1977;Samuel and Gabriel 1981;Young andBroadbent 1982, Raghul andSarita 2011); these enzymes play a central role in the recycling of organic carbon and nitrogen compounds in the marine environment. Hydrolytic enzymes such as amylases, caseinases, lipases, DNases, pectinases, cellulases and gelatinases exhibit the versatility of these heterotropic vibrios in utilizing several types of carbon sources as substrates.…”

Section: Isolation and Characterization Of Pva-degrading Microorganismsmentioning

confidence: 87%

Biodegradation of polyvinyl alcohol-low linear density polyethylene-blended plastic film by consortium of marine benthic vibrios

Raghul

Bhat

Chandrasekaran

et al. 2013

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

Marine bacteria, Vibrio alginolyticus and Vibrio parahemolyticus isolated from sediments were evaluated for their ability as a consortia, to degrade polyvinyl alcohol-low linear density polyethylene (PVA-LLDPE)-blended plastic films in shake flask conditions at 120 rpm at 37°C over 15 weeks. Results indicated that relatively 20 % decrease in tensile strength of the film could be achieved with 25 and 30 % blend of PVA in the PVA-LLDPE plastic film compared to other ratios. Micrographs obtained with scanning electron microscope showed visible cracks and grooves on the surface of the PVA-LLDPE blend film after 15 weeks of incubation with bacterial consortium. The decrease in tensile strength of the PVAblended plastic films after treatment and the results of the scanning electron microscopic analysis evidence that the consortium could cause degradation of PVA-LLDPE plastic blends compared to suitable controls. This is the first report on polyvinyl alcohol degrading Vibrio sp. from marine sediments and its application in microbial degradation of polyvinyl alcohol-low linear density polyethylene plastic blends. The study indicated potential of marine benthic vibrios that have novel enzymes and unique characteristics for application in bioremediation and solid waste management particularly in handling synthetic polymers such as PVA-blended plastic films.

show abstract

Section: Isolation and Characterization Of Pva-degrading Microorganismsmentioning

confidence: 87%

Biodegradation of polyvinyl alcohol-low linear density polyethylene-blended plastic film by consortium of marine benthic vibrios

Raghul

Bhat

Chandrasekaran

et al. 2013

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

show abstract

“…Chitinolytic bacteria, which are very abundant and widely distributed in marine environments, are responsible for rapid degradation of chitin in aqueous systems (Keyhani and Roseman 1996). Accordingly, there are numerous reports about the isolation of bacterial chitinase from marine environments (Tsujibo et al 1998;Itoi et al 2007;Stefanidi and Vorgias 2008). Because most industrial processes are carried out in harsh physicochemical conditions, which may not be definitively adjusted to the optimal conditions required for the activity of the available enzymes, it would be of great value to have enzymes that demonstrate optimal activities in wide ranges of salinity, temperature and pH values.…”

Section: Introductionmentioning

confidence: 99%

A novel halo-alkali-tolerant and thermo-tolerant chitinase from Pseudoalteromonas sp. DC14 isolated from the Caspian Sea

Makhdoumi

Dehghani-Joybari

Mashreghi

et al. 2015

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

A novel halo-alkali and thermo-tolerant chitinase was obtained from an isolated strain found in the Caspian Sea. The effects of media composition and various fermentation conditions for the optimization of chitinase production were studied one factor at a time and by response surface methodology. The novel strain, which is designated as strain DC14 and phylogenetically related to the genus Pseudoalteromonas, produced chitinase after 72 h under the following optimal conditions: glucose 1 % (w/v), ammonium sulphate 0.2 % (w/v), chitin 1.07 % (w/ v), pH 8, NaCl 10 % (w/v), inoculums size 2.5 % (v/v), temperature 30°C, CaCl 2 3 mM and MgCl 2 4 mM. Using the statistical optimization method, chitinase production was found to increase from 2.30 to 21.90 U/dl. The enzyme showed maximum activity at 40°C, pH 9 and 10 % NaCl. It was stable in a wide range of temperature from 15 to 65°C, pH from pH 7 to 11 and NaCl concentration from 0 to 15 % (w/v). The molecular weight of the enzyme was estimated by SDS-PAGE to be about 65 kDa. With regard to the halo-alkali and thermo-stable properties of this enzyme, it has potential industrial activity.

show abstract

“…Different bacteria seem to secrete different forms of chitinases [2, 11–17]. For example, Serratia marcescens produces three chitinases: ChiA, ChiB, and ChiC for a synergistic degradation of chitin [18, 19], whereas Vibrio harveyi (formerly Vibrio carchariae ) mainly expresses chitinase A [2].…”

Section: Introductionmentioning

confidence: 99%

Substrate binding modes and anomer selectivity of chitinase A from Vibrio harveyi

2009

View full text Add to dashboard Cite

High-performance liquid chromatography mass spectrometry (HPLC MS) was employed to assess the binding behaviors of various substrates to Vibrio harveyi chitinase A. Quantitative analysis revealed that hexaNAG preferred subsites −2 to +2 over subsites −3 to +2 and pentaNAG only required subsites −2 to +2, while subsites −4 to +2 were not used at all by both substrates. The results suggested that binding of the chitooligosaccharides to the enzyme essentially occurred in compulsory fashion. The symmetrical binding mode (−2 to +2) was favored presumably to allow the natural form of sugars to be utilized effectively. Crystalline α chitin was initially hydrolyzed into a diverse ensemble of chitin oligomers, providing a clear sign of random attacks that took place within chitin chains. However, the progressive degradation was shown to occur in greater extent at later time to complete hydrolysis. The effect of the reducing-end residues were also investigated by means of HPLC MS. Substitutions of Trp275 to Gly and Trp397 to Phe significantly shifted the anomer selectivity of the enzyme toward β substrates. The Trp275 mutation modulated the kinetic property of the enzyme by decreasing the catalytic constant (kcat) and the substrate specificity (kcat/Km) toward all substrates by five- to tenfold. In contrast, the Trp397 mutation weakened the binding strength at subsite (+2), thereby speeding up the rate of the enzymatic cleavage toward soluble substrates but slowing down the rate of the progressive degradation toward insoluble chitin.

show abstract

Identification of a novel endochitinase from a marine bacterium Vibrio proteolyticus strain No. 442

Cited by 27 publications

References 36 publications

Biodegradation of polyvinyl alcohol-low linear density polyethylene-blended plastic film by consortium of marine benthic vibrios

Biodegradation of polyvinyl alcohol-low linear density polyethylene-blended plastic film by consortium of marine benthic vibrios

A novel halo-alkali-tolerant and thermo-tolerant chitinase from Pseudoalteromonas sp. DC14 isolated from the Caspian Sea

Substrate binding modes and anomer selectivity of chitinase A from Vibrio harveyi

Contact Info

Product

Resources

About